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Siah2 (NM_009174) Mouse Tagged ORF Clone
CAT＃: MR224978
- TrueORF®
Siah2 (Myc-DDK-tagged) - Mouse seven in absentia 2 (Siah2)
ORF Plasmid: tGFP
"NM_009174" in other vectors (3)
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CNY 2,081.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | AA415433; Sinh2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR224978 representing NM_009174
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGCCGCCCGTCCTCCACCGGCCCCAGCGCTAACAAACCCTGCAGCAAGCAGCCGCCGCCGCCTCAGA CCCCGCACGCCCCGTCCCCGGCTGCGCCCCCGGCCGCCGCCACCATCTCGGCAGCGGGCCCCGGCTCGTC CGCGGTGCCCGCCGCGGCAGCGGTGATCTCAGGCCCCGGAGCCGGCGGCGGGGCCGACCCGGTGTCCCCG CAGCACCACGAGCTGACCTCGCTCTTCGAGTGCCCGGTCTGCTTTGACTATGTCCTGCCCCCCATTCTGC AGTGCCAGGCCGGGCACCTGGTGTGTAACCAATGCCGCCAGAAGTTAAGCTGCTGCCCGACGTGCAGGGG CGCCCTAACGCCCAGCATCAGGAACCTGGCTATGGAGAAGGTCGCCTCGGCAGTTCTGTTTCCCTGTAAG TATGCTACCACGGGCTGTTCCCTGACTCTACACCATACAGAGAAACCAGAGCATGAAGACATCTGTGAAT ACCGTCCTTATTCCTGTCCTTGTCCTGGTGCGTCCTGCAAGTGGCAGGGATCCCTGGAAGCTGTGATGTC CCATCTCATGCATGCCCACAAGAGTATCACTACCCTTCAGGGAGAGGACATAGTCTTTCTAGCTACAGAC ATTAACCTGCCAGGGGCTGTGGACTGGGTGATGATGCAGTCCTGTTTTGGCCACCACTTCATGCTGGTAC TTGAAAAGCAAGAGAAGTACGAAGGCCACCAGCAGTTCTTTGCCATCGTCCTCCTCATTGGCACCCGAAA GCAAGCTGAGAACTTTGCCTACAGACTGGAGTTGAATGGGAACCGGAGGAGACTGACCTGGGAGGCCACA CCTCGGTCCATTCATGATGGCGTGGCTGCAGCCATCATGAACAGTGACTGTCTTGTTTTTGACACAGCCA TAGCACATCTCTTTGCAGATAATGGGAACCTTGGAATCAATGTCACGATATCTACATGCTGTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR224978 representing NM_009174
Red=Cloning site Green=Tags(s) MSRPSSTGPSANKPCSKQPPPPQTPHAPSPAAPPAAATISAAGPGSSAVPAAAAVISGPGAGGGADPVSP QHHELTSLFECPVCFDYVLPPILQCQAGHLVCNQCRQKLSCCPTCRGALTPSIRNLAMEKVASAVLFPCK YATTGCSLTLHHTEKPEHEDICEYRPYSCPCPGASCKWQGSLEAVMSHLMHAHKSITTLQGEDIVFLATD INLPGAVDWVMMQSCFGHHFMLVLEKQEKYEGHQQFFAIVLLIGTRKQAENFAYRLELNGNRRRLTWEAT PRSIHDGVAAAIMNSDCLVFDTAIAHLFADNGNLGINVTISTCCQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_009174 |
| ORF Size | 975 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_009174.3, NP_033200.2 |
| RefSeq Size | 2488 bp |
| RefSeq ORF | 978 bp |
| Locus ID | 20439 |
| UniProt ID | Q06986 |
| MW | 35.2 kDa |
| Gene Summary | E3 ubiquitin-protein ligase that mediates ubiquitination and subsequent proteasomal degradation of target proteins (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). E3 ubiquitin ligases accept ubiquitin from an E2 ubiquitin-conjugating enzyme in the form of a thioester and then directly transfers the ubiquitin to targeted substrates (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). Mediates E3 ubiquitin ligase activity either through direct binding to substrates or by functioning as the essential RING domain subunit of larger E3 complexes. Mediates ubiquitination and proteasomal degradation of DYRK2 in response to hypoxia. Promotes monoubiquitination of SNCA (By similarity). Triggers the ubiquitin-mediated degradation of many substrates, including proteins involved in transcription regulation (GPS2, POU2AF1, PML, NCOR1), a cell surface receptor (DCC), an antiapoptotic protein (BAG1), and a protein involved in synaptic vesicle function in neurons (SYP) (PubMed:11257006, PubMed:14645235, PubMed:14645526, PubMed:17003045, PubMed:9637679, PubMed:26070566). It is thereby involved in apoptosis, tumor suppression, cell cycle, transcription and signaling processes. Has some overlapping function with SIAH1. Triggers the ubiquitin-mediated degradation of TRAF2, whereas SIAH1 does not. Regulates cellular clock function via ubiquitination of the circadian transcriptional repressors NR1D1 and NR1D2 leading to their proteasomal degradation. Plays an important role in mediating the rhythmic degradation/clearance of NR1D1 and NR1D2 contributing to their circadian profile of protein abundance (PubMed:26392558).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MG224978 | Siah2 (tGFP-tagged) - Mouse seven in absentia 2 (Siah2), (10ug) |
CNY 2,850.00 |
|
| MR224978L3 | Lenti ORF clone of Siah2 (Myc-DDK-tagged) - Mouse seven in absentia 2 (Siah2) |
CNY 4,750.00 |
|
| MR224978L4 | Lenti ORF clone of Siah2 (mGFP-tagged) - Mouse seven in absentia 2 (Siah2) |
CNY 4,750.00 |
