Prrt3 (NM_001289700) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6330505P20; B230206N24Rik
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231448 representing NM_001289700 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCCCAGCCCACAGGCCTGCACATCTCCTCTTCTGCTGTTGCTGCTGCCATGCCTGGGGGCTGGCC CTGCCTTGGGCAGAGGCCTTCCCAGGCCCCTGGAGAACTCAGAACCTCACATGATCCCATCAGAGTCACA GACCTTTGACCTCTTCTGGGAGAAGCTGAGAAATGAGAGTTCCTGGCACAGCGGTGACCCTCAGGCCCGT GCTGAGGGGCCCAAGAAGCCCGCAGACCCTTACCTAGGACCAGCCCTGCATGGGCCCAAGGCAGCCCCTG GGGTCCAAGGTGAAAGACTACTAAGAGCTGATGACCTCCAGCTGGCCCGAGCATTCACCTCCCAAGGCTG GACAGGACCTCCTGACTCACAGGAGCTTCTGGAGCCTGAGGCACCAGAACCCCACCCTGTGCGAGCACCT CGTCTCACTCTTGTCACTACAACTCCCAGCTCCCTACTCTCGGCAGCCATACTTTCCACTGCCTCCCAGA AGCCTGGAGGCACAGCAGGGCAGCAGCCAGCTAGGAACGAGGAGCTAATAATGGTCAAAGCCGAGACCCA CATCACACAGGCTTCCCCCTGGGACTTCCAGGGCTCTTCCCACACTCCGGTACCCGAGACAGATGCTGTG AGGACGCTGGTGTTGGGGAAGCAGGGGGGACATGAACAGGGCTTCCAGGAGGCTGTCCAGGGTCCCCTAC TCACCCAGCAGGATCCAGTAGTCCCTGGGGTCGGCTCAACACCCCCAGTTAAAGTGGAATCCACCCCAGA GCCTGGAGCCCAGCTCGACCTGGCATTGGTCAGAAGCCTTCCTCTTCCTGAGGGGCTGCCAGCTGAACCC CCCAAAACTGGAGCTGGGGATACCTGGGAAGTCAGCTCTCTGGGACCCCAACCAGAACAGACCGATCTCC TTGGAGGACAAGATTCCCCAGCACCCCAGCCTATACCACCTTCAGCCTCAGACACTTCTGATGGGCACCT AAGACCAGCAGTATCCTCGCTGAATGGAGCCGACCCCATCTCCCCACAACGTGTGAGAGGAGCGATGGAG GCCCCAGGAACCCCCAAGTCCTTCATCCCTGACCTTCCAAATTCTGCTCAAGCTGCAAATGGAACAGAGA GCCCTGTGAGGGCCCTGCAGCCAGATGAAGCCGAGGATTGGCCGGGTCGCCCACAAAGCCACCCTCCAGC ACCCCCTGTCCAGGCCCCCTCGACATCTCGGCGTGGTCTCGTTAGAGTCACCACACAGAGAGCTCTGGGC CAGCCACTCCCTCCGGAGCCCTCAGCCAGCTCCATAGTCCCCATCCCAGCTTCTAGCCCCCCAGCCAATG CCACCGCACCTCCCCTGCGTTGGGGACCCCTTCGGCGGGTGCTGAGTTTCTCCTGGGAGCTTCATGTGTA TGGGGTGGGGGTGCTTTTCCTGCTTCCAGCATTATTGGCATTGGTCACAGTGGCAGCCGCCCTAGCGGGA CCCCGATTAGCACTAGTGGCTGCCGCGCTGGTATTGGTGGCTTCAGGGCTGCGATCTGCCTACATGCTCA CGGATCCTTATGGCTCTCAGGCGCGACTGGGAGTACGTGCAGGTCTGGTGCTATACAACTTGCCCTTCCC CTTGCTACTCACTGCACTGGCAGCACTCACCCTGCTGGGCTTGGGTGCTGGTCTGCCACAGCCTCTGCAG AAGCCGCTGCTGCTGGGAATTGTGGCACCGGTGCATGGCACATGTTTGCTGGCTACAGACCTGTTCTCCA CCAGCCCTGTGCTCAACCTTCTAACACAAGGCTTATCTTGCGCCTGGGGCGCCTCAGTGGCTCTGGGCAC ACTCTGCCTGTGCCGTCGACGCCTGCTGGAAGGCCCTCGGGGCTGGGATGCCAGTCCAGGGCCCCGGCTG TTGGCTGTGGCGGGCTCACTGGGGCTGTTGGCCAGTGGTCTGCAGTTGGCGGCCTCTCTCTGGCTGTACC CAGGGCCTGGCCGGGAGGGTCGCTTCTCCTGGGCTTGGTGGGGCGTGCACTTCTGGCTGCGCCTGCTGGA