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Itga1 (NM_001033228) Mouse Tagged ORF Clone
CAT＃: MR222649
- TrueORF®
Itga1 (Myc-DDK-tagged) - Mouse integrin alpha 1 (Itga1)
ORF Plasmid: tGFP
"NM_001033228" in other vectors (4)
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CNY 8,208.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CD49A; E130012M19Rik; Vla1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222649 representing NM_001033228
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTCCCCAGGCGTCCTGCTAGCCTAGAGGTCACTGTAGCCTGCATCTGGCTTCTCACCGTTATCCTAG GCGTCTGCATCTCCTTCAACGTTGATGTCAAAAACTCCATGAGCTTCAGTGGTCCAGTGGAGGACATGTT TGGATACACCGTTCAACAGTATGAAAACGAAGAAGGCAAATGGGTTCTTATTGGTTCTCCTTTAGTTGGC CAACCCAAAGCAAGAACTGGAGATGTCTATAAGTGTCCAGTTGGGAGAGAGAGATCAATGCCTTGTGTGA AGTTGGATTTACCAGTTAACACATCGATTCCTAATGTCACAGAAATAAAGGAAAACATGACATTTGGATC AACTTTAGTCACCAATCCGAAGGGAGGATTTCTGGCATGTGGGCCCTTGTATGCCTATAGATGTGGACAT TTGCATTATACGACTGGCATATGTTCTGATGTCAGCCCTACATTTCAAGTTGTGAACTCGTTCGCCCCTG TACAAGAATGTAGCACACAGCTTGACATAGTCATCGTCCTAGATGGCTCCAACAGCATCTACCCCTGGGA AAGTGTCACTGCCTTTTTAAACGACCTTCTTAAGAGGATGGACATTGGCCCCAAACAGACACAGGTTGGA ATTGTACAATATGGAGCAAATGTAACACATGAGTTCAACCTCAATAAGTATTCATCCACAGAAGAGGTCC TGGTGGCAGCAAACAAAATAGGCCGCAGGGGTGGTCTCCAGACGATGACAGCCCTTGGAATAGACACAGC CAGGAAAGAGGCATTCACTGAAGCTCGGGGGGCCAGAAGGGGAGTGAAAAAAGTCATGGTTATTGTGACT GATGGAGAATCGCATGACAACTATCGCCTGAAACAGGTCATCCAAGACTGTGAGGATGAAAACATTCAGC GATTTTCCATAGCTATCCTTGGCCACTATAACAGGGGGAACTTAAGCACCGAAAAATTTGTGGAGGAGAT AAAATCAATCGCAAGTGAGCCCACCGAAAAGCACTTCTTCAACGTCTCAGATGAGTTGGCCCTGGTCACT ATTGTTAAAGCTCTGGGAGAAAGGATATTTGCTTTGGAAGCTACAGCTGACCAGTCAGCAGCTTCGTTTG AAATGGAGATGTCTCAGACCGGCTTCAGTGCTCATTATTCACAGGACTGGGTCATGCTTGGAGCTGTTGG AGCCTATGACTGGAACGGAACTGTGGTCATGCAGAAGGCTAACCAGATTGTCATCCCTCATAACACCACC TTTCAAACTGAGCCCACCAAGATGAACGAGCCTCTTGCTTCTTACTTGGGTTACACAGTGAACTCTGCCA CCATCCCGGGAGATGTGCTCTATATCGCTGGGCAGCCTCGGTACAATCACACCGGCCAGGTCGTCATCTA CAAGATGGAGGATGGGGACGTCAACATTCTGCAGACACTCAGTGGAGAGCAGATCGGATCCTACTTTGGT AGCGTCTTAACCACAATTGACATCGACAAAGATTCTTACACTGATCTGCTTCTCGTCGGGGCTCCCATGT ATATGGGGACAGAGAAAGAGGAACAGGGCAAGGTGTACGTGTACGCTGTGAATCAGACGAGGTTTGAATA