Pfkm (NM_001163487) Mouse Tagged ORF Clone

CAT＃: MR221897

TrueORF^®

Pfkm (Myc-DDK-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 1

ORF Plasmid: DDK tGFP

Datasheet

SDS

Plasmid Map

"NM_001163487" in other vectors (4)

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Mouse Protein / Lysate Request

CNY 5,720.00

CNY 5,800.00

货期*

现货

规格

Product images

经常一起买 (4)

PS100001

pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug

CNY 5,420.00

CC100003

DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)

CNY 1,280.00

PRIMER

Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each

CNY 480.00

TA592569S

DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags

CNY 300.00

【特别福利】购一抗正装产品，免费领10ul抗体试用装

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI131669; ATP-PFK; Pfk-4; PFK-A; PFK-M; Pfk4; Pfka; Pfkx
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR221897 ORF sequence Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCCATGAAGAGCATCATGCAGCCAAAACCCTGGGGATCGGCAAGGCCATCGCCGTGTTGACCTCTG GTGGAGATGCCCAAGGTATGAATGCTGCCGTCAGGGCTGTGGTCCGAGTTGGTATCTTCACGGGCGCCCG CGTCTTCTTTGTCCATGAGGGTTACCAAGGCCTGGTGGACGGTGGCGAGCACATCAGGGAGGCCACGTGG GAGAGCGTGTCTATGATGCTTCAGCTGGGTGGCACAGTGATTGGAAGTGCCCGATGCAAGGACTTCCGGG AGCGAGAAGGACGACTCCGGGCCGCCCACAACCTGGTGAAGCGGGGGATCACCAATCTGTGTGTCATCGG AGGCGATGGCAGCCTCACTGGGGCTGACACTTTCCGTTCAGAGTGGAGCGACTTGCTGAATGATTTCCAG AAAGACGGGAAGATCACAGCCGAGGAGGCTACAAAGTCCAGCTACCTGAACATCGTGGGCCTGGTTGGCT CAATCGACAATGACTTCTGTGGCACTGATATGACCATTGGTACTGACTCTGCCCTGCACAGGATTGTGGA GATCGTAGACGCCATCACCACCACGGCTCAGAGCCACCAGAGGACGTTTGTGTTAGAAGTGATGGGCCGC CACTGTGGATACCTGGCCCTTGTCACCTCTCTGTCCTGTGGGGCCGACTGGGTTTTTATTCCTGAGTGTC CGCCAGACGATGACTGGGAAGAACACCTTTGTCGCCGGCTCAGTGAGACAAGGACCCGTGGCTCTCGTCT CAACATCATCATTGTTGCTGAAGGTGCCATCGACAAGAACGGGAAGCCAATCACCTCAGAAGACATCAAG AACCTGGTGGTGAAGCGCCTTGGATATGACACCCGGGTCACTGTTCTGGGACATGTACAGCGGGGTGGGA CACCATCAGCCTTTGACCGGATCCTGGGCAGCAGGATGGGTGTGGAAGCAGTGATGGCACTTTTGGAGGG GACCCCAGACACCCCAGCCTGTGTGGTGAGCCTCTCTGGTAACCAGGCTGTGCGTTTGCCCCTCATGGAG TGCGTGCAGGTGACCAAAGACGTGACCAAGGCTATGGATGAGAAGAGATTTGATGAAGCCATTAAGCTGA GAGGCCGGAGCTTCATGAACAACTGGGAGGTATACAAGCTTCTAGCTCATGTCAGACCCCCAGTCTCTAA GGGTGGGTTGCACACAGTGGCCGTGATGAATGTGGGGGCCCCAGCTGCTGGCATGAATGCCGCTGTTCGC TCTACCGTGAGGATTGGCCTTATCCAGGGCAACCGGGTGCTGGTCGTACATGATGGCTTTGAGGGTCTGG CCAAAGGTCAGATTGAGGAGGCTGGCTGGAGCTATGTTGGAGGCTGGACTGGTCAAGGTGGTTCCAAACT TGGTACTAAAAGGACTCTCCCCAAGAAGAACCTGGAACAGATCAGTGCCAACATAACCAAGTTTAACATC CAGGGCCTTGTCATCATTGGGGGCTTTGAGGCTTACACAGGGGGCTTGGAGCTGATGGAAGGCAGGAAGC AGTTTGATGAGCTCTGCATCCCGTTTGTGGTCATTCCGGCCACGGTTTCCAATAACGTCCCTGGGTCAGA CTTCAGCATCGGGGCTGACACAGCACTGAACACCATCTGCACGACTTGTGACCGAATCAAGCAGTCTGCA GCAGGCACCAAACGTCGGGTGTTTATCATCGAGACTATGGGTGGCTACTGTGGCTACCTGGCCACCATGG CAGGGCTGGCCGCTGGGGCTGATGCTGCCTACATTTTTGAGGAACCCTTCACCATTCGCGATCTCCAGGT GAATGTTGAACATCTGGTGCAGAAGATGAAAACAACTGTGAAGAGAGGCCTGGTGCTGAGGAATGAGAAA TGCAATGAGAACTACACTACTGACTTCATTTTCAACCTGTACTCTGAGGAGGGGAAGGGCATCTTTGACA GCAGGAAGAACGTGCTTGGCCACATGCAGCAGGGTGGAAGCCCAACTCCCTTTGACAGGAATTTTGCCAC TAAGATGGGTGCTAAGGCTATGAACTGGATGTCTGGGAAAATCAAGGAGAGTTACCGTAATGGACGGATC TTTGCCAACACCCCTGACTCAGGCTGCGTTCTGGGGATGCGTAAGAGGGCCCTGGTCTTTCAGCCAGTAA CTGAGCTGAAGGACCAGACAGACTTTGAACACCGAATCCCCAAAGAACAGTGGTGGCTGAAGCTGAGGCC AATCCTCAAAATCCTAGCCAAGTACGAGATTGATCTGGACACCTCCGACCACGCCCACCTGGAGCACATT TCAAGGAAACGGTCTGGAGAAGCCGCCGTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR221897 protein sequence Red=Cloning site Green=Tags(s) MTHEEHHAAKTLGIGKAIAVLTSGGDAQGMNAAVRAVVRVGIFTGARVFFVHEGYQGLVDGGEHIREATW ESVSMMLQLGGTVIGSARCKDFREREGRLRAAHNLVKRGITNLCVIGGDGSLTGADTFRSEWSDLLNDFQ KDGKITAEEATKSSYLNIVGLVGSIDNDFCGTDMTIGTDSALHRIVEIVDAITTTAQSHQRTFVLEVMGR HCGYLALVTSLSCGADWVFIPECPPDDDWEEHLCRRLSETRTRGSRLNIIIVAEGAIDKNGKPITSEDIK NLVVKRLGYDTRVTVLGHVQRGGTPSAFDRILGSRMGVEAVMALLEGTPDTPACVVSLSGNQAVRLPLME CVQVTKDVTKAMDEKRFDEAIKLRGRSFMNNWEVYKLLAHVRPPVSKGGLHTVAVMNVGAPAAGMNAAVR STVRIGLIQGNRVLVVHDGFEGLAKGQIEEAGWSYVGGWTGQGGSKLGTKRTLPKKNLEQISANITKFNI QGLVIIGGFEAYTGGLELMEGRKQFDELCIPFVVIPATVSNNVPGSDFSIGADTALNTICTTCDRIKQSA AGTKRRVFIIETMGGYCGYLATMAGLAAGADAAYIFEEPFTIRDLQVNVEHLVQKMKTTVKRGLVLRNEK CNENYTTDFIFNLYSEEGKGIFDSRKNVLGHMQQGGSPTPFDRNFATKMGAKAMNWMSGKIKESYRNGRI FANTPDSGCVLGMRKRALVFQPVTELKDQTDFEHRIPKEQWWLKLRPILKILAKYEIDLDTSDHAHLEHI SRKRSGEAAV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI Cloning Scheme for this gene Plasmid Map
ACCN	NM_001163487
ORF Size	2343 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001163487.1, NP_001156959.1
RefSeq Size	2894 bp
RefSeq ORF	2343 bp
Locus ID	18642
UniProt ID	P47857
MW	85.3 kDa
Gene Summary	Catalyzes the phosphorylation of D-fructose 6-phosphate to fructose 1,6-bisphosphate by ATP, the first committing step of glycolysis.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

