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Tlr8 (NM_133212) Mouse Tagged ORF Clone
CAT＃: MR224856
- TrueORF®
Tlr8 (Myc-DDK-tagged) - Mouse toll-like receptor 8 (Tlr8)
ORF Plasmid: tGFP
"NM_133212" in other vectors (4)
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CNY 10,776.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224856 representing NM_133212
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAAACATGCCCCCTCAGTCATGGATTCTGACGTGCTTTTGTCTGCTGTCCTCTGGAACCAGTGCCA TCTTCCATAAAGCGAACTATTCCAGAAGCTATCCTTGTGACGAGATAAGGCACAACTCCCTTGTGATTGC AGAATGCAACCATCGTCAACTGCATGAAGTTCCCCAAACTATAGGCAAGTATGTGACAAACATAGACTTG TCAGACAATGCCATTACACATATAACGAAAGAGTCCTTTCAAAAGCTGCAAAACCTCACTAAAATCGATC TGAACCACAATGCCAAACAACAGCACCCAAATGAAAATAAAAATGGTATGAATATTACAGAAGGGGCACT TCTCAGCCTAAGAAATCTAACAGTTTTACTGCTGGAAGACAACCAGTTATATACTATACCTGCTGGGTTG CCTGAGTCTTTGAAAGAACTTAGCCTAATTCAAAACAATATATTTCAGGTAACTAAAAACAACACTTTTG GGCTTAGGAACTTGGAAAGACTCTATTTGGGCTGGAACTGCTATTTTAAATGTAATCAAACCTTTAAGGT AGAAGATGGGGCATTTAAAAATCTTATACACTTGAAGGTACTCTCATTATCTTTCAATAACCTTTTCTAT GTGCCCCCCAAACTACCAAGTTCTCTAAGGAAACTTTTTCTGAGTAATGCCAAAATCATGAACATCACTC AGGAAGACTTCAAAGGACTGGAAAATTTAACATTACTAGATCTGAGTGGAAACTGTCCAAGGTGTTACAA TGCTCCATTTCCTTGCACACCTTGCAAGGAAAACTCATCCATCCACATACATCCTCTGGCTTTTCAAAGT CTCACCCAACTTCTCTATCTAAACCTTTCCAGCACTTCCCTCAGGACGATTCCTTCTACCTGGTTTGAAA ATCTGTCAAATCTGAAGGAACTCCATCTTGAATTCAACTATTTAGTTCAAGAAATTGCCTCGGGGGCATT TTTAACAAAACTACCCAGTTTACAAATCCTTGATTTGTCCTTCAACTTTCAATATAAGGAATATTTACAA TTTATTAATATTTCCTCAAATTTCTCTAAGCTTCGTTCTCTCAAGAAGTTGCACTTAAGAGGCTATGTGT TCCGAGAACTTAAAAAGAAGCATTTCGAGCATCTCCAGAGTCTTCCAAACTTGGCAACCATCAACTTGGG CATTAACTTTATTGAGAAAATTGATTTCAAAGCTTTCCAGAATTTTTCCAAACTCGACGTTATCTATTTA TCAGGAAATCGCATAGCATCTGTATTAGATGGTACAGATTATTCCTCTTGGCGAAATCGTCTTCGGAAAC CTCTCTCAACAGACGATGATGAGTTTGATCCACACGTGAATTTTTACCATAGCACCAAACCTTTAATAAA GCCACAGTGTACTGCTTATGGCAAGGCCTTGGATTTAAGTTTGAACAATATTTTCATTATTGGGAAAAGC CAATTTGAAGGTTTTCAGGATATCGCCTGCTTAAATCTGTCCTTCAATGCCAATACTCAAGTGTTTAATG GCACAGAATTCTCCTCCATGCCCCACATTAAATATTTGGATTTAACCAACAACAGACTAGACTTTGATGA TAACAATGCTTTCAGTGATCTTCACGATCTAGAAGTGCTGGACCTGAGCCACAATGCACACTATTTCAGT ATAGCAGGGGTAACGCACCGTCTAGGATTTATCCAGAACTTAATAAACCTCAGGGTGTTAAACCTGAGCC ACAATGGCATTTACACCCTCACAGAGGAAAGTGAGCTGAAAAGCATCTCACTGAAAGAATTGGTTTTCAG TGGAAATCGTCTTGACCGTTTGTGGAATGCAAATGATGGCAAATACTGGTCCATTTTTAAAAGTCTCCAG AATTTGATACGCCTGGACTTATCATACAATAACCTTCAACAAATCCCAAATGGAGCATTCCTCAATTTGC CTCAGAGCCTCCAAGAGTTACTTATCAGTGGTAACAAATTACGTTTCTTTAATTGGACATTACTCCAGTA TTTTCCTCACCTTCACTTGCTGGATTTATCGAGAAATGAGCTGTATTTTCTACCCAATTGCCTATCTAAG TTTGCACATTCCCTGGAGACACTGCTACTGAGCCATAATCATTTCTCTCACCTACCCTCTGGCTTCCTCT CCGAAGCCAGGAATCTGGTGCACCTGGATCTAAGTTTCAACACAATAAAGATGATCAATAAATCCTCCCT GCAAACCAAGATGAAAACGAACTTGTCTATTCTGGAGCTACATGGGAACTATTTTGACTGCACGTGTGAC ATAAGTGATTTTCGAAGCTGGCTAGATGAAAATCTGAATATCACAATTCCTAAATTGGTAAATGTTATAT GTTCCAATCCTGGGGATCAAAAATCAAAGAGTATCATGAGCCTAGATCTCACGACTTGTGTATCGGATAC