United States
Germany
Japan
United Kingdom
China
Syvn1 (NM_001164709) Mouse Tagged ORF Clone
CAT＃: MR219322
- TrueORF®
Syvn1 (Myc-DDK-tagged) - Mouse synovial apoptosis inhibitor 1, synoviolin (Syvn1), transcript variant 2
ORF Plasmid: tGFP
"NM_001164709" in other vectors (3)
Need custom modification / cloning service?
Get a free quote
CNY 6,080.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 1200010C09Rik; AW211966; C85322; D530017H19Rik; Hrd1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR219322 representing NM_001164709
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTTCCGCACCGCAGTGATGATGGCGGCCAGCCTGGCGCTAACCGGGGCAGTGGTGGCTCATGCCTACT ACCTCAAACACCAGTTCTACCCCACTGTAGTGTATTTGACCAAGTCCAGCCCCAGCATGGCAGTCCTGTA CATCCAGGCCTTTGTCCTTGTCTTCCTCTTGGGCAAGGTGATGGGCAAGGTGTTCTTCGGGCAGCTGAGG GCAGCAGAGATGGAGCACCTTCTGGAACGGTCCTGGTACGCTGTTACTGAGACTTGTTTGGCCTTCACCG TTTTTCGGGATGACTTCAGCCCTCGCTTTGTGGCGCTCTTTACGCTGCTCCTCTTCCTCAAATGTTTCCA TTGGTTGGCTGAAGACCGTGTGGACTTTATGGAACGCAGCCCCAACATCTCCTGGCTCTTCCACTGCCGC ATCGTCTCTCTCATGTTTCTCCTGGGTATCCTGGACTTCCTCTTCGTCAGCCACGCTTATCACAGCATCC TGACCCGTGGGGCTTCTGTGCAGCTGGTATTTGGCTTTGAGTACGCCATTCTGATGACCATGGTGCTTAC CATCTTCATCAAGTATGTGCTGCACTCCGTGGACCTCCAGAGCGAGAACCCCTGGGACAACAAGGCTGTA TACATGCTCTACACGGAGCTGTTTACAGGCTTCATCAAGGTCCTGCTGTACATGGCCTTCATGACCATCA TGATCAAGGTGCACACATTCCCACTCTTTGCCATTAGGCCCATGTACCTGGCCATGAGGCAGTTCAAGAA AGCTGTGACAGATGCCATCATGTCTCGCCGAGCCATCCGCAACATGAACACACTGTACCCAGATGCCACC CCCGAGGAGCTCCAGGCAATGGATAATGTCTGCATCATCTGCAGAGAAGAAATGGTGACTGGTGCTAAGA GATTGCCTTGCAACCACATCTTTCACACGAGCTGCCTGCGCTCCTGGTTCCAGAGACAGCAGACCTGCCC GACATGCCGCATGGATGTCCTGCGGGCATCGTTGCCAGCCCAGTCACCACCACCTCCTGAGCCTGCTGAC CAAGGACCACCCCCCGCCCCTCATCCCCAACCGCTGCTGCCACAGCCCCCTAATTTCCCCCAGGGCCTCC TGCCTCCTTTTCCTCCAGGCATGTTCCCACTGTGGCCCCCAATGGGTCCCTTTCCACCTGTCCCGCCTCC CCCAAGCTCAGGAGAGGCTGCGGCCCCTCCACCCACCAGTACAGCCGTTTCTCGGCCTAGTGGAGCAGCC ACCACCACAGCTGCTGGCACCAGTACTTCTGCCCCAGCACCTGGGTCTGTACCTGGCCCAGAGGCTGGTC CTGCCCCCGGCTTCCCTTTCCCTCCTCCTTGGATGGGTATGCCTCTGCCTCCACCTTTTGCCTTCCCCCC AATGCCTGTGCCCCCTGCGGGCTTTGCTGGCCTAACCCCAGAGGAGCTGCGAGCACTAGAGGGCCATGAG CGGCAGCACCTGGAGGCCCGGCTGCAGAGTCTGCGCAACATACACACACTACTGGATGCTGCCATGCTTC AAATCAACCAGTACCTCACTGTGCTGGCTTCCCTGGGGCCCCCCAGGCCAGCTACTTCAGTGAACCCCAC TGAAGAGACTGCCTCTACAGTGGTATCTGCTGCCCCTTCCACCAGCGCCCCCAGCTCTGAGGCTCCTACC CCGTCTCCGGGAGCTTCCCCACCAATTCCTGAAGCAGAAAAGCCTCCTGCTCCTGAGTCAGTGGGCATTG TAGAGGAGCTTCCCGAGGATGGAGAGCCTGATGCTGCAGAACTCCGCCGGCGTCGCCTGCAGAAGCTGGA GTCCCCTGTTGCCCAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR219322 representing NM_001164709
