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GPD2 (NM_001083112) Human Tagged ORF Clone
CAT＃: RC213341
- TrueORF®
GPD2 (Myc-DDK-tagged)-Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1
ORF Plasmid: tGFP
"NM_001083112" in other vectors (6)
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CNY 7,984.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GDH2; GPDM; mGDH; mGPDH |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC213341 representing NM_001083112.
Blue=ORF Red=Cloning site Green=Tag(s) GCTCGTTTAGTGAACCGTCAGAATTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTG GATCCGGTACCGAGGAGATCTGCCGCCGCGATCGCC ATGGCATTTCAAAAGGCAGTGAAAGGGACGATTCTTGTTGGAGGAGGTGCTCTTGCAACTGTTTTAGGA CTTTCTCAGTTTGCTCATTACAGAAGGAAACAAATGAACCTGGCCTATGTTAAAGCAGCAGACTGCATT TCAGAACCAGTTAACAGGGAGCCTCCTTCCAGAGAAGCTCAGCTACTGACTTTGCAAAACACATCTGAA TTTGATATCCTTGTTATTGGAGGAGGAGCAACAGGAAGTGGCTGTGCGCTAGATGCTGTCACCAGAGGA CTAAAAACAGCCCTTGTAGAAAGAGATGATTTCTCATCAGGGACCAGCAGCAGAAGCACTAAATTGATC CATGGTGGTGTGAGATATCTGCAGAAGGCCATCATGAAGTTGGATATTGAGCAGTATAGGATGGTAAAA GAAGCCCTTCATGAGCGTGCCAACCTGCTAGAAATTGCTCCCCATTTATCAGCTCCATTGCCTATAATG CTTCCAGTTTACAAGTGGTGGCAGTTACCTTACTACTGGGTAGGAATCAAGCTGTATGATTTGGTTGCA GGAAGCAATTGCCTAAAAAGCAGTTATGTCCTCAGCAAATCAAGAGCCCTTGAACATTTCCCAATGCTC CAGAAGGACAAACTGGTAGGAGCAATTGTCTACTATGACGGACAACATAACGATGCACGGATGAACCTT GCCATTGCTCTGACTGCTGCCAGGTATGGGGCTGCCACAGCCAATTACATGGAGGTAGTGAGCTTGCTC AAGAAGACAGACCCCCAGACAGGGAAAGTGCGTGTGAGCGGCGCACGGTGCAAGGATGTCCTCACAGGG CAGGAATTTGACGTGAGAGCCAAATGTGTTATCAATGCCACGGGACCTTTCACGGACTCTGTGCGCAAA ATGGATGATAAAGACGCAGCAGCTATCTGCCAGCCAAGTGCTGGTGTCCATATTGTGATGCCTGGTTAT TACAGCCCAGAGAGCATGGGACTTCTTGACCCAGCGACCAGTGATGGGCGAGTTATTTTCTTCTTACCC TGGCAAAAGATGACGATCGCTGGCACTACTGATACTCCAACTGATGTTACACACCATCCAATTCCTTCA GAAGAAGATATCAACTTCATTTTGAATGAAGTGCGTAATTACCTGAGTTGTGATGTTGAAGTGAGAAGA GGGGATGTCCTGGCAGCATGGAGTGGAATCCGTCCTCTTGTTACAGACCCCAAATCTGCAGATACTCAG TCTATCTCCCGAAATCATGTTGTTGATATCAGTGAGAGTGGCCTTATTACTATAGCAGGTGGAAAGTGG ACAACTTATCGGTCTATGGCAGAAGATACCATAAATGCTGCTGTCAAAACTCATAATTTAAAAGCAGGA CCAAGTAGAACAGTTGGGCTTTTCCTTCAAGGGGGTAAAGATTGGAGCCCCACACTCTACATTAGGCTT GTGCAGGATTATGGACTTGAAAGCGAGGTGGCACAGCATCTTGCCGCCACCTATGGTGATAAGGCCTTT GAGGTGGCCAAAATGGCAAGTGTGACTGGCAAAAGGTGGCCTATTGTTGGAGTACGTCTTGTGTCAGAA TTTCCATATATTGAAGCAGAGGTGAAATATGGGATTAAGGAGTATGCCTGCACTGCTGTGGATATGATT TCACGTCGTACTCGCCTGGCCTTTCTAAATGTCCAGGCAGCAGAGGAAGCCCTACCCAGGATTGTTGAA CTGATGGGCAGGGAACTGAATTGGGATGATTATAAGAAGCAGGAACAACTTGAAACAGCCAGGAAGTTT CTATATTATGAAATGGGCTATAAATCTCGATCAGAACAGTTAACAGATCGCTCTGAAATTAGCCTACTG CCTTCAGACATTGACAGGTATAAGAAGAGATTTCATAAGTTTGATGCAGACCAGAAAGGCTTTATTACC ATTGTTGATGTTCAGCGTGTATTAGAGAGTATCAATGTCCAAATGGATGAAAATACACTCCATGAAATT CTAAATGAAGTTGATTTGAATAAAAATGGACAGGTTGAACTCAATGAATTTTTGCAGCTGATGAGTGCT ATTCAAAAAGGAAGGGTATCTGGAAGCCGGCTTGCTATACTAATGAAAACTGCAGAAGAGAACCTCGAC AGAAGAGTTCCAATTCCAGTGGACCGTAGTTGTGGAGGATTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGAT TACAAGGATGACGACGATAAGGTTTAAACGGCCGGC >Peptide sequence encoded by RC213341