CACTGCAGCTGTCCTGTTTTTCCTCACATTCCTTACCACCTCCATGGTTATGTTGGCTGCTCTGGTTCAC CACCTGTTTTACTGGGATGTTTGGTTTATCTATCACATGTGCTCTGCTAAGTTAAAAGGCTACAGGACTT CATCCACATCCCAAACTTTCTATGATGCTTATATTTCTTATGACACCAAAGATGCATCTGTTACTGACTG GGTAATCAATGAACTGCGCTACCACCTTGAAGAGAGTGAAGACAAAAGTGTCCTCCTTTGTTTAGAGGAG AGGGATTGGGATCCAGGATTACCCATCATTGATAACCTCATGCAGAGCATAAACCAGAGCAAGAAAACAA TCTTTGTTTTAACCAAGAAATATGCCAAGAGCTGGAACTTTAAAACAGCTTTCTACTTGGCCTTGCAGAG GCTAATGGATGAGAACATGGATGTGATTATTTTCATCCTCCTGGAACCAGTGTTACAGTACTCACAGTAC CTGAGGCTTCGGCAGAGGATCTGTAAGAGCTCCATCCTCCAGTGGCCCAACAATCCCAAAGCAGAAAACT TGTTTTGGCAAAGTCTGAAAAATGTGGTCTTGACTGAAAATGATTCACGGTATGACGATTTGTACATTGA TTCCATTAGGCAATAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224856 representing NM_133212
Red=Cloning site Green=Tags(s) MENMPPQSWILTCFCLLSSGTSAIFHKANYSRSYPCDEIRHNSLVIAECNHRQLHEVPQTIGKYVTNIDL SDNAITHITKESFQKLQNLTKIDLNHNAKQQHPNENKNGMNITEGALLSLRNLTVLLLEDNQLYTIPAGL PESLKELSLIQNNIFQVTKNNTFGLRNLERLYLGWNCYFKCNQTFKVEDGAFKNLIHLKVLSLSFNNLFY VPPKLPSSLRKLFLSNAKIMNITQEDFKGLENLTLLDLSGNCPRCYNAPFPCTPCKENSSIHIHPLAFQS LTQLLYLNLSSTSLRTIPSTWFENLSNLKELHLEFNYLVQEIASGAFLTKLPSLQILDLSFNFQYKEYLQ FINISSNFSKLRSLKKLHLRGYVFRELKKKHFEHLQSLPNLATINLGINFIEKIDFKAFQNFSKLDVIYL SGNRIASVLDGTDYSSWRNRLRKPLSTDDDEFDPHVNFYHSTKPLIKPQCTAYGKALDLSLNNIFIIGKS QFEGFQDIACLNLSFNANTQVFNGTEFSSMPHIKYLDLTNNRLDFDDNNAFSDLHDLEVLDLSHNAHYFS IAGVTHRLGFIQNLINLRVLNLSHNGIYTLTEESELKSISLKELVFSGNRLDRLWNANDGKYWSIFKSLQ NLIRLDLSYNNLQQIPNGAFLNLPQSLQELLISGNKLRFFNWTLLQYFPHLHLLDLSRNELYFLPNCLSK FAHSLETLLLSHNHFSHLPSGFLSEARNLVHLDLSFNTIKMINKSSLQTKMKTNLSILELHGNYFDCTCD ISDFRSWLDENLNITIPKLVNVICSNPGDQKSKSIMSLDLTTCVSDTTAAVLFFLTFLTTSMVMLAALVH HLFYWDVWFIYHMCSAKLKGYRTSSTSQTFYDAYISYDTKDASVTDWVINELRYHLEESEDKSVLLCLEE RDWDPGLPIIDNLMQSINQSKKTIFVLTKKYAKSWNFKTAFYLALQRLMDENMDVIIFILLEPVLQYSQY LRLRQRICKSSILQWPNNPKAENLFWQSLKNVVLTENDSRYDDLYIDSIRQY TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_133212 |
| ORF Size | 3096 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_133212.3, NP_573475.2 |
| RefSeq Size | 3158 bp |
| RefSeq ORF | 3099 bp |
| Locus ID | 170744 |
| UniProt ID | P58682 |
| MW | 119.8 kDa |
| Gene Summary | Key component of innate and adaptive immunity. TLRs (Toll-like receptors) control host immune response against pathogens through recognition of molecular patterns specific to microorganisms. Acts via MYD88 and TRAF6, leading to NF-kappa-B. activation, cytokine secretion and the inflammatory response (By similarity).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC223290 | Tlr8 (untagged) - Mouse toll-like receptor 8 (Tlr8), (10ug) |
CNY 13,300.00 |
|
| MG224856 | Tlr8 (tGFP-tagged) - Mouse toll-like receptor 8 (Tlr8), (10ug) |
CNY 11,310.00 |
|
| MR224856L3 | Lenti ORF clone of Tlr8 (Myc-DDK-tagged) - Mouse toll-like receptor 8 (Tlr8) |
CNY 12,160.00 |
|
| MR224856L4 | Lenti ORF clone of Tlr8 (mGFP-tagged) - Mouse toll-like receptor 8 (Tlr8) |
CNY 12,160.00 |
