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Pias3 (NM_018812) Mouse Tagged ORF Clone
CAT＃: MR220934
- TrueORF®
Pias3 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 2
ORF Plasmid: tGFP
"NM_018812" in other vectors (4)
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CNY 5,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Pias3l |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR220934 representing NM_018812
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGAGCTGGGCGAGTTAAAGCACATGGTGATGAGTTTCCGAGTGTCTGAGCTCCAGGTGCTCCTCG GCTTCGCTGGCAGGAACAAGAGTGGGCGGAAACACGAGCTGCTGGCCAAGGCCCTGCACCTCCTCAAGTC TAGCTGCGCCCCCAGCGTCCAGATGAAGATCAAAGAACTTTATCGCAGGCGCTTTCCCCGGAAGACCCTG GGGCCTTCTGATCTCTCCTTGCTTTCTTTGCCCCCTGGCACCTCTCCTCCTGTGCACCCCGATGTCACCA TGAAGCCACTGCCCTTCTATGAAGTCTATGGGGAGCTCATCCGACCCACCACCCTTGCGTCCACCTCCAG CCAGAGGTTCGAGGAAGCCCACTTCACCTTCGCGCTCACTCCCCAGCAGCTGCAGCAGATTCTCACGTCC AGGGAGGTTCTGCCAGGAGCCAAGTGTGATTACACCATACAAGTGCAGCTCAGATTCTGTCTCTGTGAGA CCAGCTGCCCTCAGGAGGACTATTTCCCCCCTAACCTCTTTGTTAAGGTTAATGGGAAACTCTGCCCCCT GCCGGGTTACCTCCCTCCAACCAAGAATGGAGCTGAGCCCAAGAGGCCCAGCCGTCCGATCAACATCACA CCCTTGGCTCGACTCTCAGCCACTGTCCCCAACACCATCGTAGTTAATTGGTCATCTGAGTTTGGACGGA ATTACTCCTTGTCCGTGTACCTGGTGAGGCAATTGACTGCAGGGACCCTTCTACAAAAACTCAGAGCCAA GGGGATCCGGAATCCAGACCATTCCCGGGCACTGATCAAGGAGAAACTGACTGCTGACCCCGACAGTGAA GTGGCTACTACAAGTCTCCGGGTGTCACTCATGTGCCCGCTAGGGAAGATGCGCCTGACTGTCCCGTGTC GTGCCCTCACCTGTGCCCATCTGCAGAGTTTCGATGCTGCCCTTTATCTACAGATGAATGAGAAGAAGCC GACATGGACGTGTCCTGTGTGTGACAAGAAGGCTCCCTATGAGTCGCTGATTATTGATGGTTTATTCATG GAAATTCTTAATTCCTGTTCGGATTGTGATGAGATCCAGTTCATGGAAGATGGATCCTGGTGTCCGATGA AACCCAAGAAGGAGGCATCAGAGGTTTGCCCCCCGCCAGGGTATGGGCTGGATGGTCTCCAGTACAGCGC AGTCCAGGAGGGAATTCAGCCAGAGAGTAAGAAGAGGGTCGAAGTCATTGACTTGACCATCGAAAGCTCA TCAGATGAGGAGGATTTGCCCCCCACCAAGAAGCACTGCCCTGTCACCTCAGCGGCCATTCCAGCCCTTC CTGGAAGCAAAGGAGCCCTGACCTCTGGTCACCAGCCATCCTCGGTGCTGCGGAGCCCTGCAATGGGCAC ACTGGGCAGTGACTTCCTGTCTAGTCTCCCGCTACATGAGTACCCACCTGCCTTCCCACTGGGGGCTGAC ATCCAAGGTTTAGATTTATTTTCTTTCCTTCAGACTGAGAGTCAGCACTACGGCCCTTCAGTTATCACTT CGCTAGATGAACAGGACACCCTTGGCCACTTCTTCCAGTACCGGGGAACCCCTTCCCACTTCCTGGGCCC ACTGGCCCCCACACTGGGGAGCTCTCACCGCAGCTCCACTCCAGCGCCCCCTCCTGGTCGTGTCAGCAGC ATTGTGGCTCCTGGGAGCTCCTTGAGGGAAGGGCATGGAGGACCCCTGCCTTCAGGTCCCTCTTTGACTG GCTGTCGGTCAGACGTCATTTCCTTGGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR220934 representing NM_018812
Red=Cloning site Green=Tags(s) MAELGELKHMVMSFRVSELQVLLGFAGRNKSGRKHELLAKALHLLKSSCAPSVQMKIKELYRRRFPRKTL GPSDLSLLSLPPGTSPPVHPDVTMKPLPFYEVYGELIRPTTLASTSSQRFEEAHFTFALTPQQLQQILTS REVLPGAKCDYTIQVQLRFCLCETSCPQEDYFPPNLFVKVNGKLCPLPGYLPPTKNGAEPKRPSRPINIT PLARLSATVPNTIVVNWSSEFGRNYSLSVYLVRQLTAGTLLQKLRAKGIRNPDHSRALIKEKLTADPDSE VATTSLRVSLMCPLGKMRLTVPCRALTCAHLQSFDAALYLQMNEKKPTWTCPVCDKKAPYESLIIDGLFM EILNSCSDCDEIQFMEDGSWCPMKPKKEASEVCPPPGYGLDGLQYSAVQEGIQPESKKRVEVIDLTIESS SDEEDLPPTKKHCPVTSAAIPALPGSKGALTSGHQPSSVLRSPAMGTLGSDFLSSLPLHEYPPAFPLGAD IQGLDLFSFLQTESQHYGPSVITSLDEQDTLGHFFQYRGTPSHFLGPLAPTLGSSHRSSTPAPPPGRVSS IVAPGSSLREGHGGPLPSGPSLTGCRSDVISLD TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018812 |
| ORF Size | 1779 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018812.2, NP_061282.2 |
| RefSeq Size | 2833 bp |
| RefSeq ORF | 1782 bp |
| Locus ID | 229615 |
| UniProt ID | O54714 |
| MW | 65.2 kDa |
| Gene Summary | Functions as an E3-type small ubiquitin-like modifier (SUMO) ligase, stabilizing the interaction between UBE2I and the substrate, and as a SUMO-tethering factor. Plays a crucial role as a transcriptional coregulation in various cellular pathways, including the STAT pathway and the steroid hormone signaling pathway. Repressor of STAT3 signaling via inhibiting STAT3 DNA-binding and suppressing cell growth. Repressor of MITF transcriptional activity. Enhances the sumoylation of MTA1 and may participate in its paralog-selective sumoylation. Sumoylates CCAR2 which promotes its interaction with SIRT1 (By similarity). Diminishes the sumoylation of ZFHX3 by preventing the colocalization of ZFHX3 with SUMO1 in the nucleus (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC219462 | Pias3 (untagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 2, (10ug) |
CNY 7,510.00 |
|
| MG220934 | Pias3 (tGFP-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3) transcript variant 2, (10ug) |
CNY 6,460.00 |
|
| MR220934L3 | Lenti ORF clone of Pias3 (Myc-DDK-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 2 |
CNY 7,790.00 |
|
| MR220934L4 | Lenti ORF clone of Pias3 (mGFP-tagged) - Mouse protein inhibitor of activated STAT 3 (Pias3), transcript variant 2 |
CNY 7,790.00 |
