Wnk1 (NM_001199083) Mouse Tagged ORF Clone

Specifications

Product Data
Type	Mouse Tagged ORF Clone
Tag	Myc-DDK
Synonyms	6430573H23Rik; EG406236; Hsn2; mKIAA0344; Prkwnk1
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >MR231966 representing NM_001199083 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTCTGACGGTGCCGCCGAGAAGCAAAGCGGCACTCCGGGCTTCCTTACGCCTCCGGCGCCCGTCCCCA AGAATGGCTCAAGCTCCGATTCTTCCGTGGGCGAGAAACTGGGGGCTACGGTCGCCGACTCGGGAGTTGG CAGGACGGAGGAATATCGGCGCCGCCGGCACACTATGGACAAGGACAGTCGTGGGGCGGCCGCCACCACC ACCCCCACGGAGCACCGATTCTTCCGCCGAAGCGTCATCTGCGACTCCAATGCCACCGCGTTGGAGCTGC CGGGCCTCCCTCTCTCTATCCCGCAACCTAGTGTACCTGCAGTGGTGCCCCAGAGTGCTCCACCCGAACC TCACAGAGAGGAAACTTTGACCGCCACCGTTGCTTCCCAGGTGTCTCAGCAACCCTCGGCTGCTGCCTCC CCTGGGGAACAAGCCGTTGTAGGCTCGGCCACCACGACCGTCCCCAGCAGTACCAGCAAAGACCGCCCGG TGTCCCAGCCCAGCCTCGTGGGGAGCAAAGAGGAGCCACCGCCGTCCAGAAGTGGAAGTGGCAGTGGTGG CGCCAGCGCCAAAGAGGCGCAGGAGGATAGGAGCCAGCAACAGGATGATATCGAAGAGCTGGAGACCAAG GCAGTGGGAATGTCCAATGACGGTCGCTTTCTCAAATTTGACATCGAAATCGGCAGAGGCTCCTTTAAGA CAGTCTACAAAGGTCTGGACACCGAAACCACTGTGGAAGTCGCCTGGTGTGAATTGCAGGATCGAAAGTT AACAAAATCTGAAAGGCAGAGATTCAAAGAAGAGGCTGAAATGTTAAAGGGTCTTCAACATCCCAACATT GTTCGATTCTATGATTCCTGGGAATCCACAGTAAAAGGGAAAAAATGCATTGTTTTAGTGACTGAACTAA TGACATCTGGAACACTTAAGACGTACTTAAAAAGGTTTAAAGTGATGAAAATCAAAGTTTTAAGAAGCTG GTGTCGGCAAATCCTTAAAGGGCTTCAGTTTCTCCACACACGAACTCCACCAATTATTCACCGGGATCTT AAATGTGACAACATCTTTATCACTGGTCCTACTGGCTCAGTCAAGATCGGAGACCTTGGTCTGGCAACTC TAAAGCGGGCTTCTTTTGCCAAGAGTGTGATAGGTACCCCAGAGTTTATGGCTCCTGAGATGTATGAGGA GAAATATGATGAATCCGTTGATGTTTATGCTTTTGGGATGTGCATGCTTGAGATGGCTACATCTGAATAT CCATACTCAGAATGCCAAAATGCTGCGCAGATCTACCGTCGAGTGACCAGTGGAGTGAAGCCAGCCAGTT TTGACAAAGTAGCAATTCCTGAAGTAAAAGAAATTATTGAAGGATGTATCCGACAAAACAAAGATGAAAG ATATTCCATCAAAGACCTTTTGAACCATGCCTTTTTCCAGGAGGAAACAGGGGTACGGGTAGAATTAGCA GAAGAAGATGATGGAGAAAAGATAGCTATTAAATTATGGCTGCGTATTGAAGATATTAAGAAATTAAAGG GAAAATACAAAGACAATGAAGCTATTGAGTTTTCCTTTGACTTGGAGAGGGATGTACCAGAAGATGTTGC TCAAGAAATGGTTGAGTCTGGTTATGTCTGTGAAGGTGATCACAAGACCATGGCTAAAGCTATCAAAGAT AGGGTGTCCTTAATTAAGAGAAAACGAGAGCAGCGGCAGTTGGTTCGAGAAGAGCAAGAAAAAAGAAAAC