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C5ar2 (NM_001146005) Mouse Tagged ORF Clone
CAT＃: MR218555
- TrueORF®
C5ar2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 2
ORF Plasmid: tGFP
"NM_001146005" in other vectors (4)
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CNY 2,850.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | C5L2; E030029A11Rik; Gpr77 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR218555 representing NM_001146005
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGAGGCAGAGAACAGCGCAGCCGGTGTCTGGGGGCCAGAATGATGAACCACACCACCAGCGAGTATT ATGACTATGAGTATGACCATGAACACTACAGTGACCTTCCGGACGTGCCCGTAGACTGCCCAGCTGGCAC CTGCTTCACTAGTGATGTCTACCTGATAGTCCTGCTTGTGCTGTATGCAGCCGTCTTCCTGGTGGGTGTG CCAGGCAACACCCTGGTGGCGTGGGTGACCTGGAAAGAGTCCCGCCACAGGCTTGGGGCCTCTTGGTTCC TGCACCTGACCATGGCCGACTTGCTTTGTTGTGTGTCTCTGCCCTTCCTGGCTGTGCCCATCGCCCAAAA GGGCCACTGGCCATACGGGGCGGCAGGCTGCTGGCTGTTGTCCTCCATCACCATTCTGTCTATGTATGCC AGTGTGCTGCTCCTGACCGGCCTCAGCGGAGACCTCTTCCTACTGGCTTTCAGGCCCTCCTGGAAGGGTG CAGATCATCGAACATTTGGGGTGCGTGTGGTCCAGGCCTCCTCCTGGATGCTGGGCCTCTTGCTGACTGT GCCTTCTGCTGTCTACCGTAGGCTGCTTCAGGAGCACTATCCTCCCCGACTTGTGTGTGGCATAGACTAT GGGGGATCGGTCAGCGCCGAGGTTGCCATCACCACTGTTCGATTCCTCTTTGGCTTCCTGGGGCCATTGG TGTTCATGGCCGGTTGTCACGGCATCCTCCAACGGCAGATGGCCCGCCGCCATTGGCCCCTGGGCACAGC TGTCGTGGTGGGGTTTTTCATTTGCTGGACCCCTTATCACGTCCTGCGGGTAATCATTGCAGCGGCTCCT CCGCACTCCTTGCTCCTCGCCCGGGTCCTGGAGGCGGAGCCTCTGTTTAATGGCCTGGCCCTGGCTCACA GTGCTCTCAATCCCATAATGTTTTTGTATTTCGGAAGAAAACAACTCTGCAAGTCACTCCAGGCTGCATG CCACTGGGCCCTGAGGGATCCACAGGATGAGGAAAGTGCGGTGACCAAGGTATCCATATCCACCAGCCAC GAAATGGTGTCTGAGATGCCGGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR218555 representing NM_001146005
Red=Cloning site Green=Tags(s) MRGREQRSRCLGARMMNHTTSEYYDYEYDHEHYSDLPDVPVDCPAGTCFTSDVYLIVLLVLYAAVFLVGV PGNTLVAWVTWKESRHRLGASWFLHLTMADLLCCVSLPFLAVPIAQKGHWPYGAAGCWLLSSITILSMYA SVLLLTGLSGDLFLLAFRPSWKGADHRTFGVRVVQASSWMLGLLLTVPSAVYRRLLQEHYPPRLVCGIDY GGSVSAEVAITTVRFLFGFLGPLVFMAGCHGILQRQMARRHWPLGTAVVVGFFICWTPYHVLRVIIAAAP PHSLLLARVLEAEPLFNGLALAHSALNPIMFLYFGRKQLCKSLQAACHWALRDPQDEESAVTKVSISTSH EMVSEMPV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001146005 |
| ORF Size | 1074 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001146005.1, NP_001139477.1 |
| RefSeq Size | 3429 bp |
| RefSeq ORF | 1077 bp |
| Locus ID | 319430 |
| UniProt ID | Q8BW93 |
| MW | 40.3 kDa |
| Gene Summary | Receptor for the chemotactic and inflammatory C3a, C4a and C5a anaphylatoxin peptides and also for their dearginated forms ASP/C3adesArg, C4adesArg and C5adesArg respectively. Couples weakly to G(i)-mediated signaling pathways (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC214409 | C5ar2 (untagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 2, (10ug) |
CNY 3,990.00 |
|
| MG218555 | C5ar2 (tGFP-tagged) - Mouse G protein-coupled receptor 77 (Gpr77) transcript variant 2, (10ug) |
CNY 3,140.00 |
|
| MR218555L3 | Lenti ORF clone of C5ar2 (Myc-DDK-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 2 |
CNY 4,750.00 |
|
| MR218555L4 | Lenti ORF clone of C5ar2 (mGFP-tagged) - Mouse G protein-coupled receptor 77 (Gpr77), transcript variant 2 |
CNY 4,750.00 |
