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Fance (NM_001163819) Mouse Tagged ORF Clone
CAT＃: MR213007
- TrueORF®
Fance (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group E (Fance), transcript variant 1
ORF Plasmid: tGFP
"NM_001163819" in other vectors (4)
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CNY 3,920.00
CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 2810451D06Rik; AI415634; AW209126 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR213007 representing NM_001163819
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC CTGGCGACGCCGGGGTCGGACTCCGTAGCCTCGGCCGGAGCGGCGCCCTGGGCGAGCCTGGAGGCCCCTG CCCGCCTCCTGCTTCAGGCGCTACAGGCAGGGCCTGAGGGTGCGCGGCGCGGGCTAGGGGTTCTGCGCGC CCTCGGCCGCCGCGCGGAGCACTTCCCTTGGGATGGCTTCCTGGAGGCGCTGGGCCACTTAGAGCCCGAG GTGCGGGGCCCCGACGGCCGCCTAGAGCTGGTCCCACTGTTGCTCCGGCTGCCTGGGGTCTGCCAGAAGA ACCTGCTGTCCCTGCTGCTGGCTCTCCTACCATCCTTACCTGAAAGCGGACTCCGCTCTGTGCTGCAGCT CCACCATCAGGATGTGTCCTCTACCACTGATGCCTGGCTCCATGCCCTGGGGGAGCTGCTGCGAAGGGAT GTGGGGGTTGGAGTCGCTGAGGGATCTTCTCCATTGACCAGAAGCTGTCAGTTACAGCTCCGGGACCTGT GTGGGCGGCTGGGCCAAGGGGGGAGGGGGCTGAAACTGGCCCTGGCTCCAGATCCTGAACAAGAGGACAG ACTCTCACAGCTTTGCGGGAAACGGACGAAAGAGCCAGAAGAGGCTGCCAGCCCTGAGTCAGAGAGATCC CCTAAAAGGTTCCGGGGCTGTGAGGAGGCGGTGGAAGGAAAGGAACCGGAGGAGAGACCCACGCTGGAGT CGCTGGGATCCCCACCAGATGCAGGAGGCGTGTTGCCTGACACTGACGCCCAGGCTCCGGAGACTGGCCC TGGCGTGGAGGGTCCCAAGGGTCCCGCTGAGAGTGTGGAGTTGCCCAAAGTTGTCCAGGACCAGGTGCCC AGGCTGCAGCTGCTGCTGAAGGCCTTCCAGGAGGGGCTGGAGGGTCAGGAGAAGCCCCTAGTGGACCTGC AGTTTCTTCATGAATGTAGTCCCAGCGAGATGGAGTTGCTATGCAGCGAACTACAGCTACCCCAGCTCCC TGACGGAGGTCTCCTGCAGCTCTGCAGCCACCTGATGGGTCTCACACCAGCCCTCAGCCTCAGCAATGCC TCTGTGCTGGCCAGGAGCCTCTTTCTTGACCGGATCCGCTCCCTGCCGTCTTCTGCCTCCAGGCTTCTCA GAGTGGCCCTCGTCTCCTTCTGTGTAAAGTACACCTACGCCATCTGCAGGGCTGTCCTCTGTCCCTTGCT CCAGGACCCTCGTGTAGGTCCTGCGCAGACCGAGTTACTGTGTTCCCTCATAAAGGATGAGTCCCTGGAG TCAGACATGCAGGTCCAGATTTTGGGGCAGGTCCTGGAGCTGGCCTGGCGAGAAGAGACGTTCCTGGTGT TGCAGACGCTCCTGGAACGGCAGGTAGAGATGACCCCTGAGGTGTTCAGTGTCTTAGTGCAGAGGCTCTG CAAAGAGGGACCAGCAGCCACTACCTCCATGGCCTATGCCAAGCTGATGCTGACGGTGATGACCAAGTAC CAGACCAGTATCACAGAGCAGCAGAGCCTGGACCTGGCTGTGGCCCTAGAGCCCAACGCCACCTTCCTGA AGAAGGCCCTGCAAGCAGCGCTGAGACATGTGACCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR213007 representing NM_001163819
Red=Cloning site Green=Tags(s) LATPGSDSVASAGAAPWASLEAPARLLLQALQAGPEGARRGLGVLRALGRRAEHFPWDGFLEALGHLEPE VRGPDGRLELVPLLLRLPGVCQKNLLSLLLALLPSLPESGLRSVLQLHHQDVSSTTDAWLHALGELLRRD VGVGVAEGSSPLTRSCQLQLRDLCGRLGQGGRGLKLALAPDPEQEDRLSQLCGKRTKEPEEAASPESERS PKRFRGCEEAVEGKEPEERPTLESLGSPPDAGGVLPDTDAQAPETGPGVEGPKGPAESVELPKVVQDQVP RLQLLLKAFQEGLEGQEKPLVDLQFLHECSPSEMELLCSELQLPQLPDGGLLQLCSHLMGLTPALSLSNA SVLARSLFLDRIRSLPSSASRLLRVALVSFCVKYTYAICRAVLCPLLQDPRVGPAQTELLCSLIKDESLE SDMQVQILGQVLELAWREETFLVLQTLLERQVEMTPEVFSVLVQRLCKEGPAATTSMAYAKLMLTVMTKY QTSITEQQSLDLAVALEPNATFLKKALQAALRHVTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001163819 |
| ORF Size | 1578 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001163819.1, NP_001157291.1 |
| RefSeq Size | 2016 bp |
| RefSeq ORF | 1581 bp |
| Locus ID | 72775 |
| MW | 56.9 kDa |
| Gene Summary | This gene encodes the complementation group E subunit of the multimeric Fanconi anemia (FA) nuclear complex composed of proteins encoded by over ten Fanconi anemia complementation (FANC) group genes: FANCA, FANCB, FANCC, FANCD1 (also called BRCA2), FANCD2, FANCE, FANCF, FANCG, FANCI, FANCJ (also called BRIP1), FANCL, FANCM and FANCN (also called PALB2). The FA complex is necessary for protection against DNA damage. This gene product is required for the nuclear accumulation of FANCC and provides a critical bridge between the FA complex and FANCD2. Defects in the related human gene are a cause of Fanconi anemia, a genetically heterogeneous recessive disorder characterized by cytogenetic instability, hypersensitivity to DNA crosslinking agents, increased chromosomal breakage, and defective DNA repair. Translation of this protein is initiated at a non-AUG (CUG) start codon, which is inferred from the related human gene and the notion that this protein is functionally indispensable. Multiple transcript variants encoding different isoforms have been identified. [provided by RefSeq, Aug 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC217474 | Fance (untagged) - Mouse Fanconi anemia, complementation group E (Fance), transcript variant 1, (10ug) |
CNY 6,750.00 |
|
| MG213007 | Fance (tGFP-tagged) - Mouse Fanconi anemia complementation group E (Fance) transcript variant 1, (10ug) |
CNY 5,040.00 |
|
| MR213007L3 | Lenti ORF clone of Fance (Myc-DDK-tagged) - Mouse Fanconi anemia, complementation group E (Fance), transcript variant 1 |
CNY 6,460.00 |
|
| MR213007L4 | Lenti ORF clone of Fance (mGFP-tagged) - Mouse Fanconi anemia, complementation group E (Fance), transcript variant 1 |
CNY 6,460.00 |
