HM74 (HCAR3) (NM_006018) Human Tagged Lenti ORF Clone
CAT#: RC208523L4
- LentiORF®
Lenti-ORF clone of HCAR3 (mGFP-tagged)-Human G protein-coupled receptor 109B (GPR109B)
"NM_006018" in other vectors (4)
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CNY 5,890.00
Specifications
Product Data | |
Type | Human Tagged ORF Clone |
Tag | mGFP |
Synonyms | GPR109B; HCA3; HM74; Puma-g; PUMAG |
Vector | pLenti-C-mGFP-P2A-Puro |
E. coli Selection | Chloramphenicol (34 ug/mL) |
Mammalian Cell Selection | Puromycin |
Sequence Data |
The ORF insert of this clone is exactly the same as(RC208523).
|
Restriction Sites | SgfI-MluI Cloning Scheme for this gene Plasmid Map |
ACCN | NM_006018 |
ORF Size | 1161 bp |
OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
Reference Data | |
RefSeq | NM_006018.2 |
RefSeq Size | 2152 bp |
RefSeq ORF | 1164 bp |
Locus ID | 8843 |
UniProt ID | P49019 |
Domains | 7tm_1 |
Protein Families | Druggable Genome, GPCR, Transmembrane |
MW | 44.9 kDa |
Gene Summary | Receptor for 3-OH-octanoid acid mediates a negative feedback regulation of adipocyte lipolysis to counteract prolipolytic influences under conditions of physiological or pathological increases in beta-oxidation rates. Acts as a low affinity receptor for nicotinic acid. This pharmacological effect requires nicotinic acid doses that are much higher than those provided by a normal diet.[UniProtKB/Swiss-Prot Function] |
Documents
Product Manuals |
FAQs |
SDS |
Resources
Other Versions
SKU | Description | Size | Price |
---|---|---|---|
RC208523 | HCAR3 (Myc-DDK-tagged)-Human G protein-coupled receptor 109B (GPR109B) |
CNY 3,656.00 |
|
RC208523L3 | Lenti-ORF clone of HCAR3 (Myc-DDK-tagged)-Human G protein-coupled receptor 109B (GPR109B) |
CNY 5,890.00 |
|
RG208523 | HCAR3 (tGFP-tagged) - Human G protein-coupled receptor 109B (GPR109B) |
CNY 4,370.00 |
|
SC116358 | HCAR3 (untagged)-Human G protein-coupled receptor 109B (GPR109B) |
CNY 2,950.00 |