United States
Germany
Japan
United Kingdom
China
Rock2 (NM_009072) Mouse Tagged ORF Clone
CAT＃: MR222994
- TrueORF®
Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2)
ORF Plasmid: tGFP
"NM_009072" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,496.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | B230113H15Rik; mKIAA0619; Rho-kinase; Rock-II; Rock2m; ROKalpha |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR222994 representing NM_009072
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGGCCCCCGCCGACGGGGAAAATGCCCGGCGCCCCCGAGACCGCGCCGGGGGACGGGGCGGGCG CCGGCCGTCAGAGGAAGCTGGAGGCGCTCATCCGAGACCCTCGCTCGCCCATCAACGTGGAGAGTCTGCT GGATGGCTTAAATTCCTTGGTCCTTGATTTGGATTTTCCTGCTTTGAGGAAAAATAAAAATATAGATAAC TTCTTAAATAGATATGAGAAAATTGTGAAAAAAATCAGAGGTTTACAGATGAAAGCGGAAGACTATGATG TTGTAAAAGTTATTGGAAGAGGTGCTTTTGGTGAAGTTCAGTTGGTTCGTCATAAGGCATCACAGAAGGT TTATGCAATGAAGCTTCTTAGTAAATTTGAAATGATAAAAAGATCAGATTCTGCTTTTTTCTGGGAAGAA AGAGATATTATGGCCTTTGCCAACAGTCCCTGGGTAGTTCAGCTCTTCTGTGCCTTTCAAGATGACAGGT ACCTGTACATGGTGATGGAGTACATGCCAGGCGGAGACCTCGTCAACCTTATGAGTAATTATGATGTACC TGAAAAGTGGGCCAAGTTTTACACTGCTGAAGTAGTGCTTGCACTGGATGCAATACACTCCATGGGCTTA ATACACAGAGATGTGAAGCCTGATAACATGCTATTGGATAAACATGGACATCTAAAGTTAGCAGATTTTG GCACATGTATGAAAATGGATGAAACAGGCATGGTGCATTGCGACACAGCAGTTGGAACTCCCGACTACAT ATCGCCCGAGGTTCTGAAATCACAAGGAGGTGATGGTTACTATGGGCGAGAATGTGATTGGTGGTCTGTA GGTGTTTTCCTTTTTGAGATGTTAGTAGGGGACACTCCATTTTACGCAGATTCACTTGTAGGAACCTACA GCAAAATTATGGATCATAAAAATTCACTGTGTTTTCCTGAAGATACAGAAATTTCTAAACATGCGAAGAA TCTCATATGTGCCTTCTTAACAGACAGGGAGGTACGACTTGGAAGAAATGGGGTAGAAGAAATCAAACAG CATCCTTTCTTTAAGAATGATCAATGGAATTGGGATAACATAAGAGAGACGGCAGCTCCAGTGGTACCTG AGCTCAGCAGTGACATAGACAGCAGCAACTTTGACGACATTGAGGATGACAAAGGCGATGTAGAGACCTT CCCGATTCCTAAAGCTTTTGTGGGAAATCAGCTGCCTTTTATAGGATTTACCTACTTTAGAGAAAATTTG TTATTAAGTGACTCTCCACCTTGTAGAGAAAATGATGCAATACAAACAAGGAAAAGTGAAGAAAGTCAGG AGATTCAGAAAAAATTATATGCACTAGAAGAACACCTTAGCAGTGAGGTGCAAGCCAAGGAGGAGCTGGA ACAGAAGTGCAAATCTATTAATACTCGCCTAGAGAAAACAGCAAAGGAGCTAGAAGAAGAGATTACCTTA CGGAAAAGTGTGGAATCAACATTAAGACAGCTGGAAAGAGAAAAGGCCCTTCTTCAGCACAAAAATGCAG AGTATCAGCGGAAAGCTGATCATGAAGCCGACAAGAAGCGGAACTTGGAAAATGATGTTAACAGCTTAAA AGATCAACTTGAAGATCTGAAGAAAAGGAACCAGAGCTCTCAGATATCCACTGAGAAGGTCAATCAGCTC CAGAAACAACTGGATGAAGCTAATGCTTTGCTGAGAACAGAGTCTGATACTGCAGCACGGTTAAGAAAAA CCCAGGCAGAAAGTTCCAAACAGATTCAGCAGCTGGAATCTAACAATAGAGATCTACAAGATAAAAACTG CCTGCTTGAGACTGCCAAGTTAAAACTTGAAAAGGAATTTATCAATCTTCAGTCAGCTTTAGAATCTGAA AGAAGGGACCGGACCCATGGATCAGAGATAATTAATGATTTACAAGGTAGAATATCTGGGTTGGAAGAAG ATTTGAAGACTGGCAAAGCCTTACTAGCAAAAGTAGAGCTGGAGAAGAGACAGCTGCAGGAGAAGCTCAC TGACTTAGAGAAGGAAAAAAGCAACATGGAAATAGATATGACATATCAACTGAAAGTTATACAGCAGAGT TTAGAACAAGAAGAAGCTGAACACAAGACCACAAAAGCACGGCTAGCGGATAAAAATAAGATCTATGAAT