Grk6 (NM_001286063) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Gprk6
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230504 representing NM_001286063 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGCAGTTCCCCCATATCAGCCAGTGTGAGGAGCTTCGACTCAGCCTTGAGCGTGACTACCACAGCC TATGTGAGCGCCAGCCCATTGGGCGCCTGTTATTTCGTGAGTTCTGTGCTACGAGACCTGAGCTGACCCG GTGTACTGCCTTCCTGGATGGGGTGTCTGAATATGAGGTGACCCCTGATGAGAAGCGGAAAGCATGTGGG CGCCGACTAATGCAGAACTTTCTGAGCCACACGGGTCCTGACCTCATCCCTGAAGTTCCACGGCAGCTGG TGAGTAACTGTGCCCAGCGGCTAGAGCAGGGACCCTGCAAAGACCTCTTCCAGGAGCTGACCCGGCTGAC CCACGAGTACCTGAGCACGGCCCCTTTTGCCGACTACCTCGACAGCATCTACTTCAACCGTTTTCTGCAG TGGAAGTGGCTGGAAAGGCAACCAGTGACCAAAAACACCTTCAGGCAGTACCGAGTCCTGGGCAAAGGTG GCTTTGGGGAGGTATGTGCCTGCCAGGTGCGGGCAACAGGCAAGATGTACGCATGCAAGAAACTGGAAAA GAAGCGGATAAAGAAGCGAAAGGGGGAGGCCATGGCTCTCAACGAGAAACAGATCTTGGAGAAAGTGAAC AGTAGGTTTGTAGTGAGCTTAGCCTACGCCTATGAGACCAAGGATGCACTGTGCCTGGTGCTGACATTGA TGAATGGAGGTGACCTAAAGTTCCACATCTACCACATGGGCCAGGCTGGCTTTCCTGAAGCACGTGCTGT CTTCTATGCTGCTGAGATCTGCTGTGGCCTGGAAGACCTGCACCGGGAACGCATTGTGTACAGGGATCTA AAGCCAGAGAATATCCTTCTGGATGACCATGGCCACATTCGGATCTCGGACCTGGGACTGGCCGTGCATG TGCCTGAGGGCCAGACCATCAAAGGCCGTGTGGGCACTGTGGGCTACATGGCTCCAGAGGTGGTGAGGAA TGAGCGCTACACGTTCAGTCCTGACTGGTGGGCGCTAGGCTGCCTCCTGTACGAGATGATCGCAGGTCAG TCGCCCTTCCAGCAGAGGAAGAAGAAGATAAAGCGCGAAGAGGTGGAGCGGCTGGTCAAGGAAGTGGCCG AGGAGTACACAGACCGCTTTTCCTCACAGGCGCGCTCACTCTGTTCTCAGCTTCTTAGCAAGGACCCTGC TGAGCGCCTGGGGTGTCGTGGAGGTGGCGCCCGTGAGGTAAAGGAGCACCCCCTTTTCAAGAAACTGAAT TTCAAGCGGCTGGGAGCTGGCATGCTAGAGCCACCTTTTAAGCCTGATCCCCAGGCTATTTATTGCAAGG ATGTCCTGGACATTGAACAGTTCTCTACAGTTAAAGGTGTGGATCTGGAGCCCACAGACCAAGACTTCTA CCAGAAGTTTGCCACAGGCAGTGTGTCCATCCCCTGGCAGAATGAGATGGTGGAGACCGAGTGCTTCCAG GAACTCAATGTCTTTGGGCTGGATGGGTCTGTTCCCCCAGACCTGGACTGGAAGGGCCAGCCCACTGCAC CCCCCAAGAAGGGATTGCTACAGAGACTCTTCAGTCGCCAAAGGATTGCTGTGGGAACTGCAGCGACAGT GAGGAAGAGCTCCCCACCCGCCTCTAGCCCCCAGGCCGAGGCCCCCACCGGCGGTTGGCGG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230504 representing NM_001286063 Red=Cloning site Green=Tags(s) MLQFPHISQCEELRLSLERDYHSLCERQPIGRLLFREFCATRPELTRCTAFLDGVSEYEVTPDEKRKACG RRLMQNFLSHTGPDLIPEVPRQLVSNCAQRLEQGPCKDLFQELTRLTHEYLSTAPFADYLDSIYFNRFLQ WKWLERQPVTKNTFRQYRVLGKGGFGEVCACQVRATGKMYACKKLEKKRIKKRKGEAMALNEKQILEKVN SRFVVSLAYAYETKDALCLVLTLMNGGDLKFHIYHMGQAGFPEARAVFYAAEICCGLEDLHRERIVYRDL KPENILLDDHGHIRISDLGLAVHVPEGQTIKGRVGTVGYMAPEVVRNERYTFSPDWWALGCLLYEMIAGQ SPFQQRKKKIKREEVERLVKEVAEEYTDRFSSQARSLCSQLLSKDPAERLGCRGGGAREVKEHPLFKKLN FKRLGAGMLEPPFKPDPQAIYCKDVLDIEQFSTVKGVDLEPTDQDFYQKFATGSVSIPWQNEMVETECFQ ELNVFGLDGSVPPDLDWKGQPTAPPKKGLLQRLFSRQRIAVGTAATVRKSSPPASSPQAEAPTGGWR TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001286063
ORF Size	1671 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001286063.1, NP_001272992.1
RefSeq Size	2996 bp
RefSeq ORF	1674 bp
Locus ID	26385
UniProt ID	O70293
MW	64 kDa
Gene Summary	Specifically phosphorylates the activated forms of G protein-coupled receptors. Such receptor phosphorylation initiates beta-arrestin-mediated receptor desensitization, internalization, and signaling events leading to their desensitization. Seems to be involved in the desensitization of D2-like dopamine receptors in striatum and chemokine receptor CXCR4 which is critical for CXCL12-induced cell chemotaxis (By similarity). Phosphorylates rhodopsin (RHO) (in vitro) and a non G-protein-coupled receptor, LRP6 during Wnt signaling (in vitro) (By similarity).[UniProtKB/Swiss-Prot Function]

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