NHP2 (NM_017838) Human Tagged ORF Clone
CAT#: RC203033
NHP2 (Myc-DDK-tagged)-Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1
ORF Plasmid: tGFP
"NM_017838" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 1,200.00
CNY 3,705.00
CNY 300.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | Myc-DDK |
Synonyms | DKCB2; NHP2P; NOLA2 |
Vector | pCMV6-Entry |
E. coli Selection | Kanamycin (25 ug/mL) |
Mammalian Cell Selection | Neomycin |
Sequence Data |
>RC203033 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCAAAATAAAGGCAGATCCCGACGGGCCCGAGGCTCAGGCGGAGGCGTGTTCCGGGGAGCGCACCT ACCAGGAGCTGCTGGTCAACCAGAACCCCATCGCGCAGCCCCTGGCTTCTCGCCGCCTCACGCGGAAGCT CTACAAATGCATCAAGAAAGCGGTGAAGCAGAAGCAGATTCGGCGCGGGGTGAAAGAGGTTCAGAAATTT GTCAACAAAGGAGAAAAAGGGATCATGGTTTTGGCAGGAGACACACTGCCCATTGAGGTATACTGCCATC TCCCAGTCATGTGTGAGGACCGAAATTTGCCCTATGTCTATATCCCCTCTAAGACGGACCTGGGTGCAGC CGCAGGCTCCAAGCGCCCCACCTGTGTGATAATGGTCAAGCCCCATGAGGAGTACCAGGAGGCTTACGAT GAGTGCCTGGAGGAGGTGCAGTCCCTGCCCCTACCCCTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203033 protein sequence
Red=Cloning site Green=Tags(s) MTKIKADPDGPEAQAEACSGERTYQELLVNQNPIAQPLASRRLTRKLYKCIKKAVKQKQIRRGVKEVQKF VNKGEKGIMVLAGDTLPIEVYCHLPVMCEDRNLPYVYIPSKTDLGAAAGSKRPTCVIMVKPHEEYQEAYD ECLEEVQSLPLPL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
Chromatograms |
CHROMATOGRAMS
Sequencher program is needed, download here. |
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_017838 |
ORF Size | 459 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_017838.4 |
RefSeq Size | 867 bp |
RefSeq ORF | 462 bp |
Locus ID | 55651 |
UniProt ID | Q9NX24 |
Domains | Ribosomal_L7Ae |
MW | 17.2 kDa |
Gene Summary | This gene is a member of the H/ACA snoRNPs (small nucleolar ribonucleoproteins) gene family. snoRNPs are involved in various aspects of rRNA processing and modification and have been classified into two families: C/D and H/ACA. The H/ACA snoRNPs also include the DKC1, NOLA1 and NOLA3 proteins. These four H/ACA snoRNP proteins localize to the dense fibrillar components of nucleoli and to coiled (Cajal) bodies in the nucleus. Both 18S rRNA production and rRNA pseudouridylation are impaired if any one of the four proteins is depleted. The four H/ACA snoRNP proteins are also components of the telomerase complex. This gene encodes a protein related to Saccharomyces cerevisiae Nhp2p. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Oct 2008] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC203033L3 | Lenti ORF clone of Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
RC203033L4 | Lenti ORF clone of Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
RG203033 | NHP2 (tGFP-tagged) - Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1 |
CNY 2,800.00 |
|
SC111338 | NHP2 (untagged)-Human NHP2 ribonucleoprotein homolog (yeast) (NHP2), transcript variant 1 |
CNY 1,200.00 |