CCATTGAAGAAGCTAAATCAGAAGCCATGAAAGAAATGGAGAAGAAGCTGTTGGAAGAGAGGTCTTTAAA GCAGAAAGTGGAGAACCTGCTCCTGGAAGCCGAGAAAAGATGCTCTATATTGGACTGTGACCTCAAACAG TCTCAGCAGAAGCTAAATGAGCTGCTGAAGCAGAAAGATGTGCTGAACGAGGATGTTAGAAATTTGACAT TAAAAATAGAGCAGGAAACTCAGAAGCGTTGTCTTATGCAAAATGACCTGAAGATGCAGACCCAACAAGT TAACACCCTAAAAATGTCAGAAAAGCAAATAAAACAAGAAAATAATCATCTTATGGAAATGAAAATGAAC TTGGAAAAGCAAAATACTGAACTTCGAAAAGAGCGGCAGGATGCAGATGGGCAGATGAAGGAACTGCAAG ACCAACTTGAAGCAGAGCAGTATTTCTCAACCCTCTATAAAACACAAGTTAGAGAACTTAAAGAAGAAAA TGAAGAGAAGACAAAACTTTGTAAAGAGTTGCAACAGAAGAAGCAGGATTTACAGGATGAAAGAGACTCT TTGGCTGCCCAGCTGGAAATTACCCTTACCAAAGCAGATTCTGAGCAGCTGGCTCGTTCCATTGCTGAAG AACAATACTCTGATTTGGAAAAAGAGAAGATCATGAAAGAGCTGGAGATCAAAGAGATGATGGCTAGACA CAAACAAGAACTTACTGAAAAGGATACTACAATTGCATCTCTTGAAGAAACAAATAGGACACTAACTAGC GATGTTGCAAATCTTGCAAATGAGAAAGAAGAATTAAACAACAAGCTGAAAGATAGCCAAGAACAACTGT CCAAGTTGAAAGATGAAGAGATGAGTGCAGCAGCTATTAAAGCACAGTTTGAGAAGCAGCTTCTGAATGA GAGAACTCTCAAGACTCAAGCTGTGAATAAGTTGGCGGAGATCATGAATCGAAAAGAACCTGTCAAGCGT GGTAGTGACACAGATGTGCGAAGAAAAGAGAAGGAAAACAGAAAGCTACATATGGAGCTTAAGTCTGAAC GTGAAAAATTGACACAACAGATGATCAAATATCAGAAAGAACTGAATGAAATGCAGGCTCAAATAGCTGA AGAGAGCCAAATTCGAATAGAACTCCAGATGACCCTGGACAGTAAAGACAGCGACATTGAACAGCTGCGG TCACAGCTCCAGGCCTTGCATATTGGTATGGACAGTTCCAGTATAGGCAGTGGACCAGGCGATGCTGAGC CTGATGATGGATTTCCAGAATCAAGATTAGAAGGATGGTTGTCATTGCCTGTGCGCAACAACACTAAAAA GTTTGGATGGGTTAAAAAGTATGTGATTGTAAGCAGCAAGAAGATTCTTTTTTATGACAGCGAACAAGAT AAAGAGCAGTCCAACCCTTACATGGTGTTAGACATAGACAAGCTATTTCACGTCCGACCTGTTACCCAGA CAGATGTATACAGAGCAGATGCTAAAGAAATTCCAAGGATATTTCAGATTCTATATGCCAATGAAGGAGA AAGTAAGAAGGAACCAGAGTTTCCAGTGGAACCAGTGGGAGAAAAATCTAATTATATTTGTCACAAAGGA CATGAGTTTATTCCTACTCTTTATCACTTCCCAACCAACTGTGAGGCATGTATGAAGCCGCTGTGGCACA TGTTTAAGCCTCCTCCTGCTCTAGAGTGCCGTAGGTGCCACATTAAATGTCATAAAGATCACATGGACAA GAAGGAAGAGATTATAGCGCCCTGCAAAGTTTATTATGATATATCGTCGGCAAAGAATCTGTTGTTATTA GCAAATTCAACAGAAGAACAGCAGAAATGGGTTAGTCGGTTGGTGAAAAAGATACCTAAAAAGCCTCCAG CTCCAGACCCTTTTGCCCGATCATCCCCTAGAACTTCAATGAAAATACAACAAAACCAGTCCATTCGGCG GCCCAGTCGACAGCTTGCTCCAAACAAGCCAAGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR222994 representing NM_009072
Red=Cloning site Green=Tags(s) MSRPPPTGKMPGAPETAPGDGAGAGRQRKLEALIRDPRSPINVESLLDGLNSLVLDLDFPALRKNKNIDN FLNRYEKIVKKIRGLQMKAEDYDVVKVIGRGAFGEVQLVRHKASQKVYAMKLLSKFEMIKRSDSAFFWEE RDIMAFANSPWVVQLFCAFQDDRYLYMVMEYMPGGDLVNLMSNYDVPEKWAKFYTAEVVLALDAIHSMGL IHRDVKPDNMLLDKHGHLKLADFGTCMKMDETGMVHCDTAVGTPDYISPEVLKSQGGDGYYGRECDWWSV GVFLFEMLVGDTPFYADSLVGTYSKIMDHKNSLCFPEDTEISKHAKNLICAFLTDREVRLGRNGVEEIKQ HPFFKNDQWNWDNIRETAAPVVPELSSDIDSSNFDDIEDDKGDVETFPIPKAFVGNQLPFIGFTYFRENL LLSDSPPCRENDAIQTRKSEESQEIQKKLYALEEHLSSEVQAKEELEQKCKSINTRLEKTAKELEEEITL RKSVESTLRQLEREKALLQHKNAEYQRKADHEADKKRNLENDVNSLKDQLEDLKKRNQSSQISTEKVNQL QKQLDEANALLRTESDTAARLRKTQAESSKQIQQLESNNRDLQDKNCLLETAKLKLEKEFINLQSALESE