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Lclat1 (NM_001081071) Mouse Tagged ORF Clone
CAT＃: MR226111
- TrueORF®
Lclat1 (Myc-DDK-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 1
ORF Plasmid: tGFP
"NM_001081071" in other vectors (4)
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CNY 5,856.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | Agpat8; AI181996; Alcat1; Gm91; Lycat |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226111 representing NM_001081071
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGTCATGGAAGGGGATTTACTTTATACTCTTCCTGTTTGCTGGAAGCTTTTTTGGAAGTATTTTTA TGCTCGGCCCCATTTTACCTTTGATGTTTATAAACCTGTCGTGGTATCGCTGGATTAGCAGCCGTCTTGT GGCTACATGGCTCACACTTCCTGTGGCATTGCTGGAGACCATGTTTGGTGTGAGAGTGGTTATAACAGGT GACGCCTTTGTGCCTGGAGAGCGGAGCGTCATCATCATGAACCACCGGACACGTGTGGACTGGATGTTCC TGTGGAACTGTCTAATGAGGTACAGCTACCTCAGGGTGGAGAAGATTTGCCTCAAATCCAGTCTCAAAAG TGTTCCTGGATTCGGCTGGGCCATGCAAGTTGCGGCCTTTATCTTTATTCATAGGAAGTGGAAGGATGAT AAGAGCCATTTTGAAGACATGATTGATTATTTTTGTGCCATCCATGAACCACTACAGCTTCTCATTTTTC CAGAAGGAACTGACCTCACAGAAAATAATAAGGCTAGGAGTAATGATTTTGCTGAGAAGAACGGACTTCA GAAATATGAGTATGTTTTACACCCAAGAACCACTGGCTTTACCTTTGTGGTGGACCGCCTAAGAGAAGGG AAGAACCTCGATGCTGTTCATGACATCACGGTCGCATATCCTTACAACATCCCTCAAACTGAGAAGCACC TTCTCCTTGGAGACTTTCCCAAGGAGATCCACTTCCACGTCCAGCGGTATCCAGCTGACTCTCTTCCCAC ATCCAAGGAGGACCTTCAGCTCTGGTGCCACAGAAGGTGGGAAGAAAAGGAGGAGAGGCTTCGGTCCTTC TACCAAGGAGAGAAAAACTTCCACTTTACTGGGCAGAGTACAGTTCCACCTTGCAAGTCTGAGCTCAGAG TCCTTGTGGTCAAGCTACTGTCCATAGTGTACTGGGCCTTGTTCTGCTCTGCAATGTGCCTGCTCATATA TCTGTACAGCCCTGTTCGGTGGTATTTTATAATCAGCATTGTGTTCTTCGTGCTGCAGGAGAGAATATTT GGTGGACTGGAAATCATTGAACTTGCATGTTACCGTTTTTTACACAAGCACCCACATTTAAATTCAAAGA AAAATGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226111 representing NM_001081071
Red=Cloning site Green=Tags(s) MVSWKGIYFILFLFAGSFFGSIFMLGPILPLMFINLSWYRWISSRLVATWLTLPVALLETMFGVRVVITG DAFVPGERSVIIMNHRTRVDWMFLWNCLMRYSYLRVEKICLKSSLKSVPGFGWAMQVAAFIFIHRKWKDD KSHFEDMIDYFCAIHEPLQLLIFPEGTDLTENNKARSNDFAEKNGLQKYEYVLHPRTTGFTFVVDRLREG KNLDAVHDITVAYPYNIPQTEKHLLLGDFPKEIHFHVQRYPADSLPTSKEDLQLWCHRRWEEKEERLRSF YQGEKNFHFTGQSTVPPCKSELRVLVVKLLSIVYWALFCSAMCLLIYLYSPVRWYFIISIVFFVLQERIF GGLEIIELACYRFLHKHPHLNSKKNE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001081071 |
| ORF Size | 1128 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001081071.2, NP_001074540.1 |
| RefSeq Size | 4476 bp |
| RefSeq ORF | 1131 bp |
| Locus ID | 225010 |
| UniProt ID | Q3UN02 |
| MW | 44.8 kDa |
| Gene Summary | Exhibits acyl-CoA:lysocardiolipin acyltransferase (ALCAT) activity; catalyzes the reacylation of lyso-cardiolipin to cardiolipin (CL), a key step in CL remodeling (PubMed:15152008). Recognizes both monolysocardiolipin and dilysocardiolipin as substrates with a preference for linoleoyl-CoA and oleoyl-CoA as acyl donors (PubMed:15152008). Also exhibits 1-acyl-sn-glycerol-3-phosphate acyltransferase activity (AGPAT) activity; converts 1-acyl-sn-glycerol-3- phosphate (lysophosphatidic acid or LPA) into 1,2-diacyl-sn-glycerol-3- phosphate (phosphatidic acid or PA) by incorporating an acyl moiety at the sn-2 position of the glycerol backbone (By similarity). Possesses lysophosphatidylinositol acyltransferase (LPIAT) activity (PubMed:20668164). Possesses lysophosphatidylglycerol acyltransferase (LPGAT) activity (By similarity). Required for establishment of the hematopoietic and endothelial lineages (PubMed:17675553).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC212743 | Lclat1 (untagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 1, (10ug) |
CNY 3,990.00 |
|
| MG226111 | Lclat1 (tGFP-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1) transcript variant 1, (10ug) |
CNY 3,140.00 |
|
| MR226111L3 | Lenti ORF clone of Lclat1 (Myc-DDK-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 1 |
CNY 4,750.00 |
|
| MR226111L4 | Lenti ORF clone of Lclat1 (mGFP-tagged) - Mouse lysocardiolipin acyltransferase 1 (Lclat1), transcript variant 1 |
CNY 4,750.00 |
