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Dis3l2 (NM_001172157) Mouse Tagged ORF Clone
CAT＃: MR216397
- TrueORF®
Dis3l2 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 1
ORF Plasmid: tGFP
"NM_001172157" in other vectors (3)
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CNY 8,840.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 4930429A22Rik; 8030493P09Rik |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR216397 representing NM_001172157
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAACCATCCTGACTACAAGCTGAACCTTCGGTCTCCGGGGACCCCCAGAGGTGTGTCCTCTGTGGTTG GCCCGAGTGCTGTTGGTGCTTCGCCAGGTGACAAAAAGTCAAAGAACAAGTCCATGCGAGGGAAGAAAAA GAGCATATTTGAAACCTACATGTCCAAGGAGGATGTTTCAGAAGGCTTGAAGAGAGGAACACTTATCCAG GGTGTATTGAGAATCAACCCAAAGAAGTTTCATGAAGCCTTCATTCCTTCTCCGGATGGTGATCGGGACA TTTTTATTGATGGAGTTGTTGCTCGTAATAGAGCCTTAAATGGGGACCTTGTGGTTGTAAAACTGCTTCC TGAGGATCAGTGGAAGCCAAGGATAACCTTGTCTCTGCCTGGAGTGCTGGGATTACAGGCAGTTAAACCA GAGAGCAATGACAAAGAAATAGAAGCTACTTATGAAGCTGACATCCCTGAAGAGGGCTGTGGACATCACC CCCTGCAGCAGTCCCGGAAAGGCTGGAGTGGTCCTGATGTCATTATAGAGGCTCAGTTTGATGACAGCGA CTCAGAAGATAGACATGGCAACACCAGTGGCCTGGTTGATGGTGTTAAGAAATTGTCAATCTCTACTCCT GACAGAGGAAAAGAAGATTCTAGTACTCCAGTTATGAAAGATGAGAACACCCCCATACCACAGGACACAA GAGGCTTATCAGAGAAGTCACTTCAGAAATCAGCAAAGGTGGTTTACATCTTGGAGAAAAAGCATTCTCG AGCAGCAACTGGCATCCTGAAACTCTTGGCTGATAAGAACAGTGACCTGTTTAAGAAATACGCCCTGTTT TCTCCTTCAGACCACCGAGTACCTAGAATTTACGTACCTCTCAAGGACTGTCCCCAGGACTTCATGACCC GACCTAAAGACTTTGCCAACACGCTGTTCATCTGCCGCATCATAGACTGGAAGGAGGACTGTAATTTTGC CCTGGGGCAACTGGCTAAGAGTCTTGGGCAGGCTGGTGAAATCGAGCCTGAAACAGAAGGGATACTGACA GAATATGGTGTGGACTTCTCTGATTTCTCTTCAGAAGTTCTTGAATGTCTCCCTCAAAGCCTGCCCTGGA CAATCCCACCTGATGAGGTGGGCAAGAGAAGAGACCTAAGGAAAGACTGTATCTTCACCATTGATCCATC AACTGCTCGCGACCTTGATGATGCCCTCGCCTGCAGGCGGCTCACTGATGGCACCTTCGAAGTGGGCGTC CACATCGCCGATGTGAGTTACTTTGTTCCTGAGGGATCCTCTTTGGATAAAGTAGCTGCTGAGAGAGCCA CAAGTGTCTACTTGGTCCAGAAGGTGGTCCCCATGCTTCCCAGGCTTCTGTGTGAGGAACTCTGCAGCCT CAACCCCATGACTGACAAGCTGACCTTCTCTGTGATCTGGAAGCTGACCCCTGAAGGCAAGATCCTTGAA GAGTGGTTTGGCCGCACTATCATCCGTTCTTGCACCAAACTGAGCTACGACCATGCCCAGAGCATGATCG AAAATCCAACTGAGAAGATCCCTGAGGAAGAGCTTCCCCCAATTTCTCCAGAGCACAGCGTCGAGGAGGT GCACCAGGCAGTCCTGAACCTGCACAGCATTGCAAAGCAACTCCGCCGCCAGCGCTTTGTAGATGGCGCA CTCCGTTTAGATCAGCTGAAGCTTGCTTTTACTCTGGACCATGAGACTGGACTGCCTCAAGGATGTCACA TCTATGAGTACCGAGACAGCAACAAGCTTGTAGAGGAGTTCATGCTCCTGGCCAACATGGCGGTGGCCCA CAAGATCTTCCGCACCTTCCCTGAGCAGGCCCTGCTGCGCCGGCATCCCCCACCACAGACGAAGATGCTC AGTGACCTGGTGGAGTTCTGTGACCAGATGGGGCTGCCCATGGATGTCAGCTCTGCAGGGGCCCTAAATA AAAGCCTGACTAAGACATTTGGAGATGACAAGTACTCTCTGGCCCGGAAGGAGGTGCTCACCAACATGTA CTCCCGGCCCATGCAGATGGCACTGTACTTCTGCTCTGGGATGCTGCAGGACCAGGAGCAGTTCCGGCAT TATGCTCTCAACGTTCCCCTCTACACACACTTCACCTCTCCCATCCGCCGCTTTGCTGACGTCATAGTGC ACCGCCTCCTGGCTGCTGCTCTGGGCTACAGTGAACAGCCAGATGTGGAGCCTGATACCCTACAGAAGCA