AAGAAGAGAGCAGTTTCAAACAGCAGAATGAACAACAAGCAAGCGTTTCCCAGGCAGGAATCCAGCAGCT CTCTGCTGCTAGCACTGGCATACCCACTGCTCCTGCCACCTCTGCTTCAGTTTCTACACAGGTAGAACCT GAAGAACCTGAGGCAGATCAACATCAACAGCTGCAATATCAGCAACCTAGTATATCTGTGTTGTCTGATG GAACCATTGATAGTGGTCAAGGATCATCCGTATTCACAGAATCCAGAGTGAGCAGCCAACAGACTGTTTC CTATGGTTCCCAACATGAGCAGGCACATTCTACTGGCACAGCACCAGGACATACAGTTTCTAGTATCCAA GCACAATCTCAGCCCCATGGGGTGTATCCACCCTCAAGTATGCCTCGTCGTGGCCGTAGCATGTCGGTTT GTGTTCCCCATCTTTCTGCTGTTCCCTCTCTATCCCGCATCTCTCCCAGTGCTCCTTCCACCCCACCACC AGTGCTGTCTGCACCTCTTTGTCCTTCCCTCCTTCGGACTGCCCCTGAGGAAACTTTTGCCGAAAAACTC TCTAAAGCATTGGAGAGTGTCCTGCCTATGCACTCTGCCTCCCAGCGCAAGCACCGACGCTCCAGCCTGC CTTCCCTCTTTGTCACTACTCCTCAGTCCATGGCGCATCCGTGTGGGGGGACCCCAACATACCCAGAATC ACAGATATTTTTCCCAACTATTCATGAACGTCCAGTTTCTTTTTCACCACCTCCCACCTGTCCACCGAAA GTAGCCATTTCCCAACGACGTAAGAGCACCTCCTTCCTGGAAGCCCAAACTCGCCACTTCCAACCCCTGC TGAGGACTGTTGGCCAAAATCATCTTCCACCTGGTAGCAGCCCAACTAACTGGACACCAGAGGCCATAGT TATGTTGGGTGCTACAGCCAATAGAGTAAATAGAGAGCTATGTGAGATGCAGGTCCAACCTGTGTTTGAG CCAACCCAGATTTACAGTGACTATAGACCTGGACTAGTACTGGCAGAAGAAGCTCACTATTTTATTCCTC AGGAAACAGTATATCTAGCTGGGGTGCATTACCAGGCCCAGGTGGCAGGACAGTATGAGGGCATTTCATA CAACTCACCAGTACTGTCAAGTCCTATGAAACAGATATCTGAACAGAAGCCAGTGCCGGGGGGCCCTGCC TCAAGTTCTGTCTTTGAATTTCCTTCTGGACAGGCTTTCCTGGTAGGACACCTTCAGAATTTAAGATTAG ATTCTGGACCAAGTCCAGCATCACCCCTCTCTAGTATTTCTGCGCCTAACAGTACAGATGCTACACATCT GAAATTTCACCCTGTCTTTGTACCTCATTCTGCACCAGCTGTGTTAACTAATAGCAATGAGAACAGAAGC AACTGTGTATTTGAATTTCATGCTCAAACACCAAGTTCTTCAGGAGAAGGAGGTGGGATTTTACCTCAAC GTGTTTACCGAAATCGGCAGGTTGCAGTGGACTCAAATCAGGAAGAACTGTCTCCTCAGTCAGTTGGATT ACATTGCCACCTGCAGCCTGTTACTGAAGAACAGCGTAATAACCATGCCCCCGAATTGACCATTTCTGTG GTAGAACCTATGGGACAAATCTGGCCAATAGGAAGCCCAGAATATTCCAGTGATTCCTCTCAAATTACTT CTTCAGATCTCAGTGATTTTCAATCACCTCCCCCTACAGGGGGGACAGCTGCACCTTTTGGCTCTGACGT CTCATTGCCCTTTATTCGCCTGCCTCAGACAGTGTTACAAGAATCCCCACTATTCTTCTGTTTCCCCCAA GGAACCACATCTCAGCAGGTCTTATCTGCTTCATATTCTTCAGGAGGATCTACACTCCATCCACAGGCAC AGGGGCAGAACCAGGGCCAGCCATCAAGTAGTTTGGCTGGGGTTCTATCTTCACAACCTATCCAACATCC