Rab34 (NM_033475) Mouse Tagged ORF Clone

CAT#: MR220587

  • TrueORF®

Rab34 (Myc-DDK-tagged) - Mouse RAB34, member of RAS oncogene family (Rab34), transcript variant 1

ORF Plasmid: DDK tGFP



  "NM_033475" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 2,081.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Mouse Tagged ORF Clone
Tag Myc-DDK
Synonyms AI326479; Narr; Rah; Rah1
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>MR220587 representing NM_033475
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAACATTCTGGCGCCCGTGCGGAGGGACCGCGTCCTGGCGGAGCTGCCCCAGTGCCTGAAGAAAGAGG
CCGCTTTGCACGTGCGCAAAGACTTCCACCCCCGCGTCACTTGCGCCTGCCAGGAGCACCGGACAGGCAC
CGTGGGATTTAAGATATCCAAGGTCATCGTTGTGGGAGACCTATCTGTGGGGAAGACCTGTCTCATTAAT
AGGTTCTGCAAAGACACCTTCGATAAGAATTACAAGGCTACCATCGGAGTGGATTTTGAGATGGAACGAT
TTGAAGTCTTGGGTGTCCCCTTCAGTCTCCAACTTTGGGACACGGCTGGTCAGGAAAGGTTCAAGTGCAT
TGCTTCCACCTACTACCGTGGAGCTCAAGCCATCATCATTGTCTTCAACCTGAATGACGTGGCATCCCTG
GAACACACCAAGCAGTGGCTCACTGATGCCCTCAAGGAGAACGACCCTTCCAACGTGCTCCTCTTCCTTG
TGGGTTCCAAGAAGGACCTGAGTACTCCTGCTCAGTATTCCCTAATGGAGAAGGATGCCCTCAAGGTGGC
CCAAGAGATTAAGGCCGAGTACTGGGCGGTGTCCTCCCTCACTGGTGAGAATGTCCGGGAATTCTTCTTC
CGTGTGGCAGCACTAACCTTTGAGGCCAATGTCCTGGCGGAGCTGGAGAAATCCGGGGCTCGGCACATTG
CAGATGTTGTTCGCATCAACAGCGATGACAAAAACCTGTACCTAACTGCCAGCAAGAAAAAGGCCACATG
TTGTCCC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>MR220587 representing NM_033475
Red=Cloning site Green=Tags(s)

MNILAPVRRDRVLAELPQCLKKEAALHVRKDFHPRVTCACQEHRTGTVGFKISKVIVVGDLSVGKTCLIN
RFCKDTFDKNYKATIGVDFEMERFEVLGVPFSLQLWDTAGQERFKCIASTYYRGAQAIIIVFNLNDVASL
EHTKQWLTDALKENDPSNVLLFLVGSKKDLSTPAQYSLMEKDALKVAQEIKAEYWAVSSLTGENVREFFF
RVAALTFEANVLAELEKSGARHIADVVRINSDDKNLYLTASKKKATCCP

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_033475
ORF Size 777 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_033475.3, NP_258436.2
RefSeq Size 1721 bp
RefSeq ORF 780 bp
Locus ID 19376
UniProt ID Q64008
MW 29.1 kDa
Gene Summary Protein transport. Plays a role in the maturation of phagosomes that engulf pathogens, such as S.aureus and Mycobacterium. Plays a role in the fusion of phagosomes with lysosomes (By similarity). Involved in the redistribution of lysosomes to the peri-Golgi region (PubMed:12475955). Acts also as a positive regulator of hedgehog signaling and regulates ciliary function (PubMed:29290584).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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