Tek (NM_001290549) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AA517024; Cd202b; Hyk; STK1; Tie-2; Tie2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231600 representing NM_001290549 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGACTCTTTAGCCGGCTTAGTTCTCTGTGGAGTCAGCTTGCTCCTTTATGGAGTAGTAGAAGGCGCCA TGGACCTGATCTTGATCAATTCCCTACCTCTTGTGTCTGATGCCGAAACATCCCTCACCTGCATTGCCTC TGGGTGGCACCCCCATGAGCCCATCACCATAGGAAGGGACTTTGAAGCCTTAATGAACCAGCACCAAGAT CCACTGGAGGTTACTCAAGATGTGACCAGAGAATGGGCGAAAAAAGTTGTTTGGAAGAGAGAAAAGGCCA GTAAGATTAATGGTGCTTATTTCTGTGAAGGTCGAGTTCGAGGACAGGCTATAAGGATACGGACCATGAA GATGCGTCAACAAGCATCCTTCCTACCTGCTACTTTAACTATGACCGTGGACAGGGGAGATAATGTGAAC ATATCTTTCAAAAAGGTGTTAATTAAAGAAGAAGATGCAGTGATTTACAAAAATGGCTCCTTCATCCACT CAGTGCCCCGGCATGAAGTACCTGATATTTTAGAAGTTCACTTGCCGCATGCTCAGCCCCAGGATGCTGG TGTGTACTCGGCCAGGTACATAGGAGGAAACCTGTTCACCTCAGCCTTCACCAGGCTGATTGTTCGGAGA TGTGAAGCTCAGAAGTGGGGGCCCGACTGTAGCCGTCCTTGTACTACTTGCAAGAACAATGGAGTCTGCC ATGAAGATACCGGGGAATGCATTTGCCCTCCTGGGTTTATGGGGAGAACATGTGAGAAAGCTTGTGAGCC GCACACATTTGGCAGGACCTGTAAAGAAAGGTGTAGTGGACCAGAAGGATGCAAGTCTTATGTGTTCTGT CTCCCAGACCCTTACGGGTGTTCCTGTGCCACAGGCTGGAGGGGGTTGCAGTGCAATGAAGCATGCCCAT CTGGTTACTACGGACCAGACTGTAAGCTCAGGTGCCACTGTACCAATGAAGAGATATGTGATCGGTTCCA AGGATGCCTCTGCTCTCAAGGATGGCAAGGGCTGCAGTGTGAGAAAGAAGGCAGGCCAAGGATGACTCCA CAGATAGAGGATTTGCCAGATCACATTGAAGTAAACAGTGGAAAATTTAACCCCATCTGCAAAGCCTCTG GGTGGCCACTACCTACTAGTGAAGAAATGACCCTAGTGAAGCCAGATGGGACAGTGCTCCAACCAAATGA CTTCAACTATACAGATCGTTTCTCAGTGGCCATATTCACTGTCAACCGAGTCTTACCTCCTGACTCAGGA GTCTGGGTCTGCAGTGTGAACACAGTGGCTGGGATGGTGGAAAAGCCTTTCAACATTTCCGTCAAAGTTC TTCCAGAGCCCCTGCACGCCCCAAATGTGATTGACACTGGACATAACTTTGCTATCATCAATATCAGCTC TGAGCCTTACTTTGGGGATGGACCCATCAAATCCAAGAAGCTTTTCTATAAACCTGTCAATCAGGCCTGG AAATACATTGAAGTGACGAATGAGATTTTCACTCTCAACTACTTGGAGCCGCGGACTGACTACGAGCTGT GTGTGCAGCTGGCCCGTCCTGGAGAGGGTGGAGAAGGGCATCCTGGGCCTGTGAGACGATTTACAACAGC GTCTATCGGACTCCCTCCTCCAAGAGGTCTCAGTCTCCTGCCAAAAAGCCAGACAGCTCTAAATTTGACT TGGCAACCGATATTTACAAACTCAGAAGATGAATTTTATGTGGAAGTCGAGAGGCGATCCCTGCAAACAA CAAGTGATCAGCAGAACATCAAAGTGCCTGGGAACCTGACCTCGGTGCTACTGAGCAACTTAGTCCCCAG GGAGCAGTACACAGTCCGAGCTAGAGTCAACACCAAGGCGCAGGGGGAGTGGAGTGAAGAACTCAGGGCC TGGACCCTTAGTGACATTCTCCCTCCTCAACCAGAAAACATCAAGATCTCCAACATCACTGACTCCACAG CTATGGTTTCTTGGACAATAGTGGATGGCTATTCGATTTCTTCCATCATCATCCGGTATAAGGTTCAGGG CAAAAATGAAGACCAGCACATTGATGTGAAGATCAAGAATGCTACCGTTACTCAGTACCAGCTCAAGGGC