TCAGCAGCAGGGAATACAGCCAACTGTTCCTTCTCAACAGGCAGTACAGTATTCACTTCCACAAGCAGCA TCTTCCAGTGAAGGTACTACTGCACAGCCAGTGAGTCAGCCTCAAGTGTCAGCTGGAACACAGAGTACTC AGGGAGTCTCTCAGGCTGCTCCTCCAGAACAAACTCCAATAACACAGTCACAACCTACTCAGCCTGTTCC ATTGGTTACCTCTGCAGACAGTGCACATTCAGACGTTGCTTCTGGTATGAGTGATGGCAATGAGAATGCT CCATCATCCAGCGGGAGGCATGAAGGGAGGACAACAAAACGGCATTATAGAAAATCCGTAAGAAGTCGCT CTCGGCATGAAAAGACTTCTCGCCCAAAACTGAGGATTTTAAATGTTTCAAATAAAGGAGACCGAGTAGT AGAATGTCAATTAGAGACTCATAATCGGAAGATGGTTACATTTAAATTTGACCTAGATGGTGACAACCCT GAAGAGATAGCAACAATTATGGTGAACAATGACTTTATTCTAGCAATAGAGAGAGAGTCTTTTGTGGCTC AAGTGCGGGAAATTATTGAAAAGGCTGATGAAATGTTAAGTGAGGATGTCAGTGTGGAGCCTGAGGGTGA CCAGGGATTGGAGAGTCTGCAAGGAAAGGATGACTATGGCTTCCCAGGTTCTCAGAAATTAGAAGGAGAG TTCAAGCAACCAATTGCTGTATCTTCCATGCCACAACAAATAGGTGTTCCTACCAGTTCTTTAACGCAAG TTGTTCATTCTGCTGGAAGACGGTTTATAGTGAGTCCTGTACCTGAAAGTAGGTTACGAGAATCAAAAGT TTTCACCAGTGACATATCAGATCCAGTTGTGGCCTCTACATCTCAGGCCCCTGGAATGAACTTATCTCAC TCTGCTTCATCCCTCAGTCTCCAGCAGGCTTTTTCTGAACTGAAACATGGTCAGATGACTGAAGGACCCA ATACAGCACCTCCAAATTTTAATCACATGGCGGGACCAACATTTTCTCCCTTTTTGGCTAGCATTGCTGG TGTCCAAACTGTAGCAGCATCCACACCGTCAGTATCAGTCCCTATAACAAGCAGTCCTCTTAATGACATT TCCACATCAGTGATGCAGTCTGAGACTGCACTGCCCACTGAAAAAGGCATCGTGGGAGTTACCACTACCA GCACAGGTGTGGTAGCTTCAGGTGGTCTCACCACAATGTCTGTGTCTGAGTCACCAACATCTTCCAGTGC GGTTTCAAGTTCTACAGTACCTGCAGTTGTCACTGTGTCCACACCATCCCAGCCAGTTCAGGCTTCCACA TCTGGGAGCATTGCTTCAAGTACAGGCAGTTTTCCTCCTGGAACATTTTCTACAACTACAGCTACTACAA TGGGTAGTGTGGTGGCCCCAGATGCTAAGCCTCCAACTGTATTATTGCAACAGGTAGCAAGCAATACTGC TGGGGTTGCCATAGTGACATCAGTCTCTACCACCACTCCATTCCCAGGCATGGCTTCACAGCCATCCCTT CCACTTAGTAGCAGCACCTCTGCTCCTACTCTAGCTGAAACAATGGTGGTTAGTGCACACTCACTAGACA AGGCATCTCACAGTAGTACAGCAGGATTGGGTTTGTCCTTTTGTGCGCCATCCTCTTCCTCCTCCTCTGG AACAGCTGTGTCTACTTCTGTTTCTCAGCCTGGTATGGTTCATCCTTTGGTCATCTCATCAGCTGTAGTT TCTACACCTGGCCTCCCCCAACCAGTAGTACCTACTTCTACACCTTTATTACCCCAAGTACCTAATATCC CACCCTTGGTACAACCTGTTGTTAATGTGCCTGCTGTACAGCAAACACTCATTCACAGTCAGCCTCAACC AGCTCTGCTTCCCAACCAGCCTCACACACACTGTCCTGAAATGGATGCTGATACCCAATCCAAAGCTCCT GGGATTGATGATATAAAGACTCTGGAGGAAAAGCTGAGATCTCTCTTTAGTGAACACAGCTCATCTGGAA