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Clasp2 (NM_029633) Mouse Tagged ORF Clone
CAT＃: MR217994
- TrueORF®
Clasp2 (Myc-DDK-tagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1
ORF Plasmid: tGFP
"NM_029633" in other vectors (4)
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CNY 12,830.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1500004F14Rik; 8030404L10Rik; C77448; CLASP2beta; mKIAA0627 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR217994 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGCGGCTGATTTGCAAACGGATCTGTGATTATAAAAGCTTTGATGATGAGGAATCAGTGGATGGAA ATAGGCCGTCGTCAGCTGCTTCAGCCTTCAAGGTTCCTGCACCTAAAACACCTGGGAATCCTGTCAGCAG TGCAAGAAAGCCTGGCTCAGCAGGTGGCCCTAAGGTTGGAGGTCCTTCTAAAGAAGGAGGGGCTGGAGCA GTTGATGAAGATGACTTTATAAAAGCTTTTACAGATGTTCCTTCTGTTCAGATCTATTCTAGTCGAGAAC TTGAAGAGACGTTAAATAAGATCAGGGAAATTTTGTCAGATGACAAACATGACTGGGACCAGCGTGCCAA TGCGCTTAAGAAAATCAGATCACTGCTTGTTGCTGGAGCCGCACAGTATGATTGCTTTTTCCAGCACCTA CGTTTGTTGGATGGAGCGCTTAAGCTGTCAGCTAAGGACCTCAGATCCCAGGTGGTCAGGGAAGCTTGCA TCACTGTTGCTCACCTTTCAACAGTCTTGGGAAACAAATTTGATCACGGCGCTGAAGCCATCGTCCCTAC GCTTTTTAATCTTGTCCCCAATAGTGCCAAAGTCATGGCGACCTCTGGATGTGCAGCAATCAGATTTATT ATTCGGCATACCCATGTACCTAGACTTATTCCTTTAATAACAAGCAACTGCACATCAAAGTCAGTTCCTG TAAGGAGACGTTCATTTGAATTTTTAGATTTGCTGCTGCAAGAATGGCAGACTCATTCACTGGAAAGACA TGCAGCTGTTTTGGTTGAAACGATTAAGAAGGGCATTCATGATGCTGATGCTGAGGCCAGAGTGGAGGCA AGGAAGACATACATGGGCCTTAGGAACCACTTTCCTGGTGAAGCTGAAACATTGTACAACTCCCTTGAGC CATCATATCAGAAAAGTCTTCAAACTTACTTAAAGAGTTCTGGAAGTGTAGCTTCTCTTCCGCAGTCAGA CAGGTCCTCATCCAGCTCACAAGAAAGTCTCAATCGTCCTTTTTCTTCTAAATGGTCAACAGCAAATCCT TCAACTGTAGCTGGAAGAGTATCTGTGGGAGGCAGCAAAGCCAACCCCCTTCCAGGAAGCCTGCAGCGTT CTCGAAGTGACATTGATGTGAATGCGGCAGCTGGTGCCAAGGCTCATCATGCTGCTGGGCAGGCGGTGCG AAGTGGGCGCTTAGGTGCAGGTGCCCTGAACCCAGGCTCCTATGCATCACTAGAGGATACTTCTGACAAG ATGGATGGAACAGCATCTGATGATGGTCGGGTGAGAGCCAAGCTTTCTACACCGCTTGTTGCTGTGGGAA ATGCCAAGACCGACTCTAGAGGGAGAAGCCGGACAAAAATGGTGTCTCAGTCCCAGCCTGGCAGCCGATC TGGGTCTCCAGGAAGAGTTCTAACCACAACAGCCCTCTCTACTGTGAGCTCTGGTGCTCAACGAGTCCTG GTCAATTCAGCTTCAGCACAGAAGAGAAGCAAGATCCCAAGGAGCCAGGGCTGCAGCAGAGAGGCCAGCC CATCTCGGCTCTCAGTGGCCCGGAGCAGCCGTATTCCTCGGCCGAGTGTGAGTCAAGGCTGTAGCCGGGA AGCCAGCAGAGAGAGCAGCAGGGACACGAGTCCGGTGCGCTCCTTCCAGCCGCTGGGTCCGGGATATGGG ATCAGCCAGTCCAGCCGGTTGTCGTCCTCTGTCAGTGCCATGCGAGTCCTAAACACAGGCTCCGATGTGG AGGAGGCAGTAGCTGATGCCCTGCTCTTAGGAGACATACGGACTAAGAAAAAACCTGCTCGAAGAAGGTA TGAATCATATGGAATGCACTCAGATGATGATGCCAACAGCGATGCCTCTAGTGCGTGTTCAGAACGCTCC TATAGCTCTCGAAATGGTAGTATTCCTACCTACATGAGACAGACAGAAGACGTGGCAGAAGTCCTCAACA