Plcb2 (NM_001290790) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	AI550384; B230205M18Rik; B230399N12
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231633 representing NM_001290790 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTTTGCTCAACCCTGTTCTATTGCCCCCTAACGTGAAGGCATATCTGAGCCAAGGGGAGCGCTTCA TCAAGTGGGACGATGAAACTTCAATAGCCTCTCCTGTTATCCTCCGTGTGGATCCCAAGGGCTATTACTT ATACTGGACATATCAAAATCAGAGCCAGAAACTCCGGGAGGTCTTCAACATGGACTTTCCAGACAACCAC TTCCTGCTGAAAACACTCACAGTGGTGTCCGGCCCTGACATGGTGGACCTCACCTTCTACAACTTTGTCT CTTACAAGGAGAACGTAGGCAAGGACTGGGCTGAGGACGTGCTAGCTCTCGCCAAACACCCGATGACAGT CAATGCTCCTCGAAGCACATTCCTGGACAAGATCCTGGTGAAGCTTAAGATGCAGCTCAACCCTGAAGGG AAGATTCCCGTGAAGAATTTTTTCCAGATGTTTCCTGCTGATCGAAAACGGGTGGAAGCTGCCCTCGGTG CTTGTCACCTTGCAAAAGGCAAAAATGATGCTATCAACCCAGAGGACTTCCCAGAATCCGTGTACAAGAG CTTCCTCATGAGCCTCTGTCCTCGGCCAGAAATCGACGAGATCTTCACTTCCTACCACTCTAAAGCTAAG CCCTACATGACCAAGGAACACCTGACCAAATTCATCAATCAGAAGCAGCGAGACCCTCGACTCAACTCCT TGCTGTTCCCACCAGCCCGGCCTGAGCAAGTTCAAGTGCTCATTGACAAGTACGAACCCAGCGGCATCAA TGTGCAGAGGGGCCAGCTGTCACCAGAGGGGATGGTCTGGTTTCTCTGTGGACCAGAGAACAGTGTGCTG GCCCACGATACACTGCTGATCCACCAAGACATGACACAACCACTGAATCACTATTTCATCAACTCCTCAC ACAACACCTACCTGACAGCCGGCCAGTTCTCAGGCCTTTCCTCGGCTGAGATGTACCGCCAGGTGCTGCT GTCTGGCTGCCGGTGTGTAGAGTTAGACTGTTGGAAGGGCAAGCCCCCTGACGAAGAGCCCATTATCACC CACGGCTTCACCATGACCACAGATATCTTGTTCAAGGAAGCAATTGAAGCCATTGCAGAAAGTGCCTTTA AGACCTCCCCGTATCCTGTCATCCTATCATTTGAAAACCATGTAGACTCACCCCGGCAACAGGCTAAGAT GGCTGAGTACTGCAGGAGCATGTTTGGAGAGACCTTGCTCACAGATCCCCTGGAAAATTTCCCTCTGAAA CCTGGCATCCCTCTGCCCAGCCCCGAGGACCTCCGGGGCAAGATCCTCATTAAGAATAAGAAGAACCAGT TTTCTGGGCCAGCATCCCCCAGCAAGAAGCCTGGTGGGGTGGCTGAGGGCAGCCTCCCGTCTAGTGTCCC TGTAGAAGAGGACACGGGGTGGACTGCTGAGGACCGGACTGAGGTGGAGGAGGAGGAGGTGGTGGAAGAG GAGGAAGAGGAGGAGTCAGGAAACCTGGATGAAGAAGAAATTAAGAAGATGCAGTCGGATGAGGGCACAG CAGGCCTGGAGGTGACAGCTTATGAGGAAATGTCCAGCCTTGTCAATTATATCCAGCCCACCAAGTTTAT CTCCTTCGAGTTCTCTGCACAGAAGAACAGAAGTTACGTTGTCTCTTCCTTCACGGAGCTCAAGGCCTAC GAGCTGCTCTCCAAGGCCTCCATGCAGTTTGTGGACTATAATAAACGCCAGATGAGCCGTGTGTACCCCA AGGGCACACGCATGGACTCTTCCAACTACATGCCCCAGATGTTCTGGAATGCTGGATGTCAGATGGTTGC CCTCAATTTCCAGACAATGGACCTGCCCATGCAGCAGAACATGGCACTGTTTGAGTTCAACGGGCAGAGT GGCTACCTCCTAAAGCACGAGTTCATGCGCAGACTGGACAAGCAGTTCAACCCCTTCTCAGTGGACCGCA TTGATGTGGTGGTAGCCACCACCCTTTCCATTACGATCATCTCTGGGCAGTTCCTGTCAGAGCGCAGTGT ACGCACCTATGTGGAAGTGGAACTGTTTGGCCTCCCAGGGGACCCCAAGAGGCGGTATCGAACCAAGCTG TCACCTACTGCTAACTCCATCAATCCTGTCTGGAAAGAGGAACCCTTTATCTTTGAGAAGATCTTGATGC CTGAGCTGGCCTCCCTCAGGATAGCTGTGATGGAAGAAGGCAGCAAATTTCTTGGACACCGCATCATCCC CATCAATGCCTTACATTCTGGATACCACCATCTGTGCCTGCGCAGTGAGAGCAACATGGCCCTTACCATG CCAGCTCTCTTTGTCTTCCTGGAGATGAAGGACTACATACCTGACACCTGGGCAGATCTTACAGTAGCCC TTGCCAACCCCATCAAGTACTTCAATGCCCAGGATAAGAAGTCCGTGAAGCTCAAAGGAGTGACAGGAAG TCTGCCCGAGAAACTCTTCTCTGGGACACCTGTTGCCAGTCAGTCCAATGGGGCGCCAGTCTCGGCAGGC AATGGGTCAACAGCGCCTGGGACCAAGGCCACGGGGGAAGAAGCTACGAAAGAAGTGACAGAGCCACAGA