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TM9SF4 (NM_014742) Human Tagged ORF Clone
CAT＃: RC203648
TM9SF4 (Myc-DDK-tagged)-Human transmembrane 9 superfamily protein member 4 (TM9SF4)
ORF Plasmid: tGFP
"NM_014742" in other vectors (7)
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CNY 4,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | dJ836N17.2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC203648 representing NM_014742
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGTGTGAAACAAGCGCCTTCTATGTGCCTGGGGTCGCGCCTATCAACTTCCACCAGAACGATCCCGTAG AAATCAAGGCTGTGAAGCTCACCAGCTCTCGAACCCAGCTACCTTATGAATACTATTCACTGCCCTTCTG CCAGCCCAGCAAGATAACCTACAAGGCAGAGAATCTGGGAGAGGTGCTGAGAGGGGACCGGATTGTCAAC ACCCCTTTCCAGGTTCTCATGAACAGCGAGAAGAAGTGTGAAGTTCTGTGCAGCCAGTCCAACAAGCCAG TGACCCTGACAGTGGAGCAGAGCCGACTCGTGGCCGAGCGGATCACAGAAGACTACTACGTCCACCTCAT TGCTGACAACCTGCCTGTGGCCACCCGGCTGGAGCTCTACTCCAACCGAGACAGCGATGACAAGAAGAAG GAAAAAGATGTGCAGTTTGAACACGGCTACCGGCTCGGCTTCACAGATGTCAACAAGATCTACCTGCACA ACCACCTCTCATTCATCCTTTACTATCATCGGGAGGACATGGAAGAGGACCAGGAGCACACGTACCGTGT CGTCCGCTTCGAGGTGATTCCCCAGAGCATCAGGCTGGAGGACCTCAAAGCAGATGAGAAGAGTTCGTGC ACTCTGCCTGAGGGTACCAACTCCTCGCCCCAAGAAATTGACCCCACCAAGGAGAATCAGCTGTACTTCA CCTACTCTGTCCACTGGGAGGAAAGTGATATCAAATGGGCCTCTCGCTGGGACACTTACCTGACCATGAG TGACGTCCAGATCCACTGGTTTTCTATCATTAACTCCGTTGTTGTGGTCTTCTTCCTGTCAGGTATCCTG AGCATGATTATCATTCGGACCCTCCGGAAGGACATTGCCAACTACAACAAGGAGGATGACATTGAAGACA CCATGGAGGAGTCTGGGTGGAAGTTGGTGCACGGCGACGTCTTCAGGCCCCCCCAGTACCCCATGATCCT CAGCTCCCTGCTGGGCTCAGGCATTCAGCTGTTCTGTATGATCCTCATCGTCATCTTTGTAGCCATGCTT GGGATGCTGTCGCCCTCCAGCCGGGGAGCTCTCATGACCACAGCCTGCTTCCTCTTCATGTTCATGGGGG TGTTTGGCGGATTTTCTGCTGGCCGTCTGTACCGCACTTTAAAAGGCCATCGGTGGAAGAAAGGAGCCTT CTGTACGGCAACTCTGTACCCTGGTGTGGTTTTTGGCATCTGCTTCGTATTGAATTGCTTCATTTGGGGA AAGCACTCATCAGGAGCGGTGCCCTTTCCCACCATGGTGGCTCTGCTGTGCATGTGGTTCGGGATCTCCC TGCCCCTCGTCTACTTGGGCTACTACTTCGGCTTCCGAAAGCAGCCATATGACAACCCTGTGCGCACCAA CCAGATTCCCCGGCAGATCCCCGAGCAGCGGTGGTACATGAACCGATTTGTGGGCATCCTCATGGCTGGG ATCTTGCCCTTCGGCGCCATGTTCATCGAGCTCTTCTTCATCTTCAGTGCTATCTGGGAGAATCAGTTCT ATTACCTCTTTGGCTTCCTGTTCCTTGTTTTCATCATCCTGGTGGTATCCTGTTCACAAATCAGCATCGT CATGGTGTACTTCCAGCTGTGTGCAGAGGATTACCGCTGGTGGTGGAGAAATTTCCTAGTCTCCGGGGGC TCTGCATTCTACGTCCTGGTTTATGCCATCTTTTATTTCGTTAACAAGCTGGACATCGTGGAGTTCATCC CCTCTCTCCTCTACTTTGGCTACACGGCCCTCATGGTCTTGTCCTTCTGGCTGCTAACGGGTACCATCGG CTTCTATGCAGCCTACATGTTTGTTCGCAAGATCTATGCTGCTGTGAAGATAGAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC203648 representing NM_014742