CTCAGCATGCTTCTGTCTCCCTAGAGACACCTTTAGTAGTAGAGACGACTGTCACACCGGGCATCACTAC CACTGCTGTAGCACCAAGTAAACTCATGACTTCCACTACAAGTACTTGCTTACCACCAACCAGTTTACCA TTAGGAGCAGCTGGCATGCCAGTTATGCCAGTAGGCACACCTGGGCAGGTTTCTACCCCAGGCACACACG CTTCAGCCCCAGTCGGCACTGCAACAGGAGTAAAACCTGGAACAACTCCCCCAAAGCCAACTAAGACTGT GGTGCCACCAGTGGGTACTGAACTTTCAGCCGGTACTGTACCCTGTGAGCAGCTGCCACCTTTTCCTGGA CCTTCTCTAATTCAGTCCCAGCAACCACTAGAAGATCTTGATGCTCAGTTGAGAAGAACACTTAGTCCAG AGACTATTACAGTGGCACCTGCAGTTGGTCCTTTGTCTACGATGTCTTCAACAACAGTTACAGAAGCAGG GACTCGGCTTCAAAAGGATGGCACAGAAGGTCATGTCACAGCAACTAGTTCAGGAGCTGGTGTTGTTAAG ATGGGGCGATTTCAGGTTTCAGTTACAATGGATGATGCCCAGAAAGAACGTAAAAATAGGTCAGAAGATA CAAAGTCGGTTCATTTTGAATCTAGCACATCAGAGTCATCAGTTCTTTCAAGTAGTAGTCCAGAGAGTAC CCTGGTAAAGCCAGAGCCTAATGGAATTAGTATTTCTGGCATCTCCTTAGATGTCCCAGACAGCACCCAC AAAGCACCTACCCCAGAAGCAAAGTCAGATGCAGGACAGCCTACCAAGGTGGGACGTTTTCAGGTGACAA CTACAGCCAACAAAGTGGGTCGTTTCTCTGTTTCAAGAACTGAGGACAAGGTCACAGAGCTCAAAAAAGA GGGACCAGTGACCTCTCCTCCTTTCAGAGATTCAGAACAAACTGTTATTCCTGCTGTGATACCAAAGAAG GAGAAGCCTGAGCTGGCAGAACCTTCCCATTTGAATGGGCCATCTTCAGACCTGGAGGCTGCCTTTCTAA GTAGGGGTACAGAGGATGGCTCAGGTAGTCCACACTCTCCCCCTCACCTGTGTTCAAAGAGCCTTCCCGT CCAGAATCTAAGTCAAAGCCTTAGTAATTCATTCAACTCCTCATACATGAGTAGTGACAATGAGTCCGAC ATTGAAGATGAAGACTTAAGGTTAGAACTACGACGACTACGAGAAAAACATCTTAAAGAGATTCAGGACC TGCAGAGTCGCCAGAAGCATGAAATTGAATCTTTGTATACTAAACTGGGCAAGGTTCCCCCTGCTGTCAT TATTCCCCCAGCTGCTCCTCTGTCGGGGAGAAGAAGGAGACCCACTAAAAGCAAAGGCAGCAAGTCTAGT CGCAGCAGCTCATTGGGCAATAAAAGCCCACAGCTTTCAGGCAACCTGTCTGGTCAGAGTGGAACTTCAG TCTTACACCCCCAACAGACCCTCCATCCCGCAGGAAACACCCCCGAGACTGGGCACAACCAGCTGTTACA GCCTCTTAAGCCATCTCCCTCCAGTGACAACCTCTATTCAGCCTTTACCAGTGATGGTGCCATTTCAGTA CCAAGCCTTTCTGCTCCAGGTCAAGGGACCAGCAGCACAAATACCGTTGGAGGAACAGTGAGTAGCCAGG CTGCCCAGGCTCAGCCTCCTGCTATGACTTCCAGTAGGAAGGGCACATTCACAGATGACCTGCACAAGTT AGTAGACAACTGGGCCCGAGATGCCATGAATCTTTCAGGCAGGAGAGGCAGCAAAGGACACATGAATTAT GAGGGCCCTGGAATGGCAAGGAAGTTCTCTGCTCCTGGGCAGCTGTGTGTTCCCATGACCTCAAACCTGG GTGGCTCTACCCCCATCTCTGCAGCATCTGCTACCTCTCTAGGTCACTTCACCAAATCCATGTGTCCCCC ACAGCAGTATGGTTTTCCACCTGCCCCATTTGGCACTCAGTGGAGTGGAACAGGTGGTCCAGCACCGCAG