Pde10a (NM_001290707) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231255 representing NM_001290707 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCGAAGAAGCGCAAAGCCCTCGAGGGTGGAGGAGAACCCCAGCTCCCAGAGGAGGAACCCACTGCCT GGTTTGGGGGCAGCAGCGAGGAGCCAGGCTGCCTCCCATTCGCACTTAAAGGTGGGAGCAAGGGCCCCGC TCTATTGGCACTTCGAAACCGCACAGACTCTCGGGGGCAGATGAGCAATGACTCCACAGAAGGCACCGTG GGCTCCTGCAACGCAACAGGTTTGACGGATGAAAAGGTGAAGGCCTATCTTTCTCTCCATCCCCAGGTAT TAGATGAATTTGTTTCTGAAAGTGTTAGTGCAGAGACTGTGGAAAAGTGGCTGAAGAGGAAAACCAACAA AGCAAAAGATGAACCATCTCCCAAGGAAGTCAGCAGGTACCAGGATACGAATATGCAGGGAGTCGTGTAC GAGCTGAACAGCTACATAGAGCAGCGCCTGGACACGGGCGGGGACAACCACCTGCTCCTCTATGAGCTCA GCAGCATCATCAGGATAGCCACAAAAGCCGACGGATTTGCACTGTACTTCCTTGGAGAGTGCAATAATAG CCTGTGTGTGTTCATACCACCCGGGATGAAGGAAGGCCAACCCCGGCTCATCCCTGCAGGGCCCATCACC CAGGGTACCACCATCTCTGCCTACGTGGCCAAGTCTAGGAAGACGTTGTTGGTAGAGGATATCCTTGGGG ATGAGCGATTTCCTCGAGGTACTGGCCTGGAATCAGGAACCCGCATCCAGTCTGTTCTTTGCTTGCCCAT TGTCACTGCCATTGGAGACTTGATTGGCATCCTTGAACTGTACAGGCACTGGGGCAAAGAGGCCTTCTGC CTCAGCCATCAGGAGGTTGCAACAGCCAATCTTGCTTGGGCTTCCGTAGCAATACACCAGGTGCAGGTGT GTAGAGGTCTCGCCAAACAGACCGAACTGAATGACTTCCTACTCGACGTATCAAAGACATACTTTGATAA CATAGTTGCCATAGACTCTCTACTTGAACACATCATGATATATGCAAAAAATCTAGTGAACGCCGACCGC TGCGCGCTCTTCCAGGTGGACCACAAGAACAAGGAGCTGTACTCGGACCTGTTTGACATTGGGGAGGAGA AGGAGGGGAAGCCCATCTTCAAGAAGACCAAGGAGATCAGATTTTCCATTGAGAAAGGGATTGCTGGTCA AGTGGCAAGAACAGGCGAAGTCTTGAACATTCCCGATGCCTACGCGGACCCTCGCTTTAACAGGGAGGTG GACCTGTACACAGGCTACACCACGAGGAACATTCTGTGTATGCCCATAGTGAGCCGAGGCAGCGTGATTG GCGTGGTGCAGATGGTGAACAAGATCAGCGGTAGCGCCTTCTCCAAGACAGACGAGAACAACTTCAAGAT GTTTGCTGTCTTCTGCGCACTGGCCTTGCACTGTGCTAACATGTACCACAGGATCCGCCACTCAGAATGC ATCTACAGGGTTACCATGGAGAAGCTTTCCTACCACAGCATCTGCACCTCCGAGGAGTGGCAAGGCCTCA TGCGCTTCAACCTACCAGCACGCATCTGCCGGGACATCGAGCTATTCCACTTTGACATTGGTCCTTTCGA GAACATGTGGCCTGGGATCTTTGTCTACATGATCCATCGGTCTTGTGGGACATCCTGTTTTGAACTTGAA AAATTGTGCCGTTTTATCATGTCTGTGAAGAAGAACTATCGGCGGGTTCCTTACCACAACTGGAAGCATG CAGTCACGGTGGCACACTGCATGTATGCCATACTTCAAAACAACAATGGCCTCTTCACAGACCTCGAGCG CAAAGGCCTGCTAATTGCGTGTCTGTGCCATGACCTGGACCACAGGGGCTTCAGTAACAGCTACCTGCAG AAGTTCGACCACCCCCTGGCGGCGCTGTACTCCACCTCCACCATGGAGCAACACCACTTCTCCCAGACGG TGTCCATCCTTCAGCTGGAAGGGCACAATATCTTCTCCACCCTGAGCTCCAGCGAGTACGAGCAGGTGCT