CCGCCAGCTTGGAAGAGTTGCGGGAACTGAAGGGCGTGGTGAAACTGCAGCGAAGACATGAGAAGGAGCT TCGAGAGTTGGAGCGCCGTGGAGCCCGGCGCTGGGAGGAACTGCTGCAGCGTGGCGCCGCACAGCTGGCG GAGCTCCAGACCCAGGCAGCAGGCTGCAAGCTCCGCCCAGGCAAGGGCTCCCGCAAGAAGAGGACCCTGC CCTGTGAGGAGACCGTCGTGGCGCCTAGCGAGCCCCACGACCGGGCGGACCCGCGCGTGCAGGAGCTGAA GGACAGGCTGGAGCAGGAGCTGCAGCAGCAAGGCGAGGAGCAGTACCGCTCCGTCCTCAAGCGCAAGGAG CAGCACGTGACCGAGCAAATCGCCAAGATGATGGAGCTGGCCAGAGAGAAACAGGCTGCTGAACTCAAGA CCTTCAAGGAGACCTCAGAAACTGACACCAAAGAAATGAAGAAAAAACTGGAGGCCAAGAGGCTGGAACG GATCCAAGCCATGACCAAGGTCACCACAGACAAGGTGGCCCAGGAGAGGCTCAAGAGAGAAATTAACAAC TCCCACATCCAGGAAGTGGTCCAGGCTGTCAAGCAGATGACAGAGACCCTGGAGCGTCACCAGGAGAAGC TGGAAGAGAGGCAGACAGCCTGCCTGGAGCAGATCCAGGCAATGGAAAAGCAGTTCCAGGAGAAGGCGCT GGCAGAGTATGAGGCCAAGATGAAGGGCCTGGAGGCTGAAGTAAAGGAGTCGGTGCGGGCCTACTTCAAG GACTGCTTCCCCACCGAAGCAGAAGACAAGCCTGAGAGGTCCTGTGAGGCCTCTGAGGAGTCGTGTCCAC AGGAACCACTTGTGAGCAAGGCAGACACTCAGGAGAGCCGCCTC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231633 representing NM_001290790 Red=Cloning site Green=Tags(s) MSLLNPVLLPPNVKAYLSQGERFIKWDDETSIASPVILRVDPKGYYLYWTYQNQSQKLREVFNMDFPDNH FLLKTLTVVSGPDMVDLTFYNFVSYKENVGKDWAEDVLALAKHPMTVNAPRSTFLDKILVKLKMQLNPEG KIPVKNFFQMFPADRKRVEAALGACHLAKGKNDAINPEDFPESVYKSFLMSLCPRPEIDEIFTSYHSKAK PYMTKEHLTKFINQKQRDPRLNSLLFPPARPEQVQVLIDKYEPSGINVQRGQLSPEGMVWFLCGPENSVL AHDTLLIHQDMTQPLNHYFINSSHNTYLTAGQFSGLSSAEMYRQVLLSGCRCVELDCWKGKPPDEEPIIT HGFTMTTDILFKEAIEAIAESAFKTSPYPVILSFENHVDSPRQQAKMAEYCRSMFGETLLTDPLENFPLK PGIPLPSPEDLRGKILIKNKKNQFSGPASPSKKPGGVAEGSLPSSVPVEEDTGWTAEDRTEVEEEEVVEE EEEEESGNLDEEEIKKMQSDEGTAGLEVTAYEEMSSLVNYIQPTKFISFEFSAQKNRSYVVSSFTELKAY ELLSKASMQFVDYNKRQMSRVYPKGTRMDSSNYMPQMFWNAGCQMVALNFQTMDLPMQQNMALFEFNGQS GYLLKHEFMRRLDKQFNPFSVDRIDVVVATTLSITIISGQFLSERSVRTYVEVELFGLPGDPKRRYRTKL SPTANSINPVWKEEPFIFEKILMPELASLRIAVMEEGSKFLGHRIIPINALHSGYHHLCLRSESNMALTM PALFVFLEMKDYIPDTWADLTVALANPIKYFNAQDKKSVKLKGVTGSLPEKLFSGTPVASQSNGAPVSAG NGSTAPGTKATGEEATKEVTEPQTASLEELRELKGVVKLQRRHEKELRELERRGARRWEELLQRGAAQLA ELQTQAAGCKLRPGKGSRKKRTLPCEETVVAPSEPHDRADPRVQELKDRLEQELQQQGEEQYRSVLKRKE QHVTEQIAKMMELAREKQAAELKTFKETSETDTKEMKKKLEAKRLERIQAMTKVTTDKVAQERLKREINN SHIQEVVQAVKQMTETLERHQEKLEERQTACLEQIQAMEKQFQEKALAEYEAKMKGLEAEVKESVRAYFK DCFPTEAEDKPERSCEASEESCPQEPLVSKADTQESRL TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001290790
ORF Size	3474 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001290790.1, NP_001277719.1
RefSeq Size	5091 bp
RefSeq ORF	3477 bp
Locus ID	18796
UniProt ID	A3KGF7
MW	132.3 kDa
Gene Summary	The production of the second messenger molecules diacylglycerol (DAG) and inositol 1,4,5-trisphosphate (IP3) is mediated by activated phosphatidylinositol-specific phospholipase C enzymes. This protein may be involved in the transduction of bitter taste stimuli (By similarity).[UniProtKB/Swiss-Prot Function]

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