Cadps2 (NM_001252105) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	A230044C21Rik; Caps-2; Caps2; Cpd2
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231741 representing NM_001252105 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTCGACCCGTCTTCCAGCGAGGAGGAGTCGGACGAGGGGCTGGAGGAGGAGAGCCGCGAGGTGTTGG TGGCGCCGGGCGTCTCCCAGCGAGCGCCGCCGGCCGCGGCTCGGGAAGGGCGGCGGGACGCGCCGGGACG CTCCGGTGGTGGCAGCGGCGGCGGAGCGGCCAGACCCGTGAGCCCGAGCCCCTCGGTACTCAGCGAGGGG CGAAATGAGCCCGAGCTGCAGCTGGACGAGGAGCAGGAGCGGCGCATCCGCCTGCAGCTCTACGTCTTCG TGGTGAGGTGCATCGCGTACCCATTCAACGCCAAGCAGCCCACCGACATGGCCCGGAGGCAGCAGAAGCT TAACAAACAACAATTGCAGTTACTGAAAGAACGGTTCCAGGCCTTTCTCAATGGAGAAACTCAGATAGTA GCAGACGAAGCCTTCTGCAATGCCGTCCGGAGTTACTATGAGGTGTTTCTAAAGAGTGACCGTGTGGCCA GAATGGTTCAAAGTGGAGGATGCTCTGCTAATGATTTCAGAGAGGTGTTTAAGAAGAACATAGAAAAACG TGTCCGTAGCCTGCCAGAGATAGATGGCCTGAGCAAAGAGACAGTGCTGAGCTCATGGATAGCCAAATAT GACGCCATTTACAGGGGAGAAGAAGATTTGTGCAAACAGCCGAACAGGATGACCCTCAGTGCCGTGTCTG AGCTTATTCTGAGTAAGGAGCAGCTCTATGAAATGTTCCAGCAGATTCTGGGCATTAAGAAGCTGGAACA CCAGCTACTTTATAACGCATGCCAGCTGGATAACGCTGATGAACAAGCAGCCCAGATCAGAAGGGAACTT GATGGCCGCCTGCAGTTGGCAGAGAAGATGGCAAAGGAGAGAAGATTCCCCAGATTCATCTCGAAAGAAA TGGAGAGTATGTACATTGAAGAGCTGCGAGCCTCCGTGAACCTGCTAATGGCCAATTTAGAAAGTCTTCC AGTTTCAAAAGGTGGCCCGGAATTTAAATTACAAAAATTAAAGCGTTCGCAGAACTCTGCGTTTCTGGAC CTGGGAGATGAGAACGAGATCCAGCTGTCCAAGTCGGATGTGGTGTTGTCGTTCACGTTAGAGATTGTCA TCATGGAAGTGCAAGGACTGAAATCTGTGGCTCCCAATCGAATCGTTTACTGCACAATGGAGGTGGAAGG AGGAGAAAAACTCCAGACAGACCAGGCTGAAGCATCAAGGCCACAATGGGGGACCCAAGGAGATTTCAAC ACTACCCACCCTCGGCCTGTCGTCAAAGTGAAGCTCTTCACAGAAAGCACGGGGGTCCTGGCCCTGGAAG ACAAGGAACTGGGCAGGGTGGTGTTATACCCAACTTCTAATAGCTCCAAGTCAGCAGAGTTACACCGAAT GACAGTACCCAAGAACAGTCAGGACTCGGACCTAAAGATCAAATTGGCAGTGCGCATGGATAAACCAGCA CACATGAAGCATAGTGGGTACCTGTATGCCCTTGGACAGAAGGTTTGGAAAAGATGGAAAAAGCGTTACT TTGTTCTCGTTCAGGTTAGCCAGTACACCTTTGCTATGTGCAGCTATAGAGAAAAAAAGTCGGAACCACA GGAATTAATGCAACTGGAAGGATACACAGTGGATTACACAGACCCTCACCCAGGCCTTCAGGGTGGTCAG GTGTTCTTCAACGCTGTTAAAGAGGGAGATACTGTGATCTTTGCCAGTGATGATGAACAGGACAGAATAT TATGGGTACAAGCCATGTACAGGGCTACAGGCCAGTCTTACAAACCAGTTCCTGCAGTCCAAAGCCAGAA