AGCTGACCACTGCAATGACCGTCGCATGGCTTCCAAACGTGTGCAGGAGCTCAGCATCGGCCTCTTCTTC GCAGTTCTAGTAAAGGAGAGTGGCCCCCTGGAGTCCGAAGCCATGGTGATGGGTGTCCTGAACCAAGCTT TCGACGTGCTGGTGCTGCGCTTTGGGGTGCAGAAGCGCATCTACTGCAATGCACTGGCCCTGCGATCCTA CAGCTTCCAGAAGGTGGGGAAGAAGCCAGAGCTCACTCTTGTTTGGGAGCCTGATGACCTTGAAGAGGAG CCAACACAGCAGGTCATCACCATCTTCAGCCTGGTGGATGTGGTCCTGCAGGCAGAGGCCACAGCCCTCA AGTACAGTGCTATCCTGAAGCGACCAGGCCTGGAGAAGGCGTCTGATGAGGAGCCTGAGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR216397 representing NM_001172157
Red=Cloning site Green=Tags(s) MNHPDYKLNLRSPGTPRGVSSVVGPSAVGASPGDKKSKNKSMRGKKKSIFETYMSKEDVSEGLKRGTLIQ GVLRINPKKFHEAFIPSPDGDRDIFIDGVVARNRALNGDLVVVKLLPEDQWKPRITLSLPGVLGLQAVKP ESNDKEIEATYEADIPEEGCGHHPLQQSRKGWSGPDVIIEAQFDDSDSEDRHGNTSGLVDGVKKLSISTP DRGKEDSSTPVMKDENTPIPQDTRGLSEKSLQKSAKVVYILEKKHSRAATGILKLLADKNSDLFKKYALF SPSDHRVPRIYVPLKDCPQDFMTRPKDFANTLFICRIIDWKEDCNFALGQLAKSLGQAGEIEPETEGILT EYGVDFSDFSSEVLECLPQSLPWTIPPDEVGKRRDLRKDCIFTIDPSTARDLDDALACRRLTDGTFEVGV HIADVSYFVPEGSSLDKVAAERATSVYLVQKVVPMLPRLLCEELCSLNPMTDKLTFSVIWKLTPEGKILE EWFGRTIIRSCTKLSYDHAQSMIENPTEKIPEEELPPISPEHSVEEVHQAVLNLHSIAKQLRRQRFVDGA LRLDQLKLAFTLDHETGLPQGCHIYEYRDSNKLVEEFMLLANMAVAHKIFRTFPEQALLRRHPPPQTKML SDLVEFCDQMGLPMDVSSAGALNKSLTKTFGDDKYSLARKEVLTNMYSRPMQMALYFCSGMLQDQEQFRH YALNVPLYTHFTSPIRRFADVIVHRLLAAALGYSEQPDVEPDTLQKQADHCNDRRMASKRVQELSIGLFF AVLVKESGPLESEAMVMGVLNQAFDVLVLRFGVQKRIYCNALALRSYSFQKVGKKPELTLVWEPDDLEEE PTQQVITIFSLVDVVLQAEATALKYSAILKRPGLEKASDEEPED TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172157 |
| ORF Size | 2652 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172157.1, NP_001165628.1 |
| RefSeq Size | 3171 bp |
| RefSeq ORF | 2655 bp |
| Locus ID | 208718 |
| UniProt ID | Q8CI75 |
| MW | 99.7 kDa |
| Gene Summary | 3'-5'-exoribonuclease that specifically recognizes RNAs polyuridylated at their 3' end and mediates their degradation. Component of an exosome-independent RNA degradation pathway that mediates degradation of both mRNAs and miRNAs that have been polyuridylated by a terminal uridylyltransferase, such as ZCCHC11/TUT4. Mediates degradation of cytoplasmic mRNAs that have been deadenylated and subsequently uridylated at their 3'. Mediates degradation of uridylated pre-let-7 miRNAs, contributing to the maintenance of embryonic stem (ES) cells. Essential for correct mitosis, and negatively regulates cell proliferation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG216397 | Dis3l2 (tGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2) transcript variant 1, (10ug) |
CNY 9,690.00 |
|
| MR216397L3 | Lenti ORF clone of Dis3l2 (Myc-DDK-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 1 |
CNY 10,740.00 |
|
| MR216397L4 | Lenti ORF clone of Dis3l2 (mGFP-tagged) - Mouse DIS3 mitotic control homolog (S. cerevisiae)-like 2 (Dis3l2), transcript variant 1 |
CNY 10,740.00 |
