Cyclin G2 (CCNG2) (NM_004354) Human Tagged ORF Clone

CAT#: RC217068

CCNG2 (Myc-DDK-tagged)-Human cyclin G2 (CCNG2)

ORF Plasmid: DDK tGFP



  "NM_004354" in other vectors (6)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (5)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00


CCNG2 rabbit polyclonal antibody
    • 100 ul

CNY 1,999.00
CNY 3,280.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC217068 representing NM_004354
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGAAGGATTTGGGGGCAGAGCACTTGGCAGGTCATGAAGGGGTCCAACTTCTCGGGTTGTTGAACGTCT
ACCTGGAACAAGAAGAGAGATTCCAACCTCGAGAAAAAGGGCTGAGTTTGATTGAGGCTACCCCGGAGAA
TGATAACACTTTGTGTCCAGGATTGAGAAATGCCAAAGTTGAAGATTTAAGGAGTTTAGCCAACTTTTTT
GGATCTTGCACTGAAACTTTTGTCCTGGCTGTCAATATTTTGGACAGGTTCTTGGCTCTTATGAAGGTGA
AACCTAAACATTTGTCTTGCATTGGAGTCTGTTCTTTTTTGCTGGCTGCTAGAATAGTTGAAGAAGACTG
CAATATTCCATCCACTCATGATGTGATCCGGATTAGTCAGTGTAAATGTACTGCTTCTGACATAAAACGG
ATGGAAAAAATAATTTCAGAAAAATTGCACTATGAATTGGAAGCTACTACTGCCTTAAACTTTTTGCACT
TATACCATACTATTATACTTTGTCATACTTCAGAAAGGAAAGAAATACTGAGCCTTGATAAACTAGAAGC
TCAGCTGAAAGCTTGCAACTGCCGACTCATCTTTTCAAAAGCAAAACCATCTGTATTAGCCTTGTGCCTT
CTCAATTTGGAAGTGGAAACTTTGAAATCTGTTGAATTACTGGAAATTCTCTTGCTAGTTAAAAAACATT
CCAAGATTAATGACACTGAGTTCTTCTACTGGAGAGAGTTGGTTTCTAAATGCCTAGCCGAGTATTCTTC
TCCTGAATGTTGCAAACCAGATCTTAAGAAGTTGGTTTGGATCGTTTCAAGGCGCACAGCCCAGAACCTC
CACAACAGCTACTATAGTGTTCCTGAGCTGCCAACGATACCTGAGGGGGGTTGTTTTGATGAAAGTGAAA
GTGAGGACTCTTGTGAAGATATGAGTTGTGGAGAGGAGAGTCTCAGCAGCTCTCCTCCCAGTGATCAAGA
GTGCACCTTCTTTTTCAACTTCAAAGTGGCACAAACACTGTGCTTTCCATCT


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC217068 representing NM_004354
Red=Cloning site Green=Tags(s)

MKDLGAEHLAGHEGVQLLGLLNVYLEQEERFQPREKGLSLIEATPENDNTLCPGLRNAKVEDLRSLANFF
GSCTETFVLAVNILDRFLALMKVKPKHLSCIGVCSFLLAARIVEEDCNIPSTHDVIRISQCKCTASDIKR
MEKIISEKLHYELEATTALNFLHLYHTIILCHTSERKEILSLDKLEAQLKACNCRLIFSKAKPSVLALCL
LNLEVETLKSVELLEILLLVKKHSKINDTEFFYWRELVSKCLAEYSSPECCKPDLKKLVWIVSRRTAQNL
HNSYYSVPELPTIPEGGCFDESESEDSCEDMSCGEESLSSSPPSDQECTFFFNFKVAQTLCFPS

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_004354
ORF Size 1032 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_004354.3
RefSeq Size 2044 bp
RefSeq ORF 1035 bp
Locus ID 901
UniProt ID Q16589
Domains CYCLIN, cyclin
Protein Families Druggable Genome
Protein Pathways p53 signaling pathway
MW 38.7 kDa
Gene Summary The eukaryotic cell cycle is governed by cyclin-dependent protein kinases (CDKs) whose activities are regulated by cyclins and CDK inhibitors. The 8 species of cyclins reported in mammals, cyclins A through H, share a conserved amino acid sequence of about 90 residues called the cyclin box. The amino acid sequence of cyclin G is well conserved among mammals. The nucleotide sequence of cyclin G1 and cyclin G2 are 53% identical. Unlike cyclin G1, cyclin G2 contains a C-terminal PEST protein destabilization motif, suggesting that cyclin G2 expression is tightly regulated through the cell cycle. [provided by RefSeq, Jul 2008]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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