RRDRTHGSEIINDLQGRISGLEEDLKTGKALLAKVELEKRQLQEKLTDLEKEKSNMEIDMTYQLKVIQQS LEQEEAEHKTTKARLADKNKIYESIEEAKSEAMKEMEKKLLEERSLKQKVENLLLEAEKRCSILDCDLKQ SQQKLNELLKQKDVLNEDVRNLTLKIEQETQKRCLMQNDLKMQTQQVNTLKMSEKQIKQENNHLMEMKMN LEKQNTELRKERQDADGQMKELQDQLEAEQYFSTLYKTQVRELKEENEEKTKLCKELQQKKQDLQDERDS LAAQLEITLTKADSEQLARSIAEEQYSDLEKEKIMKELEIKEMMARHKQELTEKDTTIASLEETNRTLTS DVANLANEKEELNNKLKDSQEQLSKLKDEEMSAAAIKAQFEKQLLNERTLKTQAVNKLAEIMNRKEPVKR GSDTDVRRKEKENRKLHMELKSEREKLTQQMIKYQKELNEMQAQIAEESQIRIELQMTLDSKDSDIEQLR SQLQALHIGMDSSSIGSGPGDAEPDDGFPESRLEGWLSLPVRNNTKKFGWVKKYVIVSSKKILFYDSEQD KEQSNPYMVLDIDKLFHVRPVTQTDVYRADAKEIPRIFQILYANEGESKKEPEFPVEPVGEKSNYICHKG HEFIPTLYHFPTNCEACMKPLWHMFKPPPALECRRCHIKCHKDHMDKKEEIIAPCKVYYDISSAKNLLLL ANSTEEQQKWVSRLVKKIPKKPPAPDPFARSSPRTSMKIQQNQSIRRPSRQLAPNKPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009072 |
| ORF Size | 4164 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009072.2, NP_033098.2 |
| RefSeq Size | 8012 bp |
| RefSeq ORF | 4167 bp |
| Locus ID | 19878 |
| UniProt ID | P70336 |
| MW | 161.1 kDa |
| Gene Summary | Protein kinase which is a key regulator of actin cytoskeleton and cell polarity. Involved in regulation of smooth muscle contraction, actin cytoskeleton organization, stress fiber and focal adhesion formation, neurite retraction, cell adhesion and motility via phosphorylation of ADD1, BRCA2, CNN1, EZR, DPYSL2, EP300, MSN, MYL9/MLC2, NPM1, RDX, PPP1R12A and VIM. Phosphorylates SORL1 and IRF4. Acts as a negative regulator of VEGF-induced angiogenic endothelial cell activation. Positively regulates the activation of p42/MAPK1-p44/MAPK3 and of p90RSK/RPS6KA1 during myogenic differentiation. Plays an important role in the timely initiation of centrosome duplication. Inhibits keratinocyte terminal differentiation. May regulate closure of the eyelids and ventral body wall through organization of actomyosin bundles. Plays a critical role in the regulation of spine and synaptic properties in the hippocampus. Plays a role in placental homeostasis during the perinatal period. Plays an important role in generating the circadian rhythm of the aortic myofilament Ca(2+) sensitivity and vascular contractility by modulating the myosin light chain phosphorylation.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC224381 | Rock2 (untagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug) |
CNY 17,860.00 |
|
| MG222994 | Rock2 (tGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2), (10ug) |
CNY 11,096.00 |
|
| MR222994L3 | Lenti ORF clone of Rock2 (Myc-DDK-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 14,160.00 |
|
| MR222994L4 | Lenti ORF clone of Rock2 (mGFP-tagged) - Mouse Rho-associated coiled-coil containing protein kinase 2 (Rock2) |
CNY 14,160.00 |