GCTGAATCCTAAAGGCGGAGCTCTCCATGCAGATGCTCAGCTTTATGCAGACCGTTTTCAGAAACACGGG ATGGATGAGTTTATTTCTGCGAGTCCTTGCAAGCTTGACCATGCCTTCCTCTTCAGAATTCTCCAGAGAC AGACTTTGGATCACAGACTGAATGATTCGTATTCTTGTTTGGGGTGGTTTAGCCCTGGCCAAGTCTTTGT GTTAGATGAGTACTGTGCCCGCTACGGAGTGAGAGGCTGTCACAGGCATCTCTGCTACCTTACAGAACTG ATGGAACATTCAGAAAACGGTGCTGTCATTGACCCCACCCTGCTCCATTACAGCTTTGCATTCTGTGCCT CTCACGTGCACGGCAACAGGCCTGATGGGATTGGAACGGTTTCAGTGGAAGAGAAAGAAAGATTTGAGGA GATAAAAGACCGACTTTCTTCGCTTTTAGAAAACCAGATCAGCCACTTCAGATACTGCTTTCCCTTCGGA CGACCTGAGGGTGCCCTAAAAGCTACGCTCTCCTTACTTGAAAGGGTTTTAATGAAAGACATTGCCACTC CTATCCCTGCGGAGGAGGTGAAGAAAGTGGTCAGAAAATGTCTGGAGAAAGCTGCCTTGATCAATTACAC TAGGCTCACAGAATATGCCAAAATAGAAGGGCCCGCAGAAAAGGAAACAGAGACCATGAACCAGGCAACT CCTGCCAGGAAGCTGGAAGAGGTTCTTCATCTTGCAGAGCTCTGCATAGAAGTCCTACAGCAAAATGAGG AGCATCATGCTGAGGGAAGAGAGGCATTTGCCTGGTGGCCTGACTTGTTGGCCGAGCATGCAGAGAAGTT TTGGGCTTTATTCACAGTAGACATGGATACTGCGCTGGAGGCCCAACCTCAAGACTCCTGGGATAGCTTT CCCCTTTTCCAGCTGCTTAATAATTTCCTCAGAAATGACACACTTTTGTGTAATGGAAAATTCCACAAGC ACTTGCAAGAAATCTTTGTGCCCTTGGTTGTCCGCTACGTTGACCTGATGGAGTCTGCCATCGCCCAGTC CATTCACAGAGGTTTTGAGCAGGAGACATGGCAGCCTGTCAACAATGGCTCAACAACTTCCGAGGATCTG TTCTGGAAGCTCGATGCGCTGCAAATGTTCGTCTTTGATCTCCATTGGCCAGAACAAGAGTTTGCCCACC ACTTAGAGCAAAGACTTAAACTAATGGCCAGTGATATGATAGAGGCGTGTGTCAAAAGAACAAGAACTGC GTTCGAACTCAAGCTACAAAAGGCAAACAAAACAACTGACTTGCGCATCCCAGCTTCCGTGTGCACAATG TTTAATGTATTAGTTGATGCTAAAAAGCAAAGCACCAAGCTGTGTGCCCTGGATGGAGGACAAGAGCAAC AGTACCATTCAAAAATAGATGATTTGATTGACAACACCGTGAAAGAAATCATTGCACTGCTGGTTTCAAA GTTTGTTTCAGTGTTGGAAGGGGTGCTTTCGAAGTTGTCGAGGTATGACGAAGGCACTTTCTTCTCATCC ATCCTGTCCTTCACTGTGAAAGCAGCTGCAAAATATGTGGATGTCCCTAAACCAGGAATGGATCTGGCAG ACACCTACATTATGTTTGTCCGGCAAAACCAGGATATTCTTCGAGAAAAAGTCAATGAAGAGATGTACAT AGAAAAGTTGTTTGATCAATGGTACAGCAATTCCATGAAAGTCATTTGTGTGTGGCTGGCTGACAGACTA GACCTCCAGCTTCATATTTACCAACTGAAGACGCTCATCAAGATTGTGAAGAAAACCTATAGGGATTTCC GATTGCAGGGTGTGTTGGAGGGGACGCTGAACAGTAAGACATATGATACTCTGCACAGACGTCTAACTGT AGAGGAGGCCACAGCCTCTGTCTCAGAAGGCGGAGGACTTCAGGGCATTACCATGAAGGACAGTGATGAG GAGGAAGAAGGC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231741 representing NM_001252105 Red=Cloning site