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RAB11 FIP2 (RAB11FIP2) (NM_014904) Human Tagged ORF Clone
CAT＃: RC210414
RAB11FIP2 (Myc-DDK-tagged)-Human RAB11 family interacting protein 2 (class I) (RAB11FIP2)
ORF Plasmid: tGFP
"NM_014904" in other vectors (4)
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CNY 3,816.00
CNY 4,560.00
| Cited in 1 publication. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | nRip11; Rab11-FIP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210414 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGATGCTGTCCGAGCAAGCCCAAAAGTGGTTTCCAACCCACGTGCAGGTCACAGTGCTCCAAGCCAAAG ATCTGAAGCCAAAAGGCAAAAGTGGTACCAATGACACATACACTATAATTCAGCTGGGCAAGGAAAAGTA CTCCACCTCTGTAGCTGAGAAAACCCTTGAGCCAGTTTGGAAGGAGGAGGCCTCTTTCGAGCTACCTGGA TTGCTAATTCAGGGAAGTCCAGAGAAATACATTCTTTTCCTTATAGTTATGCACAGGTCCCTGGTGGGTC TGGATAAATTTTTAGGGCAGGTGGCAATCAATCTCAATGACATCTTTGAGGACAAACAAAGAAGGAAAAC AGAGTGGTTTAGATTAGAATCCAAACAAGGAAAACGAATCAAAAACAGGGGTGAGATAAAGGTCAATATT CAGTTTATGAGGAACAATATGACCGCAAGTATGTTTGACTTATCAATGAAGGACAAAACCAGATCTCCTT TTGCAAAGTTAAAAGATAAGATGAAGGGTAGAAAAAATGATGGAACATTTTCTGATACGTCTTCTGCAAT CATTCCAAGTACTCACATGCCCGATGCCAATAGTGAATTTTCAAGTGGTGAAATACAGATGAAATCCAAA CCAAAAAAGCCTTTTCTCTTGGGTCCTCAGCGACTCTCGTCAGCGCATTCAATGTCTGATTTATCTGGGT CCCATATGTCTTCTGAGAAACTGAAGGCTGGCACCATAGGTCAAACACATCTTCTCGGACACCAGTTAGA TTCCTTTGGAACAGTTCCAGAAAGTGGAAGTCTCAAATCTCCACACAGAAGAACATTAAGCTTTGATACT TCTAAAATGAACCAACCTGACAGCATTGTGGATGAAGGTGAATTGTGTTTCGGAAGACAAAATGACCCAT TTACAAATGTGACTGCTTCATTACCCCAAAAATTTGCAACACTGCCAAGGAAGAAAAATCCATTTGAAGA AAGCAGCGAAACATGGGACAGCAGCATGAATTTATTTTCAAAACCAATTGAAATAAGAAAAGAAAATAAA AGAGAGAAAAGGGAGAAAGTTAGCCTGTTTGAAAGAGTGACTGGAAAAAAAGATAGCAGAAGATCTGATA AACTTAACAATGGGGGATCTGATAGCCCTTGTGACTTGAAATCACCTAATGCATTTAGTGAAAATCGCCA GGACTATTTTGATTATGAGTCAACCAATCCATTTACAGCAAAATTCAGGGCTTCAAATATAATGCCATCT TCAAGTTTTCATATGAGTCCAACAAGCAATGAAGACCTCAGGAAAATCCCGGACAGCAACCCCTTTGATG CCACTGCAGGGTATCGTAGTCTGACCTATGAAGAGGTTCTACAGGAGCTGGTGAAACACAAAGAACTCCT TAGGAGGAAAGACACCCACATCCGGGAACTCGAGGACTACATCGACAACCTCCTTGTAAGGGTAATGGAA GAAACGCCCAGTATTCTCAGAGTGCCATATGAACCATCCAGGAAAGCTGGCAAATTCTCTAACAGT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210414 protein sequence
Red=Cloning site Green=Tags(s) MMLSEQAQKWFPTHVQVTVLQAKDLKPKGKSGTNDTYTIIQLGKEKYSTSVAEKTLEPVWKEEASFELPG LLIQGSPEKYILFLIVMHRSLVGLDKFLGQVAINLNDIFEDKQRRKTEWFRLESKQGKRIKNRGEIKVNI QFMRNNMTASMFDLSMKDKTRSPFAKLKDKMKGRKNDGTFSDTSSAIIPSTHMPDANSEFSSGEIQMKSK PKKPFLLGPQRLSSAHSMSDLSGSHMSSEKLKAGTIGQTHLLGHQLDSFGTVPESGSLKSPHRRTLSFDT SKMNQPDSIVDEGELCFGRQNDPFTNVTASLPQKFATLPRKKNPFEESSETWDSSMNLFSKPIEIRKENK REKREKVSLFERVTGKKDSRRSDKLNNGGSDSPCDLKSPNAFSENRQDYFDYESTNPFTAKFRASNIMPS SSFHMSPTSNEDLRKIPDSNPFDATAGYRSLTYEEVLQELVKHKELLRRKDTHIRELEDYIDNLLVRVME ETPSILRVPYEPSRKAGKFSNS TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_014904 |
| ORF Size | 1536 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_014904.1, NP_055719.1 |
| RefSeq Size | 6077 bp |
| RefSeq ORF | 1539 bp |
| Locus ID | 22841 |
| UniProt ID | Q7L804 |
| Domains | C2 |
| Protein Pathways | Endocytosis |
| MW | 58.3 kDa |
| Gene Summary | A Rab11 effector binding preferentially phosphatidylinositol 3,4,5-trisphosphate (PtdInsP3) and phosphatidic acid (PA) and acting in the regulation of the transport of vesicles from the endosomal recycling compartment (ERC) to the plasma membrane. Involved in insulin granule exocytosis. Also involved in receptor-mediated endocytosis and membrane trafficking of recycling endosomes, probably originating from clathrin-coated vesicles. Required in a complex with MYO5B and RAB11 for the transport of NPC1L1 to the plasma membrane. Also acts as a regulator of cell polarity. Plays an essential role in phagocytosis through a mechanism involving TICAM2, RAC1 and CDC42 Rho GTPases for controlling actin-dynamics.[UniProtKB/Swiss-Prot Function] |
Citations (1)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
FERARI is required for Rab11-dependent endocytic recycling.
,null,
Nature cell biology
,PubMed ID 31988382
[RAB11 FIP2]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210414L3 | Lenti ORF clone of Human RAB11 family interacting protein 2 (class I) (RAB11FIP2), Myc-DDK-tagged |
CNY 6,460.00 |
|
| RC210414L4 | Lenti ORF clone of Human RAB11 family interacting protein 2 (class I) (RAB11FIP2), mGFP tagged |
CNY 6,460.00 |
|
| RG210414 | RAB11FIP2 (tGFP-tagged) - Human RAB11 family interacting protein 2 (class I) (RAB11FIP2) |
CNY 5,040.00 |
|
| SC114749 | RAB11FIP2 (untagged)-Human RAB11 family interacting protein 2 (class I) (RAB11FIP2) |
CNY 3,824.00 |
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