GGAGATCATCCGCAAAGCCATCATCGCCACCGACCTCGCCCTATACTTTGGGAACAGGAAGCAGTTGGAG GAGATGTACCAGACAGGGTCGCTGAACCTCCACAACCAGTCCCATCGAGACCGTGTCATCGGCTTGATGA TGACTGCCTGTGATCTTTGCTCTGTGACCAAACTATGGCCAGTTACAAAATTGACAGCGAATGATATATA TGCAGAATTCTGGGCTGAGGGTGATGAGATGAAGAAGCTGGGCATACAGCCCATTCCTATGATGGACAGA GACAAGCGAGATGAAGTCCCTCAAGGGCAGCTCGGATTCTACAATGCTGTGGCCATTCCCTGCTATACCA CCTTGACGCAGATCCTCCCACCCACAGAGCCTCTGCTGAAGGCCTGCAGGGATAACCTCAATCAGTGGGA GAAGGTAATTCGCGGGGAAGAGACAGCAATGTGGATTTCAGGCCCAGGCCCGGCGCCTAGCAAGAGCACA CCTGAGAAGCTGAACGTGAAGGTTGAAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231255 representing NM_001290707 Red=Cloning site Green=Tags(s) MSKKRKALEGGGEPQLPEEEPTAWFGGSSEEPGCLPFALKGGSKGPALLALRNRTDSRGQMSNDSTEGTV GSCNATGLTDEKVKAYLSLHPQVLDEFVSESVSAETVEKWLKRKTNKAKDEPSPKEVSRYQDTNMQGVVY ELNSYIEQRLDTGGDNHLLLYELSSIIRIATKADGFALYFLGECNNSLCVFIPPGMKEGQPRLIPAGPIT QGTTISAYVAKSRKTLLVEDILGDERFPRGTGLESGTRIQSVLCLPIVTAIGDLIGILELYRHWGKEAFC LSHQEVATANLAWASVAIHQVQVCRGLAKQTELNDFLLDVSKTYFDNIVAIDSLLEHIMIYAKNLVNADR CALFQVDHKNKELYSDLFDIGEEKEGKPIFKKTKEIRFSIEKGIAGQVARTGEVLNIPDAYADPRFNREV DLYTGYTTRNILCMPIVSRGSVIGVVQMVNKISGSAFSKTDENNFKMFAVFCALALHCANMYHRIRHSEC IYRVTMEKLSYHSICTSEEWQGLMRFNLPARICRDIELFHFDIGPFENMWPGIFVYMIHRSCGTSCFELE KLCRFIMSVKKNYRRVPYHNWKHAVTVAHCMYAILQNNNGLFTDLERKGLLIACLCHDLDHRGFSNSYLQ KFDHPLAALYSTSTMEQHHFSQTVSILQLEGHNIFSTLSSSEYEQVLEIIRKAIIATDLALYFGNRKQLE EMYQTGSLNLHNQSHRDRVIGLMMTACDLCSVTKLWPVTKLTANDIYAEFWAEGDEMKKLGIQPIPMMDR DKRDEVPQGQLGFYNAVAIPCYTTLTQILPPTEPLLKACRDNLNQWEKVIRGEETAMWISGPGPAPSKST PEKLNVKVED TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290707
ORF Size	2550 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290707.1, NP_001277636.1
RefSeq Size	7763 bp
RefSeq ORF	2553 bp
Locus ID	23984
UniProt ID	Q8CA95
MW	96.2 kDa
Gene Summary	Plays a role in signal transduction by regulating the intracellular concentration of cyclic nucleotides. Can hydrolyze both cAMP and cGMP, but has higher affinity for cAMP and is more efficient with cAMP as substrate. May play a critical role in regulating cAMP and cGMP levels in the striatum, a region of the brain that contributes to the control of movement and cognition.[UniProtKB/Swiss-Prot Function]

*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.