Ralbp1 (NM_001198949) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	Rip1; RLIP76
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR230786 representing NM_001198949 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACTGAGTGCTTCCTGCCCCCGAGCAGCAGCCCTAGTGAACACCGCAGGGCCGAGCATGGCAGCGGGC TGACCCGGACCCCCAGCTCTGAGGAGATCAGTCCTACAAAGTTCCCTGGATTGTACCGGACAGGCGAGCC CTCGCCTCCCCATGACGTCCTCCATGAGCCTCCTGATACAGTGTCAGATGACGACAAAGACCATGGAAAG AAAAAAGGGAAATTTAAGAAAAAGGAGAAAAGGACTGAAGGCTACGCTGCCTTCCAGGAAGACAGCTCTG GAGATGAAGCTGAAAGTCCTTCCAAGGTGAAGAGGTCCAAGGGAATCCATGTGTTCAAAAAGCCCAGTTT CTCTAAAAAGAAGGAAAAAGATTTTAAAATCAAAGAAAAACCCAAAGAAGAAAAGCATAAAGAAGAAAAG CACAAAGAGGAAAAGCATAAAGAAAAGAAATCGAAAGACTTGACAGCAGCTGATGTTGTTAAACAGTGGA AGGAAAAGAAGAAAAAGAAAAAGCCAATTCAGGAGCCAGAGGTGCCTCAGATGGACGCACCGAGTGTCAA GCCCATTTTTGGGGTTCCCTTGGTTGACGCAGTAGAGAGGACCATGATGTACGACGGCGTCCGGCTGCCA GCTGTCTTCCGGGAGTGTGTGGACTACATGGAGAAGCACGGCATGAAGTGTGAAGGCGTCTACCGGGTGT CAGGAATCAAATCAAAGGTAGATGAGCTAAAAGCAGCCTATGACCGAGAGGAGTCTCCAAACTTAGAAGA ATATGAGCCTAACACTGTAGCCAGCTTGCTGAAGCAGTATTTGCGAGACCTTCCAGAGAATTTGCTTACC AAAGAGCTTATGCCCCGGTTTGAAGAGGCTTGTGGGAAGACCACAGAGATGGAGAAAGTGCAGGAATTCC AGCGCTTGCTCCGGGAACTGCCGGAGTGCAATCATCTTCTGCTTTCCTGGCTCATTGTGCACCTGGACCA CGTCATTGCGAAGGAGCTGGAAACGAAGATGAACATCCAGAACATCTCTATAGTGCTGAGCCCCACCGTG CAGATCAGCAATCGGGTCCTGTACGTGCTTTTCACACATGTGCAAGAGCTCTTTGGCACCGTGGTCCTGA AGCAAGTAACAAGACCTCTGCGCTGGTCCAACATGGCCACGATGCCCACACTGCCAGAGACCCAAGCAGG CATCAAGGAGGAGATCAGGAGACAGGAGTTCCTTTTGAATTGTTTACATCGAGATCTGCAGGGCGGGATA AAGGACTTATCTAAAGAAGAAAGATTATGGGAAGTACAGAGGATTCTGACTGCCCTCAAGAGAAAGCTGA GGGAAGCTAAAAGACAAGAGTGTGAAACCAAGATTGCACAGGAGATAGCTAGTCTTTCAAAAGAGGATGT TTCCAAAGAAGAAATGAATGAAAACGAAGAAGTTATAAATATCCTTCTTGCCCAGGAGAATGAAATCCTG ACAGAACAGGAGGAGCTCCTGGCCATGGAGCAGTTCCTGCGTCGTCAGATCGCCTCAGAGAAAGAAGAGA TCGACCGCCTCCGAGCTGAAATTGCGGAGATCCAGAGCCGTCAGCAGCATGGCCGCAGTGAGACGGAGGA GTACTCGTCCGACAGCGAGAGTGAGAGCGAGGACGAGGAGGAGCTGCAGCTCATCCTGGAGGACCTGCAG CGGCAGAACGAGGAGTTAGAAATAAAGAACAATCACTTGAACCAAGCAGTCCATGAGGAGCGGGAGGCCA TCATCGAGCTCCGAGTGCAGCTCCGGCTGCTCCAGATGCAGCGAGCCAAGTCTGAGCAGCAGCCGCAGGA GGAGGAGGAGCCCGAGAGGCGAGGGGGCATAGGCCCACCGCCCTGTGACGGTGTCCTCGAGGTGCGGGTA GCCAAGGAGCAGGCGAAGGCCTCGCCCAGCAAAGACCGGAAGGAGACGCCCATC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR230786 representing NM_001198949 Red=Cloning site Green=Tags(s) MTECFLPPSSSPSEHRRAEHGSGLTRTPSSEEISPTKFPGLYRTGEPSPPHDVLHEPPDTVSDDDKDHGK KKGKFKKKEKRTEGYAAFQEDSSGDEAESPSKVKRSKGIHVFKKPSFSKKKEKDFKIKEKPKEEKHKEEK HKEEKHKEKKSKDLTAADVVKQWKEKKKKKKPIQEPEVPQMDAPSVKPIFGVPLVDAVERTMMYDGVRLP AVFRECVDYMEKHGMKCEGVYRVSGIKSKVDELKAAYDREESPNLEEYEPNTVASLLKQYLRDLPENLLT KELMPRFEEACGKTTEMEKVQEFQRLLRELPECNHLLLSWLIVHLDHVIAKELETKMNIQNISIVLSPTV QISNRVLYVLFTHVQELFGTVVLKQVTRPLRWSNMATMPTLPETQAGIKEEIRRQEFLLNCLHRDLQGGI KDLSKEERLWEVQRILTALKRKLREAKRQECETKIAQEIASLSKEDVSKEEMNENEEVINILLAQENEIL TEQEELLAMEQFLRRQIASEKEEIDRLRAEIAEIQSRQQHGRSETEEYSSDSESESEDEEELQLILEDLQ RQNEELEIKNNHLNQAVHEEREAIIELRVQLRLLQMQRAKSEQQPQEEEEPERRGGIGPPPCDGVLEVRV AKEQAKASPSKDRKETPI TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001198949
ORF Size	1944 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001198949.1, NP_001185878.1
RefSeq Size	3788 bp
RefSeq ORF	1947 bp
Locus ID	19765
UniProt ID	Q62172
MW	75.5 kDa
Gene Summary	Can activate specifically hydrolysis of GTP bound to RAC1 and CDC42, but not RALA. Mediates ATP-dependent transport of S-(2,4-dinitrophenyl)-glutathione (DNP-SG) and doxorubicin (DOX) and is the major ATP-dependent transporter of glutathione conjugates of electrophiles (GS-E) and DOX in erythrocytes. Can catalyze transport of glutathione conjugates and xenobiotics, and may contribute to the multidrug resistance phenomenon. Serves as a scaffold protein that brings together proteins forming an endocytotic complex during interphase and also with CDK1 to switch off endocytosis, One of its substrates would be EPN1/Epsin (By similarity).[UniProtKB/Swiss-Prot Function]

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