CTAGAGCCAGAGACTACATACCATGTGGATATTTTTGCTGAGAACAACATAGGATCAAGCAACCCAGCCT TTTCTCATGAACTGAGGACGCTTCCACATTCCCCAGCCTCTGCAGACCTCGGAGGGGGAAAGATGCTACT CATAGCCATCCTTGGGTCGGCTGGAATGACTTGCATCACCGTGCTGTTGGCGTTTCTGATTATGTTGCAA CTGAAGAGAGCAAATGTCCAAAGGAGAATGGCTCAGGCATTCCAGAACAGAGAAGAACCAGCTGTGCAGT TTAACTCAGGAACTCTGGCCCTTAACAGGAAGGCCAAAAACAATCCGGATCCCACAATTTATCCTGTGCT TGACTGGAATGACATCAAGTTTCAAGACGTGATCGGAGAGGGCAACTTTGGCCAGGTTCTGAAGGCACGC ATCAAGAAGGATGGGTTACGGATGGATGCCGCCATCAAGAGGATGAAAGAGTATGCCTCCAAAGATGATC ACAGGGACTTCGCAGGAGAACTGGAGGTTCTTTGTAAACTTGGACACCATCCAAACATCATTAATCTCTT GGGAGCATGTGAACACCGAGGCTATTTGTACCTAGCTATTGAGTATGCCCCGCATGGAAACCTCCTGGAC TTCCTGCGTAAGAGCAGAGTGCTAGAGACAGACCCTGCTTTTGCCATCGCCAACAGTACAGCTTCCACAC TGTCCTCCCAACAGCTTCTTCATTTTGCTGCAGATGTGGCCCGGGGGATGGACTACTTGAGCCAGAAACA GTTTATCCACAGGGACCTGGCTGCCAGAAACATTTTAGTTGGTGAAAACTACATAGCCAAAATAGCAGAT TTTGGATTGTCACGAGGTCAAGAAGTGTATGTGAAAAAGACAATGGGAAGGCTCCCAGTGCGTTGGATGG CAATCGAATCACTGAACTATAGTGTCTATACAACCAACAGTGATGTCTGGTCCTATGGTGTATTGCTCTG GGAGATTGTTAGCTTAGGAGGCACCCCCTACTGCGGCATGACGTGCGCGGAGCTCTATGAGAAGCTACCC CAGGGCTACAGGCTGGAGAAGCCCCTGAACTGTGATGATGAGGTGTATGATCTAATGAGACAGTGCTGGA GGGAGAAGCCTTATGAGAGACCATCATTTGCCCAGATATTGGTGTCCTTAAACAGGATGCTGGAAGAACG GAAGACATACGTGAACACCACACTGTATGAGAAGTTTACCTATGCAGGAATTGACTGCTCTGCGGAAGAA GCAGCC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231600 representing NM_001290549 Red=Cloning site Green=Tags(s) MDSLAGLVLCGVSLLLYGVVEGAMDLILINSLPLVSDAETSLTCIASGWHPHEPITIGRDFEALMNQHQD PLEVTQDVTREWAKKVVWKREKASKINGAYFCEGRVRGQAIRIRTMKMRQQASFLPATLTMTVDRGDNVN ISFKKVLIKEEDAVIYKNGSFIHSVPRHEVPDILEVHLPHAQPQDAGVYSARYIGGNLFTSAFTRLIVRR CEAQKWGPDCSRPCTTCKNNGVCHEDTGECICPPGFMGRTCEKACEPHTFGRTCKERCSGPEGCKSYVFC LPDPYGCSCATGWRGLQCNEACPSGYYGPDCKLRCHCTNEEICDRFQGCLCSQGWQGLQCEKEGRPRMTP QIEDLPDHIEVNSGKFNPICKASGWPLPTSEEMTLVKPDGTVLQPNDFNYTDRFSVAIFTVNRVLPPDSG VWVCSVNTVAGMVEKPFNISVKVLPEPLHAPNVIDTGHNFAIINISSEPYFGDGPIKSKKLFYKPVNQAW KYIEVTNEIFTLNYLEPRTDYELCVQLARPGEGGEGHPGPVRRFTTASIGLPPPRGLSLLPKSQTALNLT WQPIFTNSEDEFYVEVERRSLQTTSDQQNIKVPGNLTSVLLSNLVPREQYTVRARVNTKAQGEWSEELRA WTLSDILPPQPENIKISNITDSTAMVSWTIVDGYSISSIIIRYKVQGKNEDQHIDVKIKNATVTQYQLKG LEPETTYHVDIFAENNIGSSNPAFSHELRTLPHSPASADLGGGKMLLIAILGSAGMTCITVLLAFLIMLQ LKRANVQRRMAQAFQNREEPAVQFNSGTLALNRKAKNNPDPTIYPVLDWNDIKFQDVIGEGNFGQVLKAR IKKDGLRMDAAIKRMKEYASKDDHRDFAGELEVLCKLGHHPNIINLLGACEHRGYLYLAIEYAPHGNLLD FLRKSRVLETDPAFAIANSTASTLSSQQLLHFAADVARGMDYLSQKQFIHRDLAARNILVGENYIAKIAD FGLSRGQEVYVKKTMGRLPVRWMAIESLNYSVYTTNSDVWSYGVLLWEIVSLGGTPYCGMTCAELYEKLP QGYRLEKPLNCDDEVYDLMRQCWREKPYERPSFAQILVSLNRMLEERKTYVNTTLYEKFTYAGIDCSAEE AA TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290549