Green=Tags(s) MLDPSSSEEESDEGLEEESREVLVAPGVSQRAPPAAAREGRRDAPGRSGGGSGGGAARPVSPSPSVLSEG RNEPELQLDEEQERRIRLQLYVFVVRCIAYPFNAKQPTDMARRQQKLNKQQLQLLKERFQAFLNGETQIV ADEAFCNAVRSYYEVFLKSDRVARMVQSGGCSANDFREVFKKNIEKRVRSLPEIDGLSKETVLSSWIAKY DAIYRGEEDLCKQPNRMTLSAVSELILSKEQLYEMFQQILGIKKLEHQLLYNACQLDNADEQAAQIRREL DGRLQLAEKMAKERRFPRFISKEMESMYIEELRASVNLLMANLESLPVSKGGPEFKLQKLKRSQNSAFLD LGDENEIQLSKSDVVLSFTLEIVIMEVQGLKSVAPNRIVYCTMEVEGGEKLQTDQAEASRPQWGTQGDFN TTHPRPVVKVKLFTESTGVLALEDKELGRVVLYPTSNSSKSAELHRMTVPKNSQDSDLKIKLAVRMDKPA HMKHSGYLYALGQKVWKRWKKRYFVLVQVSQYTFAMCSYREKKSEPQELMQLEGYTVDYTDPHPGLQGGQ VFFNAVKEGDTVIFASDDEQDRILWVQAMYRATGQSYKPVPAVQSQKLNPKGGALHADAQLYADRFQKHG MDEFISASPCKLDHAFLFRILQRQTLDHRLNDSYSCLGWFSPGQVFVLDEYCARYGVRGCHRHLCYLTEL MEHSENGAVIDPTLLHYSFAFCASHVHGNRPDGIGTVSVEEKERFEEIKDRLSSLLENQISHFRYCFPFG RPEGALKATLSLLERVLMKDIATPIPAEEVKKVVRKCLEKAALINYTRLTEYAKIEGPAEKETETMNQAT PARKLEEVLHLAELCIEVLQQNEEHHAEGREAFAWWPDLLAEHAEKFWALFTVDMDTALEAQPQDSWDSF PLFQLLNNFLRNDTLLCNGKFHKHLQEIFVPLVVRYVDLMESAIAQSIHRGFEQETWQPVNNGSTTSEDL FWKLDALQMFVFDLHWPEQEFAHHLEQRLKLMASDMIEACVKRTRTAFELKLQKANKTTDLRIPASVCTM FNVLVDAKKQSTKLCALDGGQEQQYHSKIDDLIDNTVKEIIALLVSKFVSVLEGVLSKLSRYDEGTFFSS ILSFTVKAAAKYVDVPKPGMDLADTYIMFVRQNQDILREKVNEEMYIEKLFDQWYSNSMKVICVWLADRL DLQLHIYQLKTLIKIVKKTYRDFRLQGVLEGTLNSKTYDTLHRRLTVEEATASVSEGGGLQGITMKDSDE EEEG TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001252105
ORF Size	3792 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001252105.1, NP_001239034.1
RefSeq Size	4849 bp
RefSeq ORF	3795 bp
Locus ID	320405
UniProt ID	Q8BYR5
MW	144.7 kDa
Gene Summary	Calcium-binding protein involved in exocytosis of vesicles filled with neurotransmitters and neuropeptides. Probably acts upstream of fusion in the biogenesis or maintenance of mature secretory vesicles. Regulates neurotrophin release from granule cells leading to regulate cell differentiation and survival during cerebellar development. May specifically mediate the Ca(2+)-dependent exocytosis of large dense-core vesicles (DCVs) and other dense-core vesicles.[UniProtKB/Swiss-Prot Function]

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