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UBC3B (UBE2R2) (NM_017811) Human Tagged ORF Clone
CAT＃: RC207977
UBE2R2 (Myc-DDK-tagged)-Human ubiquitin-conjugating enzyme E2R 2 (UBE2R2)
ORF Plasmid: tGFP
"NM_017811" in other vectors (4)
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CNY 2,400.00
CNY 3,705.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CDC34B; E2-CDC34B; UBC3B |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC207977 representing NM_017811
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGCCCAGCAGCAGATGACCAGCTCGCAGAAGGCCCTGATGCTCGAGCTGAAATCCCTGCAGGAGGAAC CGGTGGAGGGCTTCCGGATCACCCTGGTGGACGAGTCCGACCTCTACAACTGGGAGGTGGCCATCTTCGG ACCCCCCAACACCCTCTACGAAGGCGGCTACTTCAAGGCGCATATTAAATTTCCTATTGACTACCCCTAT TCACCACCTACCTTCAGATTCTTGACCAAAATGTGGCACCCCAACATTTATGAGAATGGAGATGTATGCA TTTCGATTCTTCATCCGCCTGTAGATGACCCACAGAGTGGAGAACTGCCTTCTGAAAGGTGGAATCCTAC TCAGAATGTGAGGACTATCCTATTAAGTGTAATCTCACTGCTTAATGAGCCCAACACCTTCTCCCCAGCC AATGTCGATGCTTCAGTTATGTTCAGGAAATGGAGAGACAGTAAAGGAAAAGACAAAGAATATGCTGAAA TTATTAGGAAACAAGTTTCAGCCACTAAGGCCGAAGCAGAAAAGGATGGAGTGAAGGTCCCCACAACCCT GGCGGAATACTGCATCAAAACTAAAGTGCCTTCCAATGACAACAGCTCAGATTTGCTTTACGACGACTTG TATGATGACGACATTGATGATGAAGATGAGGAGGAGGAAGATGCCGACTGTTATGATGATGATGATTCTG GGAATGAGGAGTCG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC207977 representing NM_017811
Red=Cloning site Green=Tags(s) MAQQQMTSSQKALMLELKSLQEEPVEGFRITLVDESDLYNWEVAIFGPPNTLYEGGYFKAHIKFPIDYPY SPPTFRFLTKMWHPNIYENGDVCISILHPPVDDPQSGELPSERWNPTQNVRTILLSVISLLNEPNTFSPA NVDASVMFRKWRDSKGKDKEYAEIIRKQVSATKAEAEKDGVKVPTTLAEYCIKTKVPSNDNSSDLLYDDL YDDDIDDEDEEEEDADCYDDDDSGNEES TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_017811 |
| ORF Size | 714 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_017811.4 |
| RefSeq Size | 4457 bp |
| RefSeq ORF | 717 bp |
| Locus ID | 54926 |
| UniProt ID | Q712K3 |
| Domains | UBCc |
| Protein Pathways | Ubiquitin mediated proteolysis |
| MW | 27 kDa |
| Gene Summary | Protein kinase CK2 is a ubiquitous and pleiotropic Ser/Thr protein kinase involved in cell growth and transformation. This gene encodes a protein similar to the E2 ubiquitin conjugating enzyme UBC3/CDC34. Studies suggest that CK2-dependent phosphorylation of this ubiquitin-conjugating enzyme functions by regulating beta-TrCP substrate recognition and induces its interaction with beta-TrCP, enhancing beta-catenin degradation. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC207977L3 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2R 2 (UBE2R2), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC207977L4 | Lenti ORF clone of Human ubiquitin-conjugating enzyme E2R 2 (UBE2R2), mGFP tagged |
CNY 5,890.00 |
|
| RG207977 | UBE2R2 (tGFP-tagged) - Human ubiquitin-conjugating enzyme E2R 2 (UBE2R2) |
CNY 4,000.00 |
|
| SC113961 | UBE2R2 (untagged)-Human ubiquitin-conjugating enzyme E2R 2 (UBE2R2) |
CNY 2,400.00 |