GATGTGCTAGCTCCAATTGGTCAGAGAGGAAAGAAGGCCTCTTGGGTCTGCAGAACTTGTTAAAAAACCA GAGAACGCTAAGTCGAGTTGAACTGAAAAGATTATGTGAAATTTTCACAAGAATGTTTGCAGATCCTCAT GGCAAGGTGTTCAGCATGTTCTTGGAGACTCTAGTAGATTTCATACAAGTCCACAAAGATGATCTTCAAG ATTGGTTGTTTGTTCTGCTGACACAGCTGCTGAAAAAAATGGGTGCTGATTTGCTTGGCTCTGTTCAGGC AAAAGTTCAGAAAGCCCTTGATATTACAAGAGAGTCTTTTCCAAATGATCTTCAGTTTAATATCCTAATG AGATTTACAGTTGACCAGACCCAAACGCCAAGCTTGAAGGTAAAGGTGGCTATCCTTAAGTACATAGAAA CTCTGGCAAAGCAGATGGACCCAAGAGATTTTACAAATTCCAGTGAAACTCGCCTGGCAGTGTCTCGGGT CATCACTTGGACGACAGAGCCCAAAAGCTCTGATGTTCGGAAGGCAGCGCAGTCAGTGCTGATTTCTTTA TTTGAACTCAATACCCCAGAGTTTACAATGTTACTAGGAGCTTTACCAAAAACTTTCCAGGATGGTGCTA CTAAACTTCTTCACAATCACCTCCGGAACACTGGCAATGGCACCCAGAGTTCCATGGGGAGTCCTTTGAC GAGACCAACACCTCGGTCACCAGCCAACTGGTCCAGTCCTCTTACTTCTCCTACCAACACGTCTCAGAAT ACGTTATCTCCAAGTGCATTTGATTACGATACAGAGAACATGAATTCTGAAGACATTTATAGCTCCCTTA GAGGCGTCACTGAGGCAATCCAGAATTTCAGCTTCAGAAGCCAAGAAGATATGAGTGAGCCAGTGAGGAG GGACCCTAAAAAGGAGGATGGTGACACAATATGTAGTGGTCCTGGGATGTCAGATCCAAGAGCAGGAGGT GATGCTGCTGACGGCAGCCAGCCAGCTCTGGATAATAAAGCATCGTTGCTCCACTCAATGCCACTCCACT CCTCTCCACGCTCCCGTGACTATAACCCATATAACTACTCAGATAGCATCAGTCCTTTCAACAAGTCTGC CCTCAAGGAAGCCATGTTTGATGATGACGCCGACCAATTTCCTGATGATCTTTCCTTAGACCATTCTGAC CTAGTTGCAGAGTTGTTGAAGGAGCTGTCTAATCATAATGAACGTATAGAAGAAAGAAAAATTGCCCTGT ATGAACTCATGAAGCTAACCCAGGAAGAATCTTTCAGTGTTTGGGATGAACACTTCAAAACAATATTATT GTTATTGCTTGAGACCCTTGGGGATAAAGAGCCTACAATCCGGGCCTTGGCATTAAAAGTTTTAAAAGAA ATCTTAAGGCATCAACCAGCAAGATTCAAAAACTATGCAGAACTAACTGTCATGAAAACACTGGAAGCAC ATAAAGATCCTCACAAAGAGGTGGTGAGATCTGCTGAGGAAGCTGCCTCCGTATTGGCTACTTCCATTAG TCCAGAGCAGTGCATCAAAGTGCTTTGTCCCATCATACAAACCGCTGACTACCCTATTAATCTGGCTGCA ATCAAAATGCAAACAAAAGTGATAGAGAGAGTATCCAAGGAGACCCTTAACATGCTCTTACCAGAGATCA TGCCGGGTCTAATACAGGGTTATGATAATTCAGAAAGCAGTGTCCGGAAAGCTTGTGTCTTCTGCCTGGT GGCTGTCCATGCAGTGATTGGTGATGAACTAAAGCCACATCTCAGTCAACTCACTGGTAGTAAAATGAAG CTGCTGAACCTTTACATCAAGCGTGCACAGACGGGCTCTGCAGGCGCGGACCCCACTGCTGATGTTTCTG GACAGAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR217994 protein sequence
Red=Cloning site Green=Tags(s) MRRLICKRICDYKSFDDEESVDGNRPSSAASAFKVPAPKTPGNPVSSARKPGSAGGPKVGGPSKEGGAGA VDEDDFIKAFTDVPSVQIYSSRELEETLNKIREILSDDKHDWDQRANALKKIRSLLVAGAAQYDCFFQHL RLLDGALKLSAKDLRSQVVREACITVAHLSTVLGNKFDHGAEAIVPTLFNLVPNSAKVMATSGCAAIRFI IRHTHVPRLIPLITSNCTSKSVPVRRRSFEFLDLLLQEWQTHSLERHAAVLVETIKKGIHDADAEARVEA RKTYMGLRNHFPGEAETLYNSLEPSYQKSLQTYLKSSGSVASLPQSDRSSSSSQESLNRPFSSKWSTANP STVAGRVSVGGSKANPLPGSLQRSRSDIDVNAAAGAKAHHAAGQAVRSGRLGAGALNPGSYASLEDTSDK MDGTASDDGRVRAKLSTPLVAVGNAKTDSRGRSRTKMVSQSQPGSRSGSPGRVLTTTALSTVSSGAQRVL