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Cpeb1 (NM_007755) Mouse Tagged ORF Clone
CAT＃: MR223088
- TrueORF®
Cpeb1 (Myc-DDK-tagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1)
ORF Plasmid: tGFP
"NM_007755" in other vectors (4)
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CNY 4,192.00
CNY 4,940.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AU024112; Cpe-bp1; Cpeb; mCPEB; mCpeb-1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR223088 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCTTTCTCTCTGGAAGAAGCAGCAGGAAGAATAAAGGATTGCTGGGACAACCAAGAAGTTCCTGCAC TCTCTACATGTAGCAATGCCAATATCTTTCGAAGGATAAATGCCATTTTGGATGATTCACTGGACTTCAG TAAAGTCTGTACAACACCTATAAATCGAGGAATTCATGATCAGTTGCCAGCAGACTTCCAGGACTCTGAA GAAACAGTTACAAGCAGGATGCTTTTCCCCACCTCTGCGCAAGAATCTCCCCGTGGCCTCCCAGATGCAA ATGGCTTGTGCCTTGGCCTGCAGTCCCTCAGTCTGACTGGCTGGGACCGACCCTGGAGCACCCAGGACTC AGACTCCTCAGCCCAGAGCAGCACACAGTCAGTATTAAGCATGCTCCAAAATCCACTGGGAAATGTCTTA GGAAAAGCCCCCTTGAGCTTCCTGTCTCTGGATCCCCTTGGGTCTGACTTGGACAAGTTCCCAGCACCCT CAGTTAGAGGATCTCGCCTGGACACCCGGCCCATCCTGGACTCCCGTTCTAGCAGCCCCTCTGACTCAGA CACGAGTGGCTTCAGCTCTGGATCAGACCATCTCTCAGATTTGATTTCAAGCCTTCGCATTTCCCCTCCT CTGCCCTTTCTTTCTATGACGGGAAATGGTCCCAGAGACCCTCTAAAGATGGGCGTTGGGTCCCGAATGG ACCAAGAACAAGCTGCTCTTGCTGCAGTTGCTCCCTCTCCAACCAGTGCTCCAAAGAGATGGCCAGGAGC TTCTGTGTGGCCATCTTGGGACCTTCTTGGAGCTCCTAAAGACCCATTCAGCATAGAGAGAGAGGCTAGA CTCCACCGGCAGGCTGCAGCTGTGAATGAAGCTACGTGTACCTGGAGTGGCCAGCTTCCTCCCCGGAACT ATAAGAACCCCATCTACTCATGCAAGGTGTTCCTAGGAGGCGTTCCTTGGGATATTACAGAAGCTGGATT GGTTAACACCTTCCGTGTTTTTGGCTCTCTGAGTGTGGAGTGGCCTGGTAAGGATGGCAAGCACCCCCGG TGTCCTCCCAAAGGTAATATGCCTAAAGGGTATGTGTATTTGGTGTTTGAACTAGAGAAGTCCGTCCGGG CCTTGCTTCAGGCTTGTTCTCATGACCCGCTGAGCCCAGATGGCCTGAGTGAATATTATTTCAAGATGTC CAGCCGAAGGATGCGCTGCAAAGAGGTACAGGTAATCCCTTGGGTCTTGGCTGACAGCAACTTTGTCTGG AGCCCATCTCAGAGGCTGGATCCCAGCAGGACGGTGTTTGTTGGTGCCTTGCACGGGATGCTCAATGCTG AGGCTCTGGCTGCCATCTTGAATGACCTATTTGGTGGAGTGGTGTATGCTGGGATTGACACAGATAAGCA CAAGTACCCCATTGGGTCTGGTCGTGTGACTTTCAATAACCAACGTAGTTATCTGAAAGCAGTCACCGCT GCTTTTGTGGAGATCAAAACCACCAAGTTTACCAAGAAGGTTCAGATTGACCCCTATCTAGAAGATTCTC TGTGTCTTATCTGCAGCTCACAACCTGGTCCTTTCTTCTGCCGAGATCAGGTCTGCTTCAAGTACTTTTG CAGAAGCTGCTGGCACTGGCGACACAGCATGGAAGGCCTACGCCACCATAGCCCCCTAATGAGGAACCAG AAGAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR223088 protein sequence
Red=Cloning site Green=Tags(s) MAFSLEEAAGRIKDCWDNQEVPALSTCSNANIFRRINAILDDSLDFSKVCTTPINRGIHDQLPADFQDSE ETVTSRMLFPTSAQESPRGLPDANGLCLGLQSLSLTGWDRPWSTQDSDSSAQSSTQSVLSMLQNPLGNVL GKAPLSFLSLDPLGSDLDKFPAPSVRGSRLDTRPILDSRSSSPSDSDTSGFSSGSDHLSDLISSLRISPP LPFLSMTGNGPRDPLKMGVGSRMDQEQAALAAVAPSPTSAPKRWPGASVWPSWDLLGAPKDPFSIEREAR LHRQAAAVNEATCTWSGQLPPRNYKNPIYSCKVFLGGVPWDITEAGLVNTFRVFGSLSVEWPGKDGKHPR CPPKGNMPKGYVYLVFELEKSVRALLQACSHDPLSPDGLSEYYFKMSSRRMRCKEVQVIPWVLADSNFVW SPSQRLDPSRTVFVGALHGMLNAEALAAILNDLFGGVVYAGIDTDKHKYPIGSGRVTFNNQRSYLKAVTA AFVEIKTTKFTKKVQIDPYLEDSLCLICSSQPGPFFCRDQVCFKYFCRSCWHWRHSMEGLRHHSPLMRNQ KN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007755 |
| ORF Size | 1689 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007755.1, NM_007755.2, NM_007755.3, NM_007755.4, NM_007755.5, NP_031781.1 |
| RefSeq Size | 3138 bp |
| RefSeq ORF | 1686 bp |
| Locus ID | 12877 |
| UniProt ID | P70166 |
| MW | 62 kDa |
| Gene Summary | Sequence-specific RNA-binding protein that regulates mRNA cytoplasmic polyadenylation and translation initiation during oocyte maturation, early development and at postsynapse sites of neurons. Binds to the cytoplasmic polyadenylation element (CPE), an uridine-rich sequence element (consensus sequence 5'-UUUUUAU-3') within the 3' UTR of mRNAs. In absence of phosphorylation and in association with TACC3 is also involved as a repressor of translation of CPE-containing mRNA; a repression that is relieved by phosphorylation or degradation (By similarity). Involved in the transport of CPE-containing mRNA to dendrites; those mRNAs may be transported to dendrites in a translationally dormant form and translationally activated at synapses. Its interaction with APLP1 promotes local CPE-containing mRNA polyadenylation and translation activation. Induces the assembly of stress granules in the absence of stress (By similarity). Required for cell cycle progression, specifically for prophase entry (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
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Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC218939 | Cpeb1 (untagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10ug) |
CNY 7,130.00 |
|
| MG223088 | Cpeb1 (tGFP-tagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1), (10ug) |
CNY 5,420.00 |
|
| MR223088L3 | Lenti ORF clone of Cpeb1 (Myc-DDK-tagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1) |
CNY 6,840.00 |
|
| MR223088L4 | Lenti ORF clone of Cpeb1 (mGFP-tagged) - Mouse cytoplasmic polyadenylation element binding protein 1 (Cpeb1) |
CNY 6,840.00 |
