Plk4 (NM_173169) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	1700028H20; AI385771; S; Sak; Stk1; Stk18
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231346 representing NM_173169 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCGGCGTGCATCGGGGAGAGGATCGAGGACTTTAAGGTTGGAAATCTACTCGGTAAAGGATCATTTG CTGGTGTCTACAGAGCTGAGTCCATACACACTGGTTTGGAAGTTGCAATCAAAATGATAGATAAGAAAGC CATGTACAAAGCTGGAATGGTACAGAGAGTCCAAAATGAGGTGAAAATACATTGCCAGTTGAAACACCCC TCTGTCTTGGAGCTCTATAATTACTTTGAAGATAACAATTATGTCTACCTGGTATTGGAAATGTGCCACA ATGGAGAAATGAACAGATATCTGAAGAACAGAATGAAGCCTTTCTCAGAAAGGGAAGCTAGGCACTTCAT GCACCAGATTATCACAGGAATGTTATATCTTCATTCTCATGGCATATTGCACCGGGACCTCACACTCTCT AACATCTTACTTACGCGGAATATGAACATAAAAATTGCTGACTTTGGACTAGCAACGCAGTTGAATATGC CACATGAAAAGCACTATACACTCTGTGGGACTCCTAATTATATTTCACCAGAAATTGCAACTCGAAGTGC ACATGGACTTGAATCTGATATTTGGTCATTGGGCTGTATGTTTTATACGTTACTTATTGGAAGACCACCT TTTGACACTGACACAGTCAAGAACACATTGAACAAAGTAGTCCTGGCAGATTATGAAATGCCAGCCTTTT TGTCACGAGAGGCCCAGGACCTTATCCACCAGTTACTTCGTAGAAACCCTGCAGATCGGTTAAGTCTGTC TTCTGTGTTGGACCATCCTTTCATGTCACGAAATCCTTCACCAAAGAGTAAAGACGTAGGGACTGTAGAG GACTCAATGGATAGTGGGCATGCTACACTTTCCACAACAATTACAGCCTCTTCTGGTACCAGTTTGAGTG GCAGCCTACTTGACAGAAGACTTTTGGTTGGTCAACCACTTCCAAATAAAATTACTGTATTTCAAAAAAA TAAAAATTCAAGTGACTTTTCTTCAGGAGATGGAAGTAATTTTTGTACTCAATGGGGAAATCCAGAACAA GAAGCTAATAGTAGGGGACGGGGGAGAGTGATTGAAGATGCAGAAGAGAGGCCGCATTCTCGATACCTGC GCAGAGCTCATTCCTCTGATAGAGCCAGCCCCTCTAATCAGTCTCGAGCAAAAACATACTCAGTAGAACG TTGTCACTCAGTAGAAATGCTTTCAAAGCCTAGAAGATCACTGGATGAAAATCAACACAGTTCCAATCAT CATTGTCTAGGAAAAACTCCTTTTCCATTTGCAGACCAGACACCTCAGATGGAAATGGTACAGCAGTGGT TTGGGAATCTGCAAATGAATGCTCATTTAGGAGAAACTAATGAGCACCACACCGTTAGCCCAAACAGAGA TTTCCAGGACTATCCAGATTTGCAGGACACGTTACGAAACGCTTGGACTGACACGAGAGCCAGCAAGAAT GCTGATACTTCTGCCAATGTTCATGCTGTAAAGCAGCTGAGTGCCATGAAATACATGAGTGCACATCACC ATAAGCCTGAGGTCATGCCACAGGAGCCGGGCCTACATCCTCATTCTGAACAAAGCAAGAATAGAAGTAT GGAGTCGACACTGGGTTACCAGAAACCTACCTTAAGAAGTATTACATCTCCTCTGATTGCTCACAGATTA AAGCCAATCAGACAGAAAACCAAAAAGGCTGTGGTGAGCATCCTTGATTCAGAGGAGATCACTGTTTATT ACCCGAACGATGGAAGAGGCTTTCCTCTTGCTGACAGACCTCCCTTGCCTACTGACAACATCAGTAGGTA CAGCTTTGACAATCTACCAGAAAAATACTGGCGGAAATATCAGTATGCTTCCAGATTCATTCAGCTAGTA AGATCTAAAACTCCCAAAATCACTTATTTTACAAGATATGCTAAATGTATTTTGATGGAAAATTCTCCTG GTGCTGATTTCGAAGTTTGGTTTTATGATGGAGCCAAAATACATAAAACTGAAAATTTAATTCACATAAT TGAGAAAACAGGGATATCTTATAATTTAAAAAATGAAAATGAAGTTACCAGCCTGAAAGAGGAAGTAAAA