TCAAATGAGCCTGGAACCAATTAAGCAGACCTGCTGTTCATCCCTGAAGGATAATTCATGCACAAAAGAA AACAAGAATGAGCCCTGTGGGGCCCGTTTTGGAACAGCAGTTGCTGCTGTAAAAGACCTCAACGTGGATG GCTTTAATGACGTCGTGATTGGAGCTCCCCTGGAAGACGACCACGCAGGAGCTGTGTACATTTATCATGG CAGTGGCAAGACCATAAGGAAAGAGTATGCGCAACGCATTCCCTCAGGTGGGGATGGGAAGACGCTGAAA TTTTTTGGCCAGTCTATCCACGGAGAGATGGATTTAAATGGCGATGGTCTGACTGACGTGACCATTGGAG GCCTTGGAGGAGCTGCCCTCTTCTGGGCCAGAGATGTGGCTGTAGTTAAAGTGACCATGAATTTTGAACC CAACAAAGTGAATATTCAAAAGAAAAACTGCCGTGTGGAGGGCAAAGAAACAGTATGCATAAATGCTACA ATGTGTTTTCATGTGAAATTGAAGTCTAAAGAAGACTCAGTTTACGAGGCTGATCTGCAGTACCGTGTCA CCCTTGATTCGCTGAGGCAGATATCACGGAGCTTTTTTTCTGGAACTCAGGAAAGGAGGATTCAAAGAAA CCTCACCGTTCGAGAATCCGAATGCATCAGGCACTCCTTCTACATGTTGGATAAGCACGACTTTCAGGAC TCCGTGAGAGTGACTTTGGATTTTAACCTCACTGATCCAGAAAATGGGCCCGTGCTCGATGACGCTCTGC CAAACTCAGTCCATGGACATATTCCTTTTGCCAAAGACTGTGGGAACAAGGAAAGATGCGTTTCAGACCT CACCCTGGATGTGTCCACAACAGAAAAGAACCTGCTGATTGTCAGATCCCAGAATGACAAGTTCAATGTC AGCCTCACCGTCAAAAACAAGGGAGACAGTGCGTACAACACCCGGACAGTGGTTCAGTATTCTCCAAATC TGATTTTTTCAGGAATTGAGGAGATCCAAAAAGATAGCTGCGAATCCAATCAAAATATCACGTGTAGAGT TGGATATCCTTTCCTGAGGACAGGAGACGTGGTTAACTTCAAAATAATATTCCAGTTTAATACATCACAT CTCTCAGAAAATGCAATTATTCATTTAAGTGCAACAAGTGACAGTGAAGAGCCCCTGGAATCTCTTTACG ATAATGAAGTAAATATTTCCATCCCCGTAAAATATGAAGTCGGACTGCAGTTTTACAGTTCTGCGAGTGA ACACCACATTTCAGTTGCTGCCAATGAGACTGTTCCTGAGCTGATTAATTCCACCAAGGACATTGGGGAT GAAATTAATGTCTTCTACACGATTAGAAAAAGAGGGCATTTCCCAATGCCAGAACTTCGGCTGGCAATTT CATTTCCCAATCTGACGTCAGATGGCTATCCTGTACTGTACCCAACTGGATGGTCATCTTCTGATAATGT AAACTGCAGACCTCGAAGCCTTGAGGACCCTTTGGGTATCAACTCTGGGAAGAAAATGACAATATCAAAG TCTGAGGTTCTCAAAAGAGGCACAATCCAGGACTGCAGTACCTGCAAGATTGCTACCATCACGTGTCATC TCCTTCCCTCGGATGTGAGTCAAGTGAATGTCTCACTCATCTTGTGGAAACCAACTTTCATAAAAGCACA TTTTTCCAGCTTAAATCTTACCATACGGGGAGAACTTCAGAGTGAAAACTCATCGCTGACTTTAAGCAGC AGCAACCGGAAACGAGAGCTGGCTATTCAGATATCCAAAGACGGGCTCCCAGGCCGAGTGCCGCTGTGGG TTATCCTCCTGAGCGCCTTTGCGGGACTCCTGCTGTTAATGCTGCTTATATTAGCTCTGTGGAAGATTGG ATTCTTCAAAAGGCCACTAAAGAAGAAAATGGAGAAA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222649 representing NM_001033228
