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HJURP (NM_018410) Human Tagged ORF Clone
CAT＃: RC201283
HJURP (Myc-DDK-tagged)-Human Holliday junction recognition protein (HJURP)
ORF Plasmid: tGFP
"NM_018410" in other vectors (7)
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CNY 5,480.00
CNY 7,890.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | FAKTS; hFLEG1; URLC9 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC201283 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCTGGGTACGCTGCGCGCCATGGAGGGCGAGGACGTGGAAGACGACCAGCTGCTGCAGAAGCTCAGGG CCAGTCGCCGCCGCTTCCAGAGGCGCATGCAGCGGCTGATAGAGAAGTACAACCAGCCCTTCGAGGACAC CCCGGTGGTGCAAATGGCCACGCTGACCTACGAGACGCCACAGGGATTGAGAATTTGGGGTGGAAGACTA ATAAAGGAAAGAAACAAAGGAGAGATCCAGGACTCCTCCATGAAGCCCGCGGACAGGACAGATGGCTCCG TGCAAGCTGCAGCCTGGGGTCCTGAGCTTCCCTCGCACCGCACAGTCCTGGGAGCCGATTCAAAAAGCGG TGAGGTCGATGCCACGTCAGACCAGGAAGAGTCAGTTGCTTGGGCCTTAGCACCTGCAGTGCCTCAAAGC CCTTTGAAAAATGAATTAAGAAGGAAATACTTGACCCAAGTGGATATACTGCTACAAGGTGCAGAGTATT TTGAGTGTGCAGGTAACAGAGCTGGAAGGGATGTACGTGTGACTCCGCTGCCTTCACTGGCCTCACCTGC CGTGCCTGCCCCCGGATACTGCAGTCGTATCTCCGGAAAGAGTCCTGGTGACCCAGCGAAACCAGCTTCA TCTCCCAGAGAATGGGATCCTTTGCATCCTTCCTCCACAGACATGGCCTTAGTACCTAGAAATGACAGCC TCTCCCTACAAGAGACCAGTAGCAGCAGCTTCTTAAGCAGCCAGCCCTTTGAAGATGATGACATTTGCAA TGTGACCATCAGTGACCTGTACGCAGGGATGCTGCACTCCATGAGCCGGCTGTTGAGCACAAAGCCATCA AGCATCATCTCCACCAAAACGTTCATCATGCAAAACTGGAACTGCAGGAGGAGGCACAGATATAAGAGCA GGATGAACAAAACATATTGCAAAGGAGCCAGACGTTCTCAGAGGAGCTCCAAGGAGAACTTCATACCCTG CTCTGAGCCTGTGAAAGGGACAGGGGCATTAAGAGATTGCAAGAACGTATTAGATGTTTCTTGCCGTAAG ACAGGTTTAAAATTGGAAAAAGCTTTTCTTGAAGTCAACAGACCCCAAATCCATAAGTTAGATCCAAGTT GGAAGGAGCGCAAAGTGACACCCTCGAAGTATTCTTCCTTGATTTACTTCGACTCCAGTGCAACATATAA TCTTGATGAGGAAAATAGATTTAGGACATTAAAATGGTTAATTTCTCCTGTAAAAATAGTTTCCAGACCA ACAATACGACAGGGCCATGGAGAGAACCGTCAGAGGGAGATTGAAATCCGATTTGATCAGCTTCATCGGG AATATTGCCTGAGTCCCAGGAACCAGCCTCGCCGGATGTGCCTCCCGGACTCCTGGGCCATGAACATGTA CAGAGGGGGTCCTGCGAGTCCTGGTGGCCTTCAGGGCTTAGAAACCCGCAGGCTGAGTTTACCTTCCAGC AAAGCAAAAGCAAAAAGTTTAAGTGAGGCTTTTGAAAACCTAGGCAAAAGATCTCTGGAAGCAGGTAGGT GCCTGCCCAAGAGCGATTCATCTTCATCACTTCCAAAGACCAACCCCACACACAGCGCAACTCGCCCGCA GCAGACATCTGACCTTCACGTTCAGGGAAATAGTTCTGGAATATTTAGAAAGTCAGTGTCACCCAGCAAA ACTCTTTCAGTCCCAGATAAAGAAGTGCCAGGCCACGGAAGGAATCGTTACGATGAAATTAAAGAAGAAT TTGACAAGCTTCATCAAAAGTATTGCCTCAAATCTCCTGGGCAGATGACAGTGCCTTTATGTATTGGAGT GTCTACAGATAAAGCAAGTATGGAAGTTCGATATCAAACAGAAGGCTTCTTAGGAAAATTAAATCCAGAC CCTCACTTCCAGGGTTTCCAGAAGTTGCCATCATCACCCCTGGGGTGCAGAAAAAGTCTACTGGGCTCAA CTGCAATTGAGGCTCCTTCATCTACATGTGTTGCTCGTGCCATCACGAGGGATGGCACGAGGGACCATCA GTTCCCTGCAAAAAGACCCAGGCTATCAGAACCCCAGGGCTCCGGACGCCAGGGCAATTCCCTGGGTGCC TCAGATGGGGTGGACAACACCGTCAGACCGGGAGACCAGGGCAGCTCTTCACAGCCCAACTCAGAAGAGA GAGGAGAGAACACGTCTTACAGGATGGAAGAGAAAAGTGATTTCATGCTAGAAAAATTGGAAACTAAAAG TGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC201283 protein sequence
