PPP4C (NM_002720) Human Tagged ORF Clone
CAT#: RC200538
- TrueORF®
PPP4C (Myc-DDK-tagged)-Human protein phosphatase 4, catalytic subunit (PPP4C)
ORF Plasmid: tGFP
"NM_002720" in other vectors (7)
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CNY 4,465.00
Cited in 1 publication. |
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | PP-X; PP4; PP4C; PPH3; PPP4; PPX |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Chromatograms |
CHROMATOGRAMS
![]() Sequencher program is needed, download here. |
Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
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ACCN | NM_002720 |
ORF Size | 921 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_002720.3 |
RefSeq Size | 1429 bp |
RefSeq ORF | 924 bp |
Locus ID | 5531 |
UniProt ID | P60510 |
Domains | Metallophos, PP2Ac |
Protein Families | Druggable Genome, Phosphatase |
MW | 35.1 kDa |
Gene Summary | Protein phosphatase that is involved in many processes such as microtubule organization at centrosomes, maturation of spliceosomal snRNPs, apoptosis, DNA repair, tumor necrosis factor (TNF)-alpha signaling, activation of c-Jun N-terminal kinase MAPK8, regulation of histone acetylation, DNA damage checkpoint signaling, NF-kappa-B activation and cell migration. The PPP4C-PPP4R1 PP4 complex may play a role in dephosphorylation and regulation of HDAC3. The PPP4C-PPP4R2-PPP4R3A PP4 complex specifically dephosphorylates H2AFX phosphorylated on Ser-140 (gamma-H2AFX) generated during DNA replication and required for DNA double strand break repair. Dephosphorylates NDEL1 at CDK1 phosphorylation sites and negatively regulates CDK1 activity in interphase (By similarity). In response to DNA damage, catalyzes RPA2 dephosphorylation, an essential step for DNA repair since it allows the efficient RPA2-mediated recruitment of RAD51 to chromatin.[UniProtKB/Swiss-Prot Function] |
Citations (1)
The use of this cDNA Clones has been cited in the following citations: |
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PATZ1 induces PP4R2 to form a negative feedback loop on IKK/NF-κB signaling in lung cancer
,Ho, MY;Liang, CM;Liang, SM;,
Oncotarget
,PubMed ID 27391343
[PPP4C]
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Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC200538L1 | Lenti ORF clone of Human protein phosphatase 4, catalytic subunit (PPP4C), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200538L2 | Lenti ORF clone of Human protein phosphatase 4, catalytic subunit (PPP4C), mGFP tagged |
CNY 5,890.00 |
|
RC200538L3 | Lenti ORF clone of Human protein phosphatase 4, catalytic subunit (PPP4C), Myc-DDK-tagged |
CNY 4,800.00 |
|
RC200538L4 | Lenti ORF clone of Human protein phosphatase 4, catalytic subunit (PPP4C), mGFP tagged |
CNY 4,800.00 |
|
RG200538 | PPP4C (tGFP-tagged) - Human protein phosphatase 4, catalytic subunit (PPP4C) |
CNY 4,000.00 |
|
SC118440 | PPP4C (untagged)-Human protein phosphatase 4, catalytic subunit (PPP4C) |
CNY 2,400.00 |
|
SC320616 | PPP4C (untagged)-Human protein phosphatase 4, catalytic subunit (PPP4C) |
CNY 2,400.00 |