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NOSTRIN (NM_001039724) Human Tagged ORF Clone
CAT＃: RC210661
NOSTRIN (Myc-DDK-tagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 2
ORF Plasmid: tGFP
"NM_001039724" in other vectors (4)
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CNY 3,768.00
CNY 4,470.00
Product images
CNY 300.00
CNY 1,999.00
CNY 2,700.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DaIP2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC210661 ORF sequence
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGACCCACTGACAGATTGTCCGTATAATAAAGTATACAAGAACCTAAAGGAGTTTTCTCAAAATG GAGAGAATTTCTGCAAACAGGTCACATCTGTTCTTCAGCAAAGGGCAAACCTGGAAATTAGCTATGCCAA AGGACTTCAGAAACTGGCAAGCAAGCTGAGCAAAGCATTACAGAACACGAGAAAAAGTTGTGTTAGCAGT GCCTGGGCCTGGGCCTCAGAGGGAATGAAATCCACAGCGGACCTGCATCAAAAACTTGGCAAAGCAATTG AATTGGAAGCAATAAAACCGACTTATCAAGTCCTAAATGTACAAGAGAAGAAGAGAAAATCACTTGACAA TGAAGTTGAAAAGACAGCAAATCTTGTCATTAGCAACTGGAATCAGCAAATTAAGGCCAAGAAGAAATTA ATGGTTAGTACCAAGAAACATGAAGCACTTTTCCAGCTTGTAGAAAGCTCCAAGCAATCTATGACTGAGA AGGAGAAGCGGAAGCTCCTCAATAAACTGACAAAATCAACTGAAAAGTTGGAAAAGGAAGATGAAAATTA CTACCAAAAAAACATGGCGGGTTATTCTACCAGACTGAAATGGGAAAACACACTAGAGAACTGCTACCAG AGCATTCTGGAGCTGGAGAAGGAAAGAATTCAACTTTTATGCAATAACTTAAACCAGTACAGCCAACATA TTTCTCTTTTTGGCCAAACCCTGACCACATGCCACACGCAGATTCACTGTGCCATCAGCAAGATTGACAT TGAAAAAGATATCCAGGCTGTAATGGAAGAAACTGCAATTTTATCTACAGAAAACAAATCTGAGTTCCTG TTAACGGATTACTTTGAAGAAGATCCTAACAGTGCAATGGATAAAGAGAGACGAAAGTCTTTACTAAAAC CAAAATTATTGAGACTGCAGAGAGACATTGAAAAAGCCTCAAAAGACAAGGAAGGCCTGGAACGAATGCT TAAAACGTACTCCAGCACCTCCTCCTTCTCTGATGCAAAGAGCCAGAAAGACACAGCAGCGTTAATGGAT GAGAACAATTTGAAACTAGACCTTTTGGAAGCGAACTCCTACAAACTGTCATCAATGTTAGCAGAACTTG AGCAAAGACCTCAACCCAGCCATCCTTGTAGTAATTCCATCTTCAGGTGGAGGGAAAAGGAGCATACTCA TAGCTATGTGAAAATATCTCGGCCTTTTTTAATGAAGAGATTAGAGAATATTGTGAGCAAGGCATCTTCT GGTGGGCAGAGCAATCCAGGTTCTTCAACTCCAGCCCCTGGTGCAGCCCAGCTCAGCAGCAGACTTTGCA AGGCCTTGTATTCTTTTCAAGCCAGGCAAGATGATGAGTTGAATTTGGAAAAGGGTGACATTGTGATTAT ACACGAGAAAAAAGAAGAAGGATGGTGGTTTGGATCTTTGAATGGGAAAAAAGGCCATTTTCCTGCCGCT TATGTGGAGGAGTTACCTTCAAATGCTGGCAACACAGCTACAAAGGCA ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC210661 protein sequence
Red=Cloning site Green=Tags(s) MRDPLTDCPYNKVYKNLKEFSQNGENFCKQVTSVLQQRANLEISYAKGLQKLASKLSKALQNTRKSCVSS AWAWASEGMKSTADLHQKLGKAIELEAIKPTYQVLNVQEKKRKSLDNEVEKTANLVISNWNQQIKAKKKL MVSTKKHEALFQLVESSKQSMTEKEKRKLLNKLTKSTEKLEKEDENYYQKNMAGYSTRLKWENTLENCYQ SILELEKERIQLLCNNLNQYSQHISLFGQTLTTCHTQIHCAISKIDIEKDIQAVMEETAILSTENKSEFL LTDYFEEDPNSAMDKERRKSLLKPKLLRLQRDIEKASKDKEGLERMLKTYSSTSSFSDAKSQKDTAALMD ENNLKLDLLEANSYKLSSMLAELEQRPQPSHPCSNSIFRWREKEHTHSYVKISRPFLMKRLENIVSKASS GGQSNPGSSTPAPGAAQLSSRLCKALYSFQARQDDELNLEKGDIVIIHEKKEEGWWFGSLNGKKGHFPAA YVEELPSNAGNTATKA TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001039724 |
| ORF Size | 1518 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001039724.2 |
| RefSeq Size | 1947 bp |
| RefSeq ORF | 1521 bp |
| Locus ID | 115677 |
| UniProt ID | Q8IVI9 |
| MW | 57.7 kDa |
| Gene Summary | Nitric oxide (NO) is a potent mediator in biologic processes such as neurotransmission, inflammatory response, and vascular homeostasis. NOSTRIN binds the enzyme responsible for NO production, endothelial NO synthase (ENOS; MIM 163729), and triggers the translocation of ENOS from the plasma membrane to vesicle-like subcellular structures, thereby attenuating ENOS-dependent NO production.[supplied by OMIM, Apr 2004] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC210661L3 | Lenti ORF clone of Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 2, Myc-DDK-tagged |
CNY 6,370.00 |
|
| RC210661L4 | Lenti ORF clone of Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 2, mGFP tagged |
CNY 6,370.00 |
|
| RG210661 | NOSTRIN (tGFP-tagged) - Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 2 |
CNY 4,940.00 |
|
| SC319846 | NOSTRIN (untagged)-Human nitric oxide synthase trafficker (NOSTRIN), transcript variant 2 |
CNY 3,776.00 |
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