Documents

Product Manuals
TrueORFs: Open Reading Frame Clones - 10 ug in PrecisionShuttle Vector System pLenti-ORF destination vector Transfection - TurboFectin 8.0 Transfection Reagent Plasmid Preparation - PowerPrep Plasmid Purification Kits
FAQs
TrueORF Clones/PrecisionShuttle Organelle Marker Transfection Reagent
SDS
TrueORF SDS

Resources

cDNA 克隆相关资源
Video Tutorial: Clone Selection Guide Product Video: What is TrueORF GOLD and why it is the most popular type of clone Video Tutorial: How to make Lentivirus particles Video Tutorial: Is the Lentivirus Safe Product Brochure: cDNA clones $98 Clones. Download the List of 15,000 Protocol: Lentivirus packaging

Other Versions

SKU	Description	Size	Price
MC221559	Pfkm (untagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 1, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 10,070.00
MG221897	Pfkm (tGFP-tagged) - Mouse phosphofructokinase muscle (Pfkm) transcript variant 1, (10ug)	10 ug 产品选项验证失败所有选项都是必需的	CNY 6,370.00
MR221897L3	Lenti ORF clone of Pfkm (Myc-DDK-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00
MR221897L4	Lenti ORF clone of Pfkm (mGFP-tagged) - Mouse phosphofructokinase, muscle (Pfkm), transcript variant 1	10 ug 产品选项验证失败所有选项都是必需的	CNY 7,700.00

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