GTATATATGGACCATGCTAATGAGGGTCACCGTATTTGCTTGTCACTGGAATCTGTAATCTCTGAGGAGG AAAAGAGAAGCAGGGGTTCTTCATTCTTCCCTATAATCGTAGGAAGAAAACCTGGTAATACTAGTTCACC TAAAGCCTTATCAGCTCCTCCTGTGGACCCAAGCTGCTGTAAGGGAGAGCAGGCGTCAGCAAGCAGACTG AGCGTGAATAGTGCCGCTTTCCCCACACAGTCCCCAGGACTCAGTCCTTCCACTGTGACAGTTGAAGGAC TTGGCCACACAGCGACTGCCACAGGAACAGGCGTCTCTTCAAGTCTTCCTAAATCTGCACAGCTTTTGAA ATCTGTTTTTGTGAAAAATGTTGGTTGGGCTACACAGCTAACTAGCGGAGCTGTGTGGGTTCAGTTTAAT GATGGGTCACAGTTGGTTGTCCAGGCAGGAGTATCTTCCATCAGTTACACATCACCAGATGGTCAGACAA CTAGGTATGGAGAAAATGAAAAATTACCTGAATACATCAAACAGAAATTACAGTGTCTTTCTTCCATCCT TCTGATGTTTTCTAATCCAACTCCTAATTTTCAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231346 representing NM_173169 Red=Cloning site Green=Tags(s) MAACIGERIEDFKVGNLLGKGSFAGVYRAESIHTGLEVAIKMIDKKAMYKAGMVQRVQNEVKIHCQLKHP SVLELYNYFEDNNYVYLVLEMCHNGEMNRYLKNRMKPFSEREARHFMHQIITGMLYLHSHGILHRDLTLS NILLTRNMNIKIADFGLATQLNMPHEKHYTLCGTPNYISPEIATRSAHGLESDIWSLGCMFYTLLIGRPP FDTDTVKNTLNKVVLADYEMPAFLSREAQDLIHQLLRRNPADRLSLSSVLDHPFMSRNPSPKSKDVGTVE DSMDSGHATLSTTITASSGTSLSGSLLDRRLLVGQPLPNKITVFQKNKNSSDFSSGDGSNFCTQWGNPEQ EANSRGRGRVIEDAEERPHSRYLRRAHSSDRASPSNQSRAKTYSVERCHSVEMLSKPRRSLDENQHSSNH HCLGKTPFPFADQTPQMEMVQQWFGNLQMNAHLGETNEHHTVSPNRDFQDYPDLQDTLRNAWTDTRASKN ADTSANVHAVKQLSAMKYMSAHHHKPEVMPQEPGLHPHSEQSKNRSMESTLGYQKPTLRSITSPLIAHRL KPIRQKTKKAVVSILDSEEITVYYPNDGRGFPLADRPPLPTDNISRYSFDNLPEKYWRKYQYASRFIQLV RSKTPKITYFTRYAKCILMENSPGADFEVWFYDGAKIHKTENLIHIIEKTGISYNLKNENEVTSLKEEVK VYMDHANEGHRICLSLESVISEEEKRSRGSSFFPIIVGRKPGNTSSPKALSAPPVDPSCCKGEQASASRL SVNSAAFPTQSPGLSPSTVTVEGLGHTATATGTGVSSSLPKSAQLLKSVFVKNVGWATQLTSGAVWVQFN DGSQLVVQAGVSSISYTSPDGQTTRYGENEKLPEYIKQKLQCLSSILLMFSNPTPNFQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_173169
ORF Size	2694 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_173169.2, NP_775261.2
RefSeq Size	3486 bp
RefSeq ORF	2697 bp
Locus ID	20873
UniProt ID	Q64702
MW	101.3 kDa
Gene Summary	This gene encodes a member of the polo family of serine/threonine protein kinases. The protein localizes to the nucleolus during G2, to centrosomes during G2/M, and to the cleavage furrow during cytokinesis. It is required for progression through mitosis, cell survival, and embryonic development. The mouse genome contains a pseudogene similar to this gene. Three transcript variants encoding two different isoforms have been found for this gene. [provided by RefSeq, Nov 2011]

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