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Htr2c (NM_008312) Mouse Tagged ORF Clone
CAT＃: MR226671
- TrueORF®
Htr2c (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c)
ORF Plasmid: tGFP
"NM_008312" in other vectors (4)
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CNY 5,488.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Mouse Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | 5-HT2C; 5-HT2cR; 5-HTR2C; 5HT1c; Htr1; Htr1c; S; SR1 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>MR226671 representing NM_008312
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGTGAACCTGGGCACTGCGGTGCGCTCACTCCTTGTGCACCTAATTGGCCTATTGGTTTGGCAGTTCG ATATTTCCATAAGTCCAGTAGCAGCTATAGTAACTGACACTTTTAATTCCTCCGATGGTGGACGCTTGTT TCAATTCCCGGACGGGGTACAAAACTGGCCAGCACTTTCAATAGTCGTGATTATAATCATGACAATAGGG GGCAACATTCTCGTTATCATGGCAGTAAGCATGGAGAAGAAACTGCACAATGCTACCAATTATTTCTTAA TGTCCCTAGCCATTGCTGATATGCTGGTGGGACTACTTGTCATGCCCCTGTCTCTGCTTGCAATTCTTTA TGATTATGTCTGGCCTTTACCTAGATATTTGTGCCCCGTCTGGATTTCACTAGATGTGCTATTTTCAACT GCGTCCATCATGCACCTCTGCGCCATATCGCTGGACCGGTATGTAGCAATACGTAATCCTATTGAGCATA GCCGGTTCAATTCGCGGACTAAGGCCATCATGAAGATTGCCATCGTTTGGGCAATATCAATAGGAGTTTC AGTTCCTATCCCTGTGATTGGACTGAGGGACGAAAGCAAAGTGTTCGTGAATAATACTACCTGCGTGCTC AATGACCCGAACTTCGTTCTCATCGGGTCCTTCGTGGCATTCTTCATCCCGTTGACAATTATGGTGATCA CCTACTTCTTAACGATCTACGTCCTACGCCGTCAAACCCTGATGTTACTTCGAGGTCACACCGAGGAGGA ACTGCGTAATATCAGCCTGAACTTTCTAAAGTGCTGCTGCAAGAAGGGTGATGAGGAAGAGAACGCTCCC AACCCCAATCCAGATCAGAAGCCACGTCGAAAGAAGAAAGAAAAGCGGCCTAGAGGCACCATGCAAGCTA TCAACAATGAGAAGAAAGCTTCCAAAGTCCTTGGCATTGTATTCTTTGTGTTTCTGATCATGTGGTGCCC GTTTTTCATCACCAATATCCTGTCGGTGCTTTGTGGGAAGGCCTGTAACCAAAAGCTAATGGAGAAACTT CTCAATGTGTTTGTTTGGATTGGCTATGTGTGTTCAGGCATCAATCCTCTGGTGTACACTCTCTTCAACA AAATTTACCGAAGGGCTTTCTCTAAATATTTGCGCTGCGATTATAAGCCAGACAAAAAGCCTCCTGTTCG ACAGATTCCTAGGGTTGCTGCCACTGCTTTGTCTGGGAGGGAGCTCAATGTTAACATTTATCGGCATACC AATGAACGTGTAGTTAGGAAAGCTAATGACACAGAGCCTGGCATAGAGATGCAGGTAGAGAATTTAGAGC TGCCGGTCAATCCCTCTAATGTGGTCAGCGAGAGGATTAGTAGTGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >MR226671 representing NM_008312
Red=Cloning site Green=Tags(s) MVNLGTAVRSLLVHLIGLLVWQFDISISPVAAIVTDTFNSSDGGRLFQFPDGVQNWPALSIVVIIIMTIG GNILVIMAVSMEKKLHNATNYFLMSLAIADMLVGLLVMPLSLLAILYDYVWPLPRYLCPVWISLDVLFST ASIMHLCAISLDRYVAIRNPIEHSRFNSRTKAIMKIAIVWAISIGVSVPIPVIGLRDESKVFVNNTTCVL NDPNFVLIGSFVAFFIPLTIMVITYFLTIYVLRRQTLMLLRGHTEEELRNISLNFLKCCCKKGDEEENAP NPNPDQKPRRKKKEKRPRGTMQAINNEKKASKVLGIVFFVFLIMWCPFFITNILSVLCGKACNQKLMEKL LNVFVWIGYVCSGINPLVYTLFNKIYRRAFSKYLRCDYKPDKKPPVRQIPRVAATALSGRELNVNIYRHT NERVVRKANDTEPGIEMQVENLELPVNPSNVVSERISSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_008312 |
| ORF Size | 1377 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_008312.4, NP_032338.3 |
| RefSeq Size | 4765 bp |
| RefSeq ORF | 1380 bp |
| Locus ID | 15560 |
| UniProt ID | P34968 |
| MW | 52.4 kDa |
| Gene Summary | Serotonin (5-hydroxytryptamine, 5-HT), a neurotransmitter, elicits a wide array of physiological effects by binding to several receptor subtypes, including the 5-HT2 family of seven-transmembrane-spanning, G-protein-coupled receptors, which activate phospholipase C and D signaling pathways. This gene encodes the 2C subtype of serotonin receptor and its mRNA is subject to multiple RNA editing events, where genomically encoded adenosine residues are converted to inosines. RNA editing is predicted to alter amino acids within the second intracellular loop of the 5-HT2C receptor and generate receptor isoforms that differ in their ability to interact with G proteins and the activation of phospholipase C and D signaling cascades, thus modulating serotonergic neurotransmission in the central nervous system. Studies in rodents show altered patterns of RNA editing in response to drug treatments and stressful situations. [provided by RefSeq, Jul 2008] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
Evaluation of Lorcaserin as an Anticonvulsant in Juvenile Fmr1 Knockout Mice
,Saraf, T;Felsing, D;Armstrong, J;Booth, R;Canal, C;,
Epilepsy Research
,PubMed ID 34130255
[HTR2C]
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Documents
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| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| MC216115 | Htr2c (untagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c), (10ug) |
CNY 5,488.00 |
|
| MG226671 | Htr2c (tGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c), (10ug) |
CNY 7,088.00 |
|
| MR226671L3 | Lenti ORF clone of Htr2c (Myc-DDK-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c) |
CNY 6,460.00 |
|
| MR226671L4 | Lenti ORF clone of Htr2c (mGFP-tagged) - Mouse 5-hydroxytryptamine (serotonin) receptor 2C (Htr2c) |
CNY 6,460.00 |