ORF Size	3366 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290549.1, NP_001277478.1
RefSeq Size	4699 bp
RefSeq ORF	3369 bp
Locus ID	21687
UniProt ID	Q02858
MW	126.2 kDa
Gene Summary	Tyrosine-protein kinase that acts as cell-surface receptor for ANGPT1, ANGPT2 and ANGPT4 and regulates angiogenesis, endothelial cell survival, proliferation, migration, adhesion and cell spreading, reorganization of the actin cytoskeleton, but also maintenance of vascular quiescence. Has anti-inflammatory effects by preventing the leakage of proinflammatory plasma proteins and leukocytes from blood vessels. Required for normal angiogenesis and heart development during embryogenesis. Required for post-natal hematopoiesis. After birth, activates or inhibits angiogenesis, depending on the context. Inhibits angiogenesis and promotes vascular stability in quiescent vessels, where endothelial cells have tight contacts. In quiescent vessels, ANGPT1 oligomers recruit TEK to cell-cell contacts, forming complexes with TEK molecules from adjoining cells, and this leads to preferential activation of phosphatidylinositol 3-kinase and the AKT1 signaling cascades. In migrating endothelial cells that lack cell-cell adhesions, ANGT1 recruits TEK to contacts with the extracellular matrix, leading to the formation of focal adhesion complexes, activation of PTK2/FAK and of the downstream kinases MAPK1/ERK2 and MAPK3/ERK1, and ultimately to the stimulation of sprouting angiogenesis. ANGPT1 signaling triggers receptor dimerization and autophosphorylation at specific tyrosine residues that then serve as binding sites for scaffold proteins and effectors. Signaling is modulated by ANGPT2 that has lower affinity for TEK, can promote TEK autophosphorylation in the absence of ANGPT1, but inhibits ANGPT1-mediated signaling by competing for the same binding site. Signaling is also modulated by formation of heterodimers with TIE1, and by proteolytic processing that gives rise to a soluble TEK extracellular domain. The soluble extracellular domain modulates signaling by functioning as decoy receptor for angiopoietins. TEK phosphorylates DOK2, GRB7, GRB14, PIK3R1, SHC1 and TIE1.[UniProtKB/Swiss-Prot Function]

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