United States
Germany
Japan
United Kingdom
China
POLG2 (NM_007215) Human Tagged ORF Clone
CAT＃: RC203462
POLG2 (Myc-DDK-tagged)-Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein
ORF Plasmid: tGFP
"NM_007215" in other vectors (6)
Need custom modification / cloning service?
Get a free quote
CNY 3,656.00
CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | HP55; MTDPS16; MTPOLB; PEOA4; POLB; POLG-BETA; POLGB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203462 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCGCTCTCGTGTAGCCGTCAGGGCCTGCCATAAGGTCTGCAGGTGCCTGTTGTCTGGGTTTGGGGGTC GAGTAGATGCGGGGCAGCCGGAGCTGTTGACGGAAAGGAGTAGCCCCAAAGGAGGGCATGTGAAGTCGCA CGCGGAGCTCGAGGGGAACGGCGAGCACCCAGAAGCCCCCGGGTCTGGAGAGGGAAGCGAGGCGCTGTTA GAGATCTGTCAGAGAAGGCATTTCCTAAGTGGAAGCAAGCAGCAGCTTAGCCGGGATTCTCTTCTGAGTG GGTGCCACCCCGGCTTCGGACCCTTGGGCGTAGAGTTGCGGAAGAACCTGGCCGCAGAATGGTGGACCTC GGTGGTGGTGTTCAGGGAGCAGGTATTCCCGGTGGACGCCCTCCACCACAAACCAGGCCCTTTGCTACCC GGGGACAGTGCCTTCAGGTTAGTTTCTGCAGAAACTCTACGCGAAATCTTGCAAGACAAAGAGCTGAGTA AGGAACAGCTAGTAGCATTTCTTGAGAACGTATTAAAAACTTCTGGGAAACTACGGGAGAACCTTCTTCA CGGTGCCTTGGAACACTATGTTAATTGCCTGGATCTGGTAAACAAGAGGCTACCTTATGGCCTTGCTCAG ATTGGAGTGTGTTTTCATCCTGTTTTTGACACTAAGCAGATACGAAATGGTGTTAAAAGTATTGGTGAGA AGACTGAAGCTTCGTTAGTATGGTTTACTCCTCCGAGAACTTCAAACCAGTGGCTTGATTTCTGGTTACG TCATCGACTCCAGTGGTGGAGAAAGTTTGCCATGAGTCCATCTAACTTCAGCAGCAGTGACTGTCAGGAT GAAGAAGGCCGGAAAGGAAACAAACTTTACTACAATTTTCCCTGGGGAAAGGAGTTAATAGAAACCCTGT GGAACCTAGGAGATCACGAACTTTTACACATGTATCCTGGCAATGTGTCTAAATTACATGGCCGAGATGG ACGAAAAAATGTGGTTCCTTGTGTTCTCTCTGTAAATGGGGACCTAGACCGAGGCATGCTGGCCTACCTC TATGATTCTTTCCAGCTGACAGAGAACTCCTTTACAAGAAAGAAAAATCTTCATAGAAAGGTACTTAAAC TTCACCCTTGTTTAGCCCCTATTAAGGTTGCTTTGGATGTAGGAAGAGGCCCCACATTGGAACTAAGACA GGTTTGTCAAGGGCTATTTAATGAGTTACTAGAAAATGGGATTTCTGTGTGGCCTGCTTATTTGGAAACT ATGCAGTCCTCATTGGAACAACTTTATTCGAAGTATGATGAAATGAGTATTCTCTTCACAGTTTTGGTTA CTGAAACTACTTTGGAGAATGGATTAATACATCTGAGAAGCAGAGACACCACAATGAAGGAAATGATGCA TATATCCAAATTAAAAGACTTTTTGATTAAGTATATATCATCAGCTAAGAATGTA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203462 protein sequence
Red=Cloning site Green=Tags(s) MRSRVAVRACHKVCRCLLSGFGGRVDAGQPELLTERSSPKGGHVKSHAELEGNGEHPEAPGSGEGSEALL EICQRRHFLSGSKQQLSRDSLLSGCHPGFGPLGVELRKNLAAEWWTSVVVFREQVFPVDALHHKPGPLLP GDSAFRLVSAETLREILQDKELSKEQLVAFLENVLKTSGKLRENLLHGALEHYVNCLDLVNKRLPYGLAQ IGVCFHPVFDTKQIRNGVKSIGEKTEASLVWFTPPRTSNQWLDFWLRHRLQWWRKFAMSPSNFSSSDCQD EEGRKGNKLYYNFPWGKELIETLWNLGDHELLHMYPGNVSKLHGRDGRKNVVPCVLSVNGDLDRGMLAYL YDSFQLTENSFTRKKNLHRKVLKLHPCLAPIKVALDVGRGPTLELRQVCQGLFNELLENGISVWPAYLET MQSSLEQLYSKYDEMSILFTVLVTETTLENGLIHLRSRDTTMKEMMHISKLKDFLIKYISSAKNV TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_007215 |
| ORF Size | 1455 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_007215.4 |
| RefSeq Size | 1607 bp |
| RefSeq ORF | 1458 bp |
| Locus ID | 11232 |
| UniProt ID | Q9UHN1 |
| Domains | HGTP_anticodon |
| Protein Families | Stem cell - Pluripotency |
| Protein Pathways | Metabolic pathways |
| MW | 54.9 kDa |
| Gene Summary | This gene encodes the processivity subunit of the mitochondrial DNA polymerase gamma. The encoded protein forms a heterotrimer containing one catalytic subunit and two processivity subunits. This protein enhances DNA binding and promotes processive DNA synthesis. Mutations in this gene result in autosomal dominant progressive external ophthalmoplegia with mitochondrial DNA deletions.[provided by RefSeq, Sep 2009] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203462L1 | Lenti ORF clone of Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203462L2 | Lenti ORF clone of Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RC203462L3 | Lenti ORF clone of Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC203462L4 | Lenti ORF clone of Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein, mGFP tagged |
CNY 5,890.00 |
|
| RG203462 | POLG2 (tGFP-tagged) - Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein |
CNY 4,370.00 |
|
| SC115638 | POLG2 (untagged)-Human polymerase (DNA directed), gamma 2, accessory subunit (POLG2), nuclear gene encoding mitochondrial protein |
CNY 3,656.00 |
