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myosin heavy chain 3 (MYH3) (NM_002470) Human Tagged ORF Clone
CAT＃: RC218098
- TrueORF®
MYH3 (Myc-DDK-tagged)-Human myosin, heavy chain 3, skeletal muscle, embryonic (MYH3)
ORF Plasmid: tGFP
"NM_002470" in other vectors (3)
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CNY 19,560.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CPSFS1A; CPSFS1B; CPSKF1A; CPSKF1B; DA2A; DA2B; DA2B3; DA8; HEMHC; MYHC-EMB; MYHSE1; SMHCE |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC218098 representing NM_002470
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGTAGTGACACTGAAATGGAAGTGTTCGGCATAGCTGCTCCTTTCCTCCGGAAGTCAGAAAAGGAGA GGATCGAGGCTCAGAACCAGCCCTTTGATGCCAAGACGTATTGCTTCGTGGTGGACTCAAAGGAAGAATA TGCCAAGGGGAAAATCAAGAGTTCTCAGGATGGGAAGGTCACTGTGGAAACTGAGGACAACAGGACCCTG GTGGTCAAACCAGAGGATGTGTACGCCATGAACCCCCCCAAGTTCGACAGGATCGAAGACATGGCCATGC TGACGCACCTGAATGAGCCAGCCGTGCTGTACAACCTGAAGGACCGTTACACATCTTGGATGATCTATAC CTACTCAGGCCTCTTCTGTGTCACTGTCAACCCCTACAAGTGGCTGCCGGTGTACAACCCCGAGGTGGTG GAAGGCTACCGAGGCAAAAAGCGCCAGGAGGCCCCACCCCACATCTTCTCCATCTCTGACAACGCCTATC AGTTCATGCTGACTGATCGTGAAAACCAGTCCATTCTGATCACCGGAGAATCCGGGGCAGGAAAGACTGT GAACACCAAACGGGTCATCCAGTACTTTGCAACAATTGCAGCTACTGGGGACCTGGCCAAGAAGAAGGAC TCCAAAATGAAGGGGACTCTGGAAGATCAAATCATCAGTGCCAATCCCCTGCTGGAGGCCTTTGGGAACG CCAAGACTGTGAGGAATGACAACTCCTCCCGTTTTGGCAAGTTCATCCGAATCCATTTTGGAACCACTGG GAAGCTGGCCTCTGCAGATATTGAAACTTATCTTCTGGAAAAATCAAGAGTCACTTTCCAGCTGAAGGCT GAAAGAAGCTACCACATCTTCTACCAGATTCTTTCTAACAAGAAGCCTGAGCTCATAGAGCTGCTGCTTA TTACGACCAACCCTTACGACTACCCGTTCATTAGCCAGGGGGAGATCCTGGTGGCCAGCATAGATGATGC AGAGGAGCTGCTGGCTACAGACAGCGCCATTGACATCCTGGGCTTCACCCCAGAAGAGAAATCTGGGCTC TACAAGCTGACGGGAGCCGTGATGCACTACGGGAACATGAAGTTCAAGCAGAAGCAGCGAGAGGAGCAGG CCGAGCCGGATGGCACAGAAGTGGCTGACAAAACAGCCTATCTGATGGGCCTGAACTCTTCGGACCTCCT AAAAGCTTTGTGCTTTCCTAGAGTGAAAGTTGGGAATGAGTACGTTACCAAAGGTCAAACTGTGGATCAG GTTCACCATGCTGTGAATGCTCTTTCAAAATCAGTTTATGAAAAGTTGTTCTTGTGGATGGTCACTCGCA TTAACCAGCAACTGGATACGAAGCTTCCAAGACAACACTTCATTGGTGTTTTGGACATTGCAGGCTTTGA AATCTTTGAGTATAACAGCCTGGAGCAGCTGTGCATCAACTTCACCAATGAGAAACTGCAACAGTTTTTC AACCACCACATGTTCGTGCTGGAGCAGGAGGAGTACAAGAAGGAAGGCATCGAGTGGACGTTCATTGACT TCGGGATGGACCTGGCTGCCTGCATCGAGCTCATCGAGAAGCCTATGGGCATCTTCTCCATCCTGGAAGA GGAGTGCATGTTCCCCAAGGCAACAGACACCTCCTTCAAGAACAAGCTGTATGACCAGCATCTTGGAAAG