ATTGACCTGGGCACTGGCCCTGGCGCTAGCTGCCCTGGCTGCCACTCGTCCCAGGCCGCCCACGGAGCAC GCTTGCTGGGCTAAGCTGCTGCGTCTGGCGTGCCCCGCGCCTACAGGGAAGAGCGAAGTTCCCGAGAGAC CCAATAACTGCTATGCGGGGCCCAGTGGCCTGGGCACAGGCGGCCTGGACATCAGCAAAAGTCTCATCCG CAACGCTGCTGGGGAAGCTGGGCTTCCTGTCACTCCTGGTTCTGGACCCTGGGGTTCGGCTGCATCGCTG GGTCGCGGTCGTCCAGGTGGCCAGAGGATGTCCCGGGGCAGTGTGGGGCCCGCACCGTCGCTGAGCGAGC TGGATCTGCGGCCACCGTCACCTATCAATTTGAGCCGCAGCATCGACGCCGCGCTCTTCCGGGAGCACTT GGTTCGAGAGAGTGTGTTCCAACGCTGTGGTCTCCGTGGCTTGGCCTCCTCGCCGACAGGGGGCGCCCTG CGGCCTCGCCGCGGCAGCCAGCCCGATGCCGAGCTCGACGGCGCGGGCACATCATTGCTCCGAGGCCGCT GCCGCTCGCTCACCGAGGTGTGCTTGCGCACGTCGCTCCCGCAGCACGTGATGGAGCCGCCCGTCGGCGC GGCGGCCGCGGGGACTTCGGGCAGCTCCCTGGACAGTTTCTCCAAGGGCTCGCTGAAGATCAGCTGGAAC CCGTGGCGTCACGGGCTGTCATCCGTGGACAGCCTGCCCCTAGATGAACTGCCCAGCACCGTGCAGCTGC TGCCACCACCTACCCCAGTCCCTGCTCCTGCCCGGGCGGGGGAGCCTCAGGGCGAGGGCCAGTCGCGTTG CAAGTCCAGCGAGTCTCACAGCGCCTCTAGTGACACTATAGAGCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231448 representing NM_001289700 Red=Cloning site Green=Tags(s) MAPSPQACTSPLLLLLLPCLGAGPALGRGLPRPLENSEPHMIPSESQTFDLFWEKLRNESSWHSGDPQAR AEGPKKPADPYLGPALHGPKAAPGVQGERLLRADDLQLARAFTSQGWTGPPDSQELLEPEAPEPHPVRAP RLTLVTTTPSSLLSAAILSTASQKPGGTAGQQPARNEELIMVKAETHITQASPWDFQGSSHTPVPETDAV RTLVLGKQGGHEQGFQEAVQGPLLTQQDPVVPGVGSTPPVKVESTPEPGAQLDLALVRSLPLPEGLPAEP PKTGAGDTWEVSSLGPQPEQTDLLGGQDSPAPQPIPPSASDTSDGHLRPAVSSLNGADPISPQRVRGAME APGTPKSFIPDLPNSAQAANGTESPVRALQPDEAEDWPGRPQSHPPAPPVQAPSTSRRGLVRVTTQRALG QPLPPEPSASSIVPIPASSPPANATAPPLRWGPLRRVLSFSWELHVYGVGVLFLLPALLALVTVAAALAG PRLALVAAALVLVASGLRSAYMLTDPYGSQARLGVRAGLVLYNLPFPLLLTALAALTLLGLGAGLPQPLQ KPLLLGIVAPVHGTCLLATDLFSTSPVLNLLTQGLSCAWGASVALGTLCLCRRRLLEGPRGWDASPGPRL LAVAGSLGLLASGLQLAASLWLYPGPGREGRFSWAWWGVHFWLRLLELTWALALALAALAATRPRPPTEH ACWAKLLRLACPAPTGKSEVPERPNNCYAGPSGLGTGGLDISKSLIRNAAGEAGLPVTPGSGPWGSAASL GRGRPGGQRMSRGSVGPAPSLSELDLRPPSPINLSRSIDAALFREHLVRESVFQRCGLRGLASSPTGGAL RPRRGSQPDAELDGAGTSLLRGRCRSLTEVCLRTSLPQHVMEPPVGAAAAGTSGSSLDSFSKGSLKISWN PWRHGLSSVDSLPLDELPSTVQLLPPPTPVPAPARAGEPQGEGQSRCKSSESHSASSDTIEL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001289700
ORF Size	2916 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001289700.1, NP_001276629.1
RefSeq Size	3710 bp
RefSeq ORF	2919 bp
Locus ID	210673
UniProt ID	Q6PE13
MW	101.7 kDa

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