Red=Cloning site Green=Tags(s) MFRTAVMMAASLALTGAVVAHAYYLKHQFYPTVVYLTKSSPSMAVLYIQAFVLVFLLGKVMGKVFFGQLR AAEMEHLLERSWYAVTETCLAFTVFRDDFSPRFVALFTLLLFLKCFHWLAEDRVDFMERSPNISWLFHCR IVSLMFLLGILDFLFVSHAYHSILTRGASVQLVFGFEYAILMTMVLTIFIKYVLHSVDLQSENPWDNKAV YMLYTELFTGFIKVLLYMAFMTIMIKVHTFPLFAIRPMYLAMRQFKKAVTDAIMSRRAIRNMNTLYPDAT PEELQAMDNVCIICREEMVTGAKRLPCNHIFHTSCLRSWFQRQQTCPTCRMDVLRASLPAQSPPPPEPAD QGPPPAPHPQPLLPQPPNFPQGLLPPFPPGMFPLWPPMGPFPPVPPPPSSGEAAAPPPTSTAVSRPSGAA TTTAAGTSTSAPAPGSVPGPEAGPAPGFPFPPPWMGMPLPPPFAFPPMPVPPAGFAGLTPEELRALEGHE RQHLEARLQSLRNIHTLLDAAMLQINQYLTVLASLGPPRPATSVNPTEETASTVVSAAPSTSAPSSEAPT PSPGASPPIPEAEKPPAPESVGIVEELPEDGEPDAAELRRRRLQKLESPVAH TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001164709 |
| ORF Size | 1836 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001164709.1, NP_001158181.1 |
| RefSeq Size | 3103 bp |
| RefSeq ORF | 1839 bp |
| Locus ID | 74126 |
| UniProt ID | Q9DBY1 |
| MW | 67.8 kDa |
| Gene Summary | Acts as an E3 ubiquitin-protein ligase which accepts ubiquitin specifically from endoplasmic reticulum-associated UBC7 E2 ligase and transfers it to substrates, promoting their degradation (PubMed:12975321, PubMed:15611074). Component of the endoplasmic reticulum quality control (ERQC) system also called ER-associated degradation (ERAD) involved in ubiquitin-dependent degradation of misfolded endoplasmic reticulum proteins (PubMed:12975321, PubMed:15611074). Also promotes the degradation of normal but naturally short-lived proteins such as SGK. Protects cells from ER stress-induced apoptosis. Sequesters p53/TP53 in the cytoplasm and promotes its degradation, thereby negatively regulating its biological function in transcription, cell cycle regulation and apoptosis (PubMed:17170702). Required for embryogenesis (PubMed:15611074). Mediates the ubiquitination and subsequent degradation of cytoplasmic NFE2L1 (PubMed:21911472).[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Estrogens Promote Misfolded Proinsulin Degradation to Protect Insulin Production and Delay Diabetes
,Xu, B;Allard, C;Alvarez-Mercado, AI;Fuselier, T;Kim, JH;Coons, LA;Hewitt, SC;Urano, F;Korach, KS;Levin, ER;Arvan, P;Floyd, ZE;Mauvais-Jarvis, F;,
Cell Rep
,PubMed ID 29972779
[SYVN1]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG219322 | Syvn1 (tGFP-tagged) - Mouse synovial apoptosis inhibitor 1 synoviolin (Syvn1) transcript variant 2, (10ug) |
CNY 6,650.00 |
|
| MR219322L3 | Lenti ORF clone of Syvn1 (Myc-DDK-tagged) - Mouse synovial apoptosis inhibitor 1, synoviolin (Syvn1), transcript variant 2 |
CNY 7,980.00 |
|
| MR219322L4 | Lenti ORF clone of Syvn1 (mGFP-tagged) - Mouse synovial apoptosis inhibitor 1, synoviolin (Syvn1), transcript variant 2 |
CNY 7,980.00 |