TCCAACAACTTCCAGAAGCCCAAGGTGGTCAAAGGCAGGGCCGAGGCTCACTTCTCACTGATCCACTATG CGGGCACCGTGGACTACAGTGTCTCAGGTTGGCTGGAGAAGAACAAGGACCCTCTGAACGAGACTGTGGT TGGGCTGTACCAGAAGTCTTCCAACAGGCTCCTGGCACACCTCTATGCCACGTTTGCCACGGCGGATGCT GACAGTGGAAAGAAGAAAGTTGCCAAGAAGAAGGGTTCTTCCTTCCAAACTGTCTCTGCCCTTTTCAGGG AAAACCTGAACAAGCTGATGTCAAATTTAAGAACTACTCACCCTCATTTTGTGCGTTGTATAATTCCCAA TGAAACCAAAACTCCAGGGGCTATGGAACACAGCCTTGTTCTGCACCAGCTGCGGTGTAACGGTGTCCTG GAGGGCATCCGCATCTGCAGGAAAGGGTTCCCAAACAGGATTCTCTATGGCGATTTTAAACAAAGATACC GAGTGCTGAATGCCAGTGCAATCCCTGAGGGACAATTCATTGACAGCAAGAAAGCCTGTGAAAAGCTTCT GGCATCCATTGATATTGACCACACTCAGTACAAATTTGGACATACCAAGGTGTTCTTCAAGGCTGGCTTG CTGGGAACCCTGGAAGAGATGCGGGATGACCGCCTGGCCAAACTAATCACCCGGACACAAGCTGTGTGCA GAGGGTTCCTCATGCGTGTGGAATTCCAGAAGATGGTGCAGAGGAGGGAGTCCATCTTCTGCATCCAGTA CAACATTCGCTCATTCATGAACGTCAAGCACTGGCCCTGGATGAAACTCTTCTTCAAGATCAAGCCCCTC CTCAAGAGTGCAGAGACTGAGAAAGAGATGGCCACCATGAAGGAAGAATTCCAGAAAACCAAAGATGAAC TCGCCAAGTCGGAGGCAAAAAGGAAGGAGCTAGAGGAAAAACTGGTGACTCTGGTCCAAGAGAAGAATGA CCTGCAGCTCCAAGTACAAGCTGAAAGCGAAAATTTGTTGGATGCTGAGGAAAGATGCGATCAGCTGATC AAAGCCAAATTCCAGCTCGAGGCCAAGATCAAGGAGGTGACAGAGAGAGCTGAAGATGAGGAGGAGATCA ATGCTGAGCTGACGGCCAAGAAGAGGAAACTGGAGGATGAATGCTCAGAGCTCAAGAAAGACATTGATGA CCTTGAGTTGACCCTGGCCAAGGTTGAGAAGGAGAAGCATGCCACAGAGAACAAGGTTAAAAACCTTACT GAGGAACTCTCTGGGTTAGATGAAACAATTGCAAAGTTAACCAGAGAGAAGAAGGCCCTCCAAGAGGCGC ACCAGCAGGCCTTGGATGACCTCCAAGCTGAAGAAGACAAAGTCAATTCTTTGAACAAAACCAAGAGCAA ACTGGAACAGCAAGTGGAAGACCTGGAAAGCTCCCTAGAACAAGAAAAGAAGCTCCGAGTAGACCTGGAA AGGAACAAAAGGAAATTGGAAGGAGACTTGAAGCTTGCTCAAGAGTCCATATTAGATCTGGAGAATGACA AGCAACAGCTGGACGAAAGGCTCAAGAAGAAAGATTTTGAATATTGTCAACTTCAAAGCAAAGTGGAAGA TGAGCAGACACTGGGCCTCCAGTTTCAGAAGAAAATCAAAGAGTTGCAGGCTCGAATTGAGGAGCTGGAA GAGGAGATAGAGGCGGAGAGGGCCACCCGCGCGAAGACAGAGAAACAGCGCAGCGACTATGCCCGGGAGC TGGAGGAGCTGAGCGAGCGGCTGGAGGAGGCGGGAGGCGTCACCTCCACGCAGATAGAGCTCAACAAGAA GCGGGAGGCGGAGTTCCTGAAGCTGCGCAGGGACCTGGAGGAGGCCACACTGCAGCACGAAGCCATGGTG GCCGCGCTGAGGAAGAAGCATGCGGATAGTGTGGCCGAGCTTGGGGAGCAGATTGACAACCTGCAGCGGG TCAAGCAGAAGCTGGAGAAGGAGAAGAGCGAGTTCAAGCTGGAGATCGATGACCTCTCCAGCAGCATGGA GAGTGTGTCGAAATCTAAGGCAAATCTGGAAAAAATCTGCCGAACCCTGGAGGATCAGTTAAGTGAGGCC AGGGGCAAGAATGAGGAAATTCAGAGGAGCCTGAGCGAGCTGACCACACAGAAGTCTCGTTTGCAGACCG AGGCTGGTGAGCTGAGTCGTCAGCTGGAAGAAAAAGAAAGCATAGTATCCCAACTTTCCAGGAGCAAGCA AGCCTTTACCCAGCAAACAGAAGAGCTCAAGAGGCAGCTGGAGGAAGAGAACAAGGCCAAGAACGCCCTG GCGCACGCCCTGCAGTCCTCCCGCCACGACTGTGACCTGCTGCGGGAACