Slc4a10 (NM_001242380) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	mKIAA4136; NCBE
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231594 representing NM_001242380 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAGATTAAAGACCAGGGAGCCCAAATGGAGCCGCTGCTGCCTACGAGAAATGATGAAGAAGCCGTTG TGGATAGAGGTGGAACACGCTCTATTCTCAAAACACATTTTGAGAAAGAAGATTTAGAAGGTCATCGGAC ATTATTTATTGGAGTTCATGTGCCCCTGGGTGGAAGAAAAAGCCATCGTCGTCACAGGCATCGTGGTCAT AAGCACAGAAAGAGGGACAGAGAGAGAGATTCGGGACTGGAGGATGGAAGAGAGTCCCCTTCTTTTGACA CCCCATCGCAGAGGGTGCAGTTTATTCTTGGAACTGAGGACGATGATGAGGAGCACCTCCCTCATGACCT TTTCACAGAGCTGGATGAGATTTGCTGGCGTGAAGGGGAAGATGCTGAGTGGCGAGAGACAGCCAGGTGG TTGAAATTTGAAGAGGATGTGGAAGATGGAGGAGAAAGATGGAGTAAGCCCTATGTGGCCACGCTTTCAT TACACAGCTTGTTTGAGTTGAGAAGCTGCATCCTGAATGGAACTGTGCTACTGGACATGCATGCCAACAC GATAGAAGAAATTGCAGATATGGTCCTTGACCAGCAGGTCAGCTCAGGCCAGCTGAATGAAGATGTTCGC CACAGGGTCCACGAAGCATTGATGAAGCAGCATCATCACCAGAATCAGAAAAAACTGGCTAACAGGATTC CTATTGTCCGATCTTTTGCTGATATTGGCAAGAAACAATCAGAACCAAATTCCATGGATAAAAATGCAGG TCAGGTTGTTTCTCCTCAGTCTGCTCCAGCCTGTGCTGAGAATAAAAATGATGTCAGCAGGGAAAACAGC ACTGTAGACTTCAGCAAGGGACTGGGAGGCCAACAAAAGGGGCATACTAGTCCATGTGGGATGAAACAAA GGCTTGACAAAGGACCTCCACACCAGCAGGAGAGAGAGGTTGATCTGCATTTTATGAAAAAGATTCCTCC GGGTGCTGAAGCTTCAAACATCTTGGTAGGAGAACTGGAGTTCCTAGACAGAACTGTGGTTGCCTTTGTC AGGTTGTCTCCAGCTGTCTTGCTCCAAGGACTTGCTGAAGTTCCAATCCCAAGCAGATTTCTGTTCATCC TTCTGGGACCCCTGGGAAAGGGTCAACAGTACCACGAGATTGGCAGATCGATTGCGACCTTAATGACTGA TGAGGTGTTTCATGATGTTGCTTACAAAGCTAAAGACCGCAATGACTTGGTATCAGGAATTGATGAGTTT CTGGATCAGGTTACCGTTCTTCCTCCTGGAGAATGGGATCCAAGCATACGAATAGAACCTCCCAAAAATG TCCCTTCCCAGGAGAAGAGGAAGATTCCTGCTGTACCAAATGGAACAGCAGCTCATGGCGAAGCTGAGCC ACATGGAGGACACAGCGGACCTGAACTCCAGCGAACTGGGAGGATTTTTGGGGGACTTATATTAGATATC AAAAGAAAGGCTCCATTCTTCTGGAGTGACTTCAGGGATGCTTTCAGCCTGCAGTGCTTAGCATCGTTCC TGTTTCTCTACTGTGCATGCATGTCTCCTGTCATCACATTTGGAGGACTGTTGGGAGAAGCAACTGAAGG TCGTATAAGTGCAATCGAATCACTCTTTGGAGCATCTATGACCGGGATAGCCTATTCTCTTTTTGGTGGA CAGCCCCTGACCATATTAGGCAGCACAGGACCTGTTTTGGTGTTTGAAAAGATCTTGTTTAAGTTTTGCA AGGAATACGGCCTGTCGTACTTGTCCTTACGGGCCAGCATTGGGCTCTGGACTGCAACACTGTGCATCAT CCTTGTGGCCACGGACGCGAGCTCACTCGTCTGCTACATCACCCGGTTTACCGAAGAGGCTTTTGCTTCT CTCATTTGCATCATTTTTATCTATGAAGCCCTGGAGAAGTTGTTTGAGCTCAGTGAAACCTATCCAATCA ATATGCACAATGATTTGGAACTGCTGACACAATACTCATGTAACTGTATGGAGCCACATAGTCCCAGCAA TGACACACTGAAGGAATGGCGGGAGTCCAACCTTTCTGCCTCTGACATAATCTGGGGGAACCTAACTGTG TCAGAGTGCAGATCACTGCACGGGGAGTATGTCGGGCGAGCCTGTGGCCATGGCCACCCCTACGTGCCAG ATGTTCTCTTCTGGTCGGTGATCCTGTTCTTCTCCACAGTTACCATGTCAGCCACCCTGAAGCAGTTCAA GACCAGCCGCTATTTCCCAACCAAGGTTCGATCCATAGTGAGTGATTTTGCGGTTTTTCTTACAATTCTG TGTATGGTTTTAATTGACTATGCCATTGGGATCCCATCACCAAAACTACAAGTACCAAGCGTTTTCAAGC CGACCAGAGACGACCGTGGCTGGTTTGTTACACCTTTGGGTCCAAACCCATGGTGGACAATCATAGCTGC CATCATCCCAGCTTTACTCTGTACTATTCTGATTTTCATGGACCAGCAGATTACAGCTGTCATCATCAAC AGAAAAGAGCACAAGCTAAAGAAAGGTTGTGGCTATCACCTGGATCTGTTAATGGTGGCAGTCATGCTCG GGGTCTGCTCCATTATGGGCCTGCCATGGTTTGTGGCTGCCACAGTTCTCTCCATCACTCATGTCAACAG CCTCAAGCTCGAATCAGAGTGCTCTGCTCCAGGAGAACAACCCAAGTTTCTCGGCATTCGGGAGCAGAGG GTGACCGGGCTCATGATTTTTATTCTTATGGGTTCATCCGTTTTCATGACCAGCATTCTGAAGTTTATCC CCATGCCAGTGTTATACGGAGTGTTTCTTTATATGGGTGCTTCGTCTCTCAAAGGAATTCAGTTATTTGA TAGAATAAAGCTCTTCTGGATGCCAGCCAAACATCAACCAGATTTCATCTATCTAAGGCACGTGCCCCTC CGGAAAGTCCATCTCTTCACAGTCATTCAGATGAGTTGTCTCGGCCTTCTGTGGATAATCAAAGTTTCGA GAGCTGCTATTGTCTTTCCTATGATGGTGTTGGCACTAGTGTTTGTGAGAAAGTTGATGGACTTCTTGTT TACCAAACGGGAACTCAGCTGGCTTGATGATTTAATGCCTGAGAGTAAAAAGAAGAAACTTGAAGATGCT GAGAAAGAAGAAGAACAAAGTATGCTAGCCATGGAGGACGAGGGCACAGTACAACTCCCACTGGAGGGAC ACTACAGAGACGACCCGTCTGTGATCAATATTTCTGATGAAATGTCAAAGACTGCCATGTGGGGGAACCT TCTAGTTACTGCTGACAACTCAAAAGAAAAGGAGTCACGCTTTCCTTCTAAAAGCTCCCCTTCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231594 representing NM_001242380 Red=Cloning site Green=Tags(s) MEIKDQGAQMEPLLPTRNDEEAVVDRGGTRSILKTHFEKEDLEGHRTLFIGVHVPLGGRKSHRRHRHRGH KHRKRDRERDSGLEDGRESPSFDTPSQRVQFILGTEDDDEEHLPHDLFTELDEICWREGEDAEWRETARW LKFEEDVEDGGERWSKPYVATLSLHSLFELRSCILNGTVLLDMHANTIEEIADMVLDQQVSSGQLNEDVR HRVHEALMKQHHHQNQKKLANRIPIVRSFADIGKKQSEPNSMDKNAGQVVSPQSAPACAENKNDVSRENS TVDFSKGLGGQQKGHTSPCGMKQRLDKGPPHQQEREVDLHFMKKIPPGAEASNILVGELEFLDRTVVAFV RLSPAVLLQGLAEVPIPSRFLFILLGPLGKGQQYHEIGRSIATLMTDEVFHDVAYKAKDRNDLVSGIDEF LDQVTVLPPGEWDPSIRIEPPKNVPSQEKRKIPAVPNGTAAHGEAEPHGGHSGPELQRTGRIFGGLILDI KRKAPFFWSDFRDAFSLQCLASFLFLYCACMSPVITFGGLLGEATEGRISAIESLFGASMTGIAYSLFGG QPLTILGSTGPVLVFEKILFKFCKEYGLSYLSLRASIGLWTATLCIILVATDASSLVCYITRFTEEAFAS LICIIFIYEALEKLFELSETYPINMHNDLELLTQYSCNCMEPHSPSNDTLKEWRESNLSASDIIWGNLTV SECRSLHGEYVGRACGHGHPYVPDVLFWSVILFFSTVTMSATLKQFKTSRYFPTKVRSIVSDFAVFLTIL CMVLIDYAIGIPSPKLQVPSVFKPTRDDRGWFVTPLGPNPWWTIIAAIIPALLCTILIFMDQQITAVIIN RKEHKLKKGCGYHLDLLMVAVMLGVCSIMGLPWFVAATVLSITHVNSLKLESECSAPGEQPKFLGIREQR VTGLMIFILMGSSVFMTSILKFIPMPVLYGVFLYMGASSLKGIQLFDRIKLFWMPAKHQPDFIYLRHVPL RKVHLFTVIQMSCLGLLWIIKVSRAAIVFPMMVLALVFVRKLMDFLFTKRELSWLDDLMPESKKKKLEDA EKEEEQSMLAMEDEGTVQLPLEGHYRDDPSVINISDEMSKTAMWGNLLVTADNSKEKESRFPSKSSPS TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001242380
ORF Size	3354 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001242380.1, NP_001229309.1
RefSeq Size	5553 bp
RefSeq ORF	3357 bp
Locus ID	94229
UniProt ID	Q5DTL9
MW	126.3 kDa
Gene Summary	Sodium/bicarbonate cotransporter which plays an important role in regulating intracellular pH (PubMed:10993873, PubMed:20566632). Has been shown to act as a sodium/bicarbonate cotransporter in exchange for intracellular chloride (PubMed:10993873, PubMed:20566632). Has also been shown to act as a sodium/biocarbonate cotransporter which is not responsible for net efflux of chloride, with the observed chloride efflux being due to chloride self-exchange (By similarity). Controls neuronal pH and may contribute to the secretion of cerebrospinal fluid (PubMed:18165320). Reduces the excitability of CA1 pyramidal neurons and modulates short-term synaptic plasticity (PubMed:26136660). Required in retinal cells to maintain normal pH which is necessary for normal vision (PubMed:23056253). In the kidney, likely to mediate bicarbonate reclamation in the apical membrane of the proximal tubules (By similarity).[UniProtKB/Swiss-Prot Function]

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