ZNF343 (NM_024325) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	dJ734P14.5
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC209416 ORF sequence, codon optimized. Due to the complexity of NM_024325, the ORF clone is codon optimized for mammalian Expression. The nucleotide sequence differs from the reference sequence, yet the amino acid sequence remains identical. Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTGCCTTATCCGTCTGCTTTGGGGGACCAGTACTGGGAAGAAATACTGTTGCCTAAGAATGGAG AGAATGTGGAGACAATGAAAAAACTCACGCAGAATCACAAGGCCAAAGGGCTGCCTTCCAATGATACAGA CTGTCCCCAGAAGAAAGAGGGGAAAGCGCAAATTGTGGTTCCTGTGACATTCCGCGATGTGACTGTGATC TTTACCGAGGCCGAATGGAAACGCCTGTCCCCCGAGCAGAGAAATCTGTATAAGGAGGTTATGCTGGAGA ACTATCGAAACCTCCTCTCCCTTGCCGAGCCAAAGCCTGAAATTTACACCTGCAGCTCATGTCTGTTGGC ATTCAGTTGTCAACAGTTCCTGAGTCAACATGTACTGCAAATCTTCCTGGGGCTCTGCGCCGAGAACCAT TTTCATCCCGGCAATAGCTCTCCTGGCCATTGGAAGCAACAAGGGCAACAGTATTCACATGTGTCTTGCT GGTTTGAAAATGCCGAGGGTCAAGAGCGCGGCGGGGGCTCTAAGCCTTGGTCCGCGAGAACTGAAGAAAG AGAAACCAGTCGGGCTTTCCCTAGTCCGCTCCAACGACAGAGCGCGAGTCCGAGAAAGGGAAACATGGTA GTGGAGACTGAACCCTCTTCAGCACAGCGGCCCAATCCCGTTCAGTTGGACAAAGGGCTTAAAGAGCTTG AGACGTTGAGGTTCGGCGCCATCAATTGCAGGGAGTATGAACCCGACCACAACTTGGAAAGCAATTTTAT TACCAACCCACGCACACTCCTGGGGAAAAAGCCATACATCTGCAGCGACTGCGGCAGATCCTTCAAAGAC CGCTCTACTCTGATCAGACACCACCGCATCCATTCAATGGAGAAGCCCTATGTGTGCAGCGAGTGCGGCC GGGGCTTCAGCCAGAAGTCTAACCTGAGCCGGCACCAAAGAACTCACTCAGAGGAGAAGCCGTATCTCTG TAGAGAATGTGGCCAGAGTTTTCGATCTAAGTCTATCCTTAACAGGCACCAGTGGACCCATTCCGAAGAA AAGCCTTACGTATGTTCCGAATGCGGGAGAGGGTTCTCAGAAAAAAGCTCCTTCATTAGACACCAGCGGA CGCATAGTGGTGAGAAGCCATACGTCTGTCTGGAGTGCGGGCGAAGTTTCTGCGATAAGTCTACCTTGCG CAAGCATCAGCGAATACACTCAGGCGAGAAGCCATACGTGTGTCGCGAGTGTGGCCGCGGCTTTAGCCAG AACTCAGACCTTATCAAACATCAGAGAACGCATCTGGACGAAAAACCATACGTCTGCCGCGAATGCGGAA GAGGCTTTTGTGATAAGAGTACCTTGATAATTCACGAAAGAACACACTCAGGGGAAAAGCCCTATGTGTG TGGCGAGTGCGGAAGGGGTTTCTCTCGAAAGAGCTTGTTGCTGGTGCACCAGAGGACCCATAGCGGAGAA AAGCATTACGTGTGCCGCGAGTGCAGAAGGGGATTTTCCCAGAAATCTAACCTGATCAGGCACCAGCGGA CCCACTCCAACGAAAAACCATACATCTGCCGGGAATGCGGTCGAGGATTCTGTGACAAGTCCACCCTGAT CGTCCATGAGAGGACACACTCAGGTGAAAAACCATACGTTTGTTCAGAGTGTGGAAGAGGATTTTCTAGA AAATCCCTGCTCCTTGTTCACCAGCGGACCCATTCAGGCGAGAAACACTATGTTTGCCGGGAGTGTGGGC GGGGATTCTCTCACAAAAGCAACCTTATTCGCCATCAGAGAACACAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC209416 representing NM_024325 Red=Cloning site Green=Tags(s) MMLPYPSALGDQYWEEILLPKNGENVETMKKLTQNHKAKGLPSNDTDCPQKKEGKAQIVVPVTFRDVTVI FTEAEWKRLSPEQRNLYKEVMLENYRNLLSLAEPKPEIYTCSSCLLAFSCQQFLSQHVLQIFLGLCAENH FHPGNSSPGHWKQQGQQYSHVSCWFENAEGQERGGGSKPWSARTEERETSRAFPSPLQRQSASPRKGNMV VETEPSSAQRPNPVQLDKGLKELETLRFGAINCREYEPDHNLESNFITNPRTLLGKKPYICSDCGRSFKD RSTLIRHHRIHSMEKPYVCSECGRGFSQKSNLSRHQRTHSEEKPYLCRECGQSFRSKSILNRHQWTHSEE KPYVCSECGRGFSEKSSFIRHQRTHSGEKPYVCLECGRSFCDKSTLRKHQRIHSGEKPYVCRECGRGFSQ NSDLIKHQRTHLDEKPYVCRECGRGFCDKSTLIIHERTHSGEKPYVCGECGRGFSRKSLLLVHQRTHSGE KHYVCRECRRGFSQKSNLIRHQRTHSNEKPYICRECGRGFCDKSTLIVHERTHSGEKPYVCSECGRGFSR KSLLLVHQRTHSGEKHYVCRECGRGFSHKSNLIRHQRTH TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_024325
ORF Size	1797 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_024325.3, NM_024325.4, NM_024325.5, NP_077301.4
RefSeq Size	3644 bp
RefSeq ORF	1800 bp
Locus ID	79175
UniProt ID	Q6P1L6
Domains	KRAB, zf-C2H2
Protein Families	Transcription Factors
MW	69.2 kDa
Gene Summary	May be involved in transcriptional regulation.[UniProtKB/Swiss-Prot Function]

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