Red=Cloning site Green=Tags(s) MVPRRPASLEVTVACIWLLTVILGVCISFNVDVKNSMSFSGPVEDMFGYTVQQYENEEGKWVLIGSPLVG QPKARTGDVYKCPVGRERSMPCVKLDLPVNTSIPNVTEIKENMTFGSTLVTNPKGGFLACGPLYAYRCGH LHYTTGICSDVSPTFQVVNSFAPVQECSTQLDIVIVLDGSNSIYPWESVTAFLNDLLKRMDIGPKQTQVG IVQYGANVTHEFNLNKYSSTEEVLVAANKIGRRGGLQTMTALGIDTARKEAFTEARGARRGVKKVMVIVT DGESHDNYRLKQVIQDCEDENIQRFSIAILGHYNRGNLSTEKFVEEIKSIASEPTEKHFFNVSDELALVT IVKALGERIFALEATADQSAASFEMEMSQTGFSAHYSQDWVMLGAVGAYDWNGTVVMQKANQIVIPHNTT FQTEPTKMNEPLASYLGYTVNSATIPGDVLYIAGQPRYNHTGQVVIYKMEDGDVNILQTLSGEQIGSYFG SVLTTIDIDKDSYTDLLLVGAPMYMGTEKEEQGKVYVYAVNQTRFEYQMSLEPIKQTCCSSLKDNSCTKE NKNEPCGARFGTAVAAVKDLNVDGFNDVVIGAPLEDDHAGAVYIYHGSGKTIRKEYAQRIPSGGDGKTLK FFGQSIHGEMDLNGDGLTDVTIGGLGGAALFWARDVAVVKVTMNFEPNKVNIQKKNCRVEGKETVCINAT MCFHVKLKSKEDSVYEADLQYRVTLDSLRQISRSFFSGTQERRIQRNLTVRESECIRHSFYMLDKHDFQD SVRVTLDFNLTDPENGPVLDDALPNSVHGHIPFAKDCGNKERCVSDLTLDVSTTEKNLLIVRSQNDKFNV SLTVKNKGDSAYNTRTVVQYSPNLIFSGIEEIQKDSCESNQNITCRVGYPFLRTGDVVNFKIIFQFNTSH LSENAIIHLSATSDSEEPLESLYDNEVNISIPVKYEVGLQFYSSASEHHISVAANETVPELINSTKDIGD EINVFYTIRKRGHFPMPELRLAISFPNLTSDGYPVLYPTGWSSSDNVNCRPRSLEDPLGINSGKKMTISK SEVLKRGTIQDCSTCKIATITCHLLPSDVSQVNVSLILWKPTFIKAHFSSLNLTIRGELQSENSSLTLSS SNRKRELAIQISKDGLPGRVPLWVILLSAFAGLLLLMLLILALWKIGFFKRPLKKKMEK TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001033228 |
| ORF Size | 3537 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001033228.3, NP_001028400.2 |
| RefSeq Size | 5984 bp |
| RefSeq ORF | 3540 bp |
| Locus ID | 109700 |
| UniProt ID | Q3V3R4 |
| MW | 131.3 kDa |
| Gene Summary | Integrin alpha-1/beta-1 is a receptor for laminin and collagen. It recognizes the proline-hydroxylated sequence G-F-P-G-E-R in collagen. Involved in anchorage-dependent, negative regulation of EGF-stimulated cell growth.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223864 | Itga1 (untagged) - Mouse integrin alpha 1 (Itga1), (10ug) |
CNY 15,200.00 |
|
| MG222649 | Itga1 (tGFP-tagged) - Mouse integrin alpha 1 (Itga1), (10ug) |
CNY 10,550.00 |
|
| MR222649L3 | Lenti ORF clone of Itga1 (Myc-DDK-tagged) - Mouse integrin alpha 1 (Itga1) |
CNY 11,500.00 |
|
| MR222649L4 | Lenti ORF clone of Itga1 (mGFP-tagged) - Mouse integrin alpha 1 (Itga1) |
CNY 10,608.00 |