Red=Cloning site Green=Tags(s) MCETSAFYVPGVAPINFHQNDPVEIKAVKLTSSRTQLPYEYYSLPFCQPSKITYKAENLGEVLRGDRIVN TPFQVLMNSEKKCEVLCSQSNKPVTLTVEQSRLVAERITEDYYVHLIADNLPVATRLELYSNRDSDDKKK EKDVQFEHGYRLGFTDVNKIYLHNHLSFILYYHREDMEEDQEHTYRVVRFEVIPQSIRLEDLKADEKSSC TLPEGTNSSPQEIDPTKENQLYFTYSVHWEESDIKWASRWDTYLTMSDVQIHWFSIINSVVVVFFLSGIL SMIIIRTLRKDIANYNKEDDIEDTMEESGWKLVHGDVFRPPQYPMILSSLLGSGIQLFCMILIVIFVAML GMLSPSSRGALMTTACFLFMFMGVFGGFSAGRLYRTLKGHRWKKGAFCTATLYPGVVFGICFVLNCFIWG KHSSGAVPFPTMVALLCMWFGISLPLVYLGYYFGFRKQPYDNPVRTNQIPRQIPEQRWYMNRFVGILMAG ILPFGAMFIELFFIFSAIWENQFYYLFGFLFLVFIILVVSCSQISIVMVYFQLCAEDYRWWWRNFLVSGG SAFYVLVYAIFYFVNKLDIVEFIPSLLYFGYTALMVLSFWLLTGTIGFYAAYMFVRKIYAAVKID TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014742 |
| ORF Size | 1875 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014742.2, NP_055557.1 |
| RefSeq Size | 3996 bp |
| RefSeq ORF | 1929 bp |
| Locus ID | 9777 |
| UniProt ID | Q92544 |
| Domains | EMP70 |
| Protein Families | Transmembrane |
| MW | 72.54 kDa |
| Gene Summary | Associates with proteins harboring glycine-rich transmembrane domains and ensures their efficient localization to the cell surface (PubMed:25999474). Regulates the assembly and activity of V-ATPase in colon cancer cells via its interaction with V-type proton ATPase subunit H (ATP6V1H) and contributes to V-ATPase-mediated pH alterations in cancer cells which play an important role in drug resistance and invasiveness of colon cancer cells (PubMed:25659576). Plays an important role in an atypical phagocytic activity of metastatic melanoma cells called cannibalism and is involved in the pH regulation of the intracellular vesicles in tumor cells (PubMed:19893578).[UniProtKB/Swiss-Prot Function] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC203648L1 | Lenti ORF clone of Human transmembrane 9 superfamily protein member 4 (TM9SF4), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203648L2 | Lenti ORF clone of Human transmembrane 9 superfamily protein member 4 (TM9SF4), mGFP tagged |
CNY 6,460.00 |
|
| RC203648L3 | Lenti ORF clone of Human transmembrane 9 superfamily protein member 4 (TM9SF4), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC203648L4 | Lenti ORF clone of Human transmembrane 9 superfamily protein member 4 (TM9SF4), mGFP tagged |
CNY 6,460.00 |
|
| RG203648 | TM9SF4 (tGFP-tagged) - Human transmembrane 9 superfamily protein member 4 (TM9SF4) |
CNY 5,040.00 |
|
| SC318098 | TM9SF4 (untagged)-Human transmembrane 9 superfamily protein member 4 (TM9SF4) |
CNY 7,220.00 |
|
| SC319560 | TM9SF4 (untagged)-Human transmembrane 9 superfamily protein member 4 (TM9SF4) |
CNY 4,664.00 |