CCACTTGGCCAGTTCCAACCTGTAGGAACAGCATCCTTGCAAAATTTCAACATCAGCAATTTGCAGAAGT CCATCAGCAACCCCCCAGGTTCCAATCTACGGACCACC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >MR231966 representing NM_001199083 Red=Cloning site Green=Tags(s) MSDGAAEKQSGTPGFLTPPAPVPKNGSSSDSSVGEKLGATVADSGVGRTEEYRRRRHTMDKDSRGAAATT TPTEHRFFRRSVICDSNATALELPGLPLSIPQPSVPAVVPQSAPPEPHREETLTATVASQVSQQPSAAAS PGEQAVVGSATTTVPSSTSKDRPVSQPSLVGSKEEPPPSRSGSGSGGASAKEAQEDRSQQQDDIEELETK AVGMSNDGRFLKFDIEIGRGSFKTVYKGLDTETTVEVAWCELQDRKLTKSERQRFKEEAEMLKGLQHPNI VRFYDSWESTVKGKKCIVLVTELMTSGTLKTYLKRFKVMKIKVLRSWCRQILKGLQFLHTRTPPIIHRDL KCDNIFITGPTGSVKIGDLGLATLKRASFAKSVIGTPEFMAPEMYEEKYDESVDVYAFGMCMLEMATSEY PYSECQNAAQIYRRVTSGVKPASFDKVAIPEVKEIIEGCIRQNKDERYSIKDLLNHAFFQEETGVRVELA EEDDGEKIAIKLWLRIEDIKKLKGKYKDNEAIEFSFDLERDVPEDVAQEMVESGYVCEGDHKTMAKAIKD RVSLIKRKREQRQLVREEQEKRKQEESSFKQQNEQQASVSQAGIQQLSAASTGIPTAPATSASVSTQVEP EEPEADQHQQLQYQQPSISVLSDGTIDSGQGSSVFTESRVSSQQTVSYGSQHEQAHSTGTAPGHTVSSIQ AQSQPHGVYPPSSMPRRGRSMSVCVPHLSAVPSLSRISPSAPSTPPPVLSAPLCPSLLRTAPEETFAEKL SKALESVLPMHSASQRKHRRSSLPSLFVTTPQSMAHPCGGTPTYPESQIFFPTIHERPVSFSPPPTCPPK VAISQRRKSTSFLEAQTRHFQPLLRTVGQNHLPPGSSPTNWTPEAIVMLGATANRVNRELCEMQVQPVFE PTQIYSDYRPGLVLAEEAHYFIPQETVYLAGVHYQAQVAGQYEGISYNSPVLSSPMKQISEQKPVPGGPA SSSVFEFPSGQAFLVGHLQNLRLDSGPSPASPLSSISAPNSTDATHLKFHPVFVPHSAPAVLTNSNENRS NCVFEFHAQTPSSSGEGGGILPQRVYRNRQVAVDSNQEELSPQSVGLHCHLQPVTEEQRNNHAPELTISV VEPMGQIWPIGSPEYSSDSSQITSSDLSDFQSPPPTGGTAAPFGSDVSLPFIRLPQTVLQESPLFFCFPQ GTTSQQVLSASYSSGGSTLHPQAQGQNQGQPSSSLAGVLSSQPIQHPQQQGIQPTVPSQQAVQYSLPQAA SSSEGTTAQPVSQPQVSAGTQSTQGVSQAAPPEQTPITQSQPTQPVPLVTSADSAHSDVASGMSDGNENA PSSSGRHEGRTTKRHYRKSVRSRSRHEKTSRPKLRILNVSNKGDRVVECQLETHNRKMVTFKFDLDGDNP EEIATIMVNNDFILAIERESFVAQVREIIEKADEMLSEDVSVEPEGDQGLESLQGKDDYGFPGSQKLEGE FKQPIAVSSMPQQIGVPTSSLTQVVHSAGRRFIVSPVPESRLRESKVFTSDISDPVVASTSQAPGMNLSH SASSLSLQQAFSELKHGQMTEGPNTAPPNFNHMAGPTFSPFLASIAGVQTVAASTPSVSVPITSSPLNDI STSVMQSETALPTEKGIVGVTTTSTGVVASGGLTTMSVSESPTSSSAVSSSTVPAVVTVSTPSQPVQAST SGSIASSTGSFPPGTFSTTTATTMGSVVAPDAKPPTVLLQQVASNTAGVAIVTSVSTTTPFPGMASQPSL PLSSSTSAPTLAETMVVSAHSLDKASHSSTAGLGLSFCAPSSSSSSGTAVSTSVSQPGMVHPLVISSAVV