Blue=ORF Red=Cloning site Green=Tag(s) MAFQKAVKGTILVGGGALATVLGLSQFAHYRRKQMNLAYVKAADCISEPVNREPPSREAQLLTLQNTSE FDILVIGGGATGSGCALDAVTRGLKTALVERDDFSSGTSSRSTKLIHGGVRYLQKAIMKLDIEQYRMVK EALHERANLLEIAPHLSAPLPIMLPVYKWWQLPYYWVGIKLYDLVAGSNCLKSSYVLSKSRALEHFPML QKDKLVGAIVYYDGQHNDARMNLAIALTAARYGAATANYMEVVSLLKKTDPQTGKVRVSGARCKDVLTG QEFDVRAKCVINATGPFTDSVRKMDDKDAAAICQPSAGVHIVMPGYYSPESMGLLDPATSDGRVIFFLP WQKMTIAGTTDTPTDVTHHPIPSEEDINFILNEVRNYLSCDVEVRRGDVLAAWSGIRPLVTDPKSADTQ SISRNHVVDISESGLITIAGGKWTTYRSMAEDTINAAVKTHNLKAGPSRTVGLFLQGGKDWSPTLYIRL VQDYGLESEVAQHLAATYGDKAFEVAKMASVTGKRWPIVGVRLVSEFPYIEAEVKYGIKEYACTAVDMI SRRTRLAFLNVQAAEEALPRIVELMGRELNWDDYKKQEQLETARKFLYYEMGYKSRSEQLTDRSEISLL PSDIDRYKKRFHKFDADQKGFITIVDVQRVLESINVQMDENTLHEILNEVDLNKNGQVELNEFLQLMSA IQKGRVSGSRLAILMKTAEENLDRRVPIPVDRSCGGL TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001083112 |
| ORF Size | 2181 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001083112.3 |
| RefSeq Size | 6046 bp |
| RefSeq ORF | 2184 bp |
| Locus ID | 2820 |
| UniProt ID | P43304 |
| Protein Families | Druggable Genome, Transmembrane |
| Protein Pathways | Glycerophospholipid metabolism |
| MW | 80.9 kDa |
| Gene Summary | The protein encoded by this gene localizes to the inner mitochondrial membrane and catalyzes the conversion of glycerol-3-phosphate to dihydroxyacetone phosphate, using FAD as a cofactor. Along with GDP1, the encoded protein constitutes the glycerol phosphate shuttle, which reoxidizes NADH formed during glycolysis. Two transcript variants encoding the same protein have been found for this gene.[provided by RefSeq, Jan 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC213341L1 | Lenti ORF clone of Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 10,384.00 |
|
| RC213341L2 | Lenti ORF clone of Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,560.00 |
|
| RC213341L3 | Lenti ORF clone of Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1, Myc-DDK-tagged |
CNY 6,560.00 |
|
| RC213341L4 | Lenti ORF clone of Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1, mGFP tagged |
CNY 6,560.00 |
|
| RG213341 | GPD2 (tGFP-tagged) - Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 5,130.00 |
|
| SC316023 | GPD2 (untagged)-Human glycerol-3-phosphate dehydrogenase 2 (mitochondrial) (GPD2), nuclear gene encoding mitochondrial protein, transcript variant 1 |
CNY 13,970.00 |