Red=Cloning site Green=Tags(s) MLGTLRAMEGEDVEDDQLLQKLRASRRRFQRRMQRLIEKYNQPFEDTPVVQMATLTYETPQGLRIWGGRL IKERNKGEIQDSSMKPADRTDGSVQAAAWGPELPSHRTVLGADSKSGEVDATSDQEESVAWALAPAVPQS PLKNELRRKYLTQVDILLQGAEYFECAGNRAGRDVRVTPLPSLASPAVPAPGYCSRISGKSPGDPAKPAS SPREWDPLHPSSTDMALVPRNDSLSLQETSSSSFLSSQPFEDDDICNVTISDLYAGMLHSMSRLLSTKPS SIISTKTFIMQNWNCRRRHRYKSRMNKTYCKGARRSQRSSKENFIPCSEPVKGTGALRDCKNVLDVSCRK TGLKLEKAFLEVNRPQIHKLDPSWKERKVTPSKYSSLIYFDSSATYNLDEENRFRTLKWLISPVKIVSRP TIRQGHGENRQREIEIRFDQLHREYCLSPRNQPRRMCLPDSWAMNMYRGGPASPGGLQGLETRRLSLPSS KAKAKSLSEAFENLGKRSLEAGRCLPKSDSSSSLPKTNPTHSATRPQQTSDLHVQGNSSGIFRKSVSPSK TLSVPDKEVPGHGRNRYDEIKEEFDKLHQKYCLKSPGQMTVPLCIGVSTDKASMEVRYQTEGFLGKLNPD PHFQGFQKLPSSPLGCRKSLLGSTAIEAPSSTCVARAITRDGTRDHQFPAKRPRLSEPQGSGRQGNSLGA SDGVDNTVRPGDQGSSSQPNSEERGENTSYRMEEKSDFMLEKLETKSV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_018410 |
| ORF Size | 2244 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_018410.5 |
| RefSeq Size | 3189 bp |
| RefSeq ORF | 2247 bp |
| Locus ID | 55355 |
| UniProt ID | Q8NCD3 |
| Protein Families | Druggable Genome |
| MW | 83.5 kDa |
| Gene Summary | Centromeric protein that plays a central role in the incorporation and maintenance of histone H3-like variant CENPA at centromeres. Acts as a specific chaperone for CENPA and is required for the incorporation of newly synthesized CENPA molecules into nucleosomes at replicated centromeres. Prevents CENPA-H4 tetramerization and prevents premature DNA binding by the CENPA-H4 tetramer. Directly binds Holliday junctions.[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC201283L1 | Lenti ORF clone of Human Holliday junction recognition protein (HJURP), Myc-DDK-tagged |
CNY 7,880.00 |
|
| RC201283L2 | Lenti ORF clone of Human Holliday junction recognition protein (HJURP), mGFP tagged |
CNY 9,790.00 |
|
| RC201283L3 | Lenti ORF clone of Human Holliday junction recognition protein (HJURP), Myc-DDK-tagged |
CNY 9,790.00 |
|
| RC201283L4 | Lenti ORF clone of Human Holliday junction recognition protein (HJURP), mGFP tagged |
CNY 9,790.00 |
|
| RG201283 | HJURP (tGFP-tagged) - Human Holliday junction recognition protein (HJURP) |
CNY 8,650.00 |
|
| SC318229 | HJURP (untagged)-Human Holliday junction recognition protein (HJURP) |
CNY 14,350.00 |
|
| SC320017 | HJURP (untagged)-Human Holliday junction recognition protein (HJURP) |
CNY 2,950.00 |