STPGLPQPVVPTSTPLLPQVPNIPPLVQPVVNVPAVQQTLIHSQPQPALLPNQPHTHCPEMDADTQSKAP GIDDIKTLEEKLRSLFSEHSSSGTQHASVSLETPLVVETTVTPGITTTAVAPSKLMTSTTSTCLPPTSLP LGAAGMPVMPVGTPGQVSTPGTHASAPVGTATGVKPGTTPPKPTKTVVPPVGTELSAGTVPCEQLPPFPG PSLIQSQQPLEDLDAQLRRTLSPETITVAPAVGPLSTMSSTTVTEAGTRLQKDGTEGHVTATSSGAGVVK MGRFQVSVTMDDAQKERKNRSEDTKSVHFESSTSESSVLSSSSPESTLVKPEPNGISISGISLDVPDSTH KAPTPEAKSDAGQPTKVGRFQVTTTANKVGRFSVSRTEDKVTELKKEGPVTSPPFRDSEQTVIPAVIPKK EKPELAEPSHLNGPSSDLEAAFLSRGTEDGSGSPHSPPHLCSKSLPVQNLSQSLSNSFNSSYMSSDNESD IEDEDLRLELRRLREKHLKEIQDLQSRQKHEIESLYTKLGKVPPAVIIPPAAPLSGRRRRPTKSKGSKSS RSSSLGNKSPQLSGNLSGQSGTSVLHPQQTLHPAGNTPETGHNQLLQPLKPSPSSDNLYSAFTSDGAISV PSLSAPGQGTSSTNTVGGTVSSQAAQAQPPAMTSSRKGTFTDDLHKLVDNWARDAMNLSGRRGSKGHMNY EGPGMARKFSAPGQLCVPMTSNLGGSTPISAASATSLGHFTKSMCPPQQYGFPPAPFGTQWSGTGGPAPQ PLGQFQPVGTASLQNFNISNLQKSISNPPGSNLRTT TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001199083
ORF Size	7878 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001199083.1, NP_001186012.1
RefSeq Size	11319 bp
RefSeq ORF	7881 bp
Locus ID	232341
UniProt ID	P83741
MW	279.6 kDa
Gene Summary	Serine/threonine kinase which plays an important role in the regulation of electrolyte homeostasis, cell signaling, survival, and proliferation. Acts as an activator and inhibitor of sodium-coupled chloride cotransporters and potassium-coupled chloride cotransporters respectively. Activates SCNN1A, SCNN1B, SCNN1D and SGK1. Controls sodium and chloride ion transport by inhibiting the activity of WNK4, by either phosphorylating the kinase or via an interaction between WNK4 and the autoinhibitory domain of WNK1. WNK4 regulates the activity of the thiazide-sensitive Na-Cl cotransporter, SLC12A3, by phosphorylation. WNK1 may also play a role in actin cytoskeletal reorganization. Phosphorylates NEDD4L. Acts as a scaffold to inhibit SLC4A4, SLC26A6 as well as CFTR activities and surface expression, recruits STK39 which mediates the inhibition (PubMed:21317537, PubMed:23542070).[UniProtKB/Swiss-Prot Function]

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