VNSASAQKRSKIPRSQGCSREASPSRLSVARSSRIPRPSVSQGCSREASRESSRDTSPVRSFQPLGPGYG ISQSSRLSSSVSAMRVLNTGSDVEEAVADALLLGDIRTKKKPARRRYESYGMHSDDDANSDASSACSERS YSSRNGSIPTYMRQTEDVAEVLNRCASSNWSERKEGLLGLQNLLKNQRTLSRVELKRLCEIFTRMFADPH GKVFSMFLETLVDFIQVHKDDLQDWLFVLLTQLLKKMGADLLGSVQAKVQKALDITRESFPNDLQFNILM RFTVDQTQTPSLKVKVAILKYIETLAKQMDPRDFTNSSETRLAVSRVITWTTEPKSSDVRKAAQSVLISL FELNTPEFTMLLGALPKTFQDGATKLLHNHLRNTGNGTQSSMGSPLTRPTPRSPANWSSPLTSPTNTSQN TLSPSAFDYDTENMNSEDIYSSLRGVTEAIQNFSFRSQEDMSEPVRRDPKKEDGDTICSGPGMSDPRAGG DAADGSQPALDNKASLLHSMPLHSSPRSRDYNPYNYSDSISPFNKSALKEAMFDDDADQFPDDLSLDHSD LVAELLKELSNHNERIEERKIALYELMKLTQEESFSVWDEHFKTILLLLLETLGDKEPTIRALALKVLKE ILRHQPARFKNYAELTVMKTLEAHKDPHKEVVRSAEEAASVLATSISPEQCIKVLCPIIQTADYPINLAA IKMQTKVIERVSKETLNMLLPEIMPGLIQGYDNSESSVRKACVFCLVAVHAVIGDELKPHLSQLTGSKMK LLNLYIKRAQTGSAGADPTADVSGQS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_029633 |
| ORF Size | 3858 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_029633.2, NP_083909.2 |
| RefSeq Size | 5624 bp |
| RefSeq ORF | 3861 bp |
| Locus ID | 76499 |
| MW | 140.7 kDa |
| Gene Summary | Microtubule plus-end tracking protein that promotes the stabilization of dynamic microtubules. Involved in the nucleation of noncentrosomal microtubules originating from the trans-Golgi network (TGN). Required for the polarization of the cytoplasmic microtubule arrays in migrating cells towards the leading edge of the cell. May act at the cell cortex to enhance the frequency of rescue of depolymerizing microtubules by attaching their plus-ends to cortical platforms composed of ERC1 and PHLDB2. This cortical microtubule stabilizing activity is regulated at least in part by phosphatidylinositol 3-kinase signaling. Also performs a similar stabilizing function at the kinetochore which is essential for the bipolar alignment of chromosomes on the mitotic spindle. Acts as a mediator of ERBB2-dependent stabilization of microtubules at the cell cortex.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224148 | Clasp2 (untagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1, (10ug) |
CNY 16,530.00 |
|
| MG217994 | Clasp2 (tGFP-tagged) - Mouse CLIP associating protein 2 (Clasp2) transcript variant 1, (10ug) |
CNY 14,160.00 |
|
| MR217994L3 | Lenti ORF clone of Clasp2 (Myc-DDK-tagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1 |
CNY 14,730.00 |
|
| MR217994L4 | Lenti ORF clone of Clasp2 (mGFP-tagged) - Mouse CLIP associating protein 2 (Clasp2), transcript variant 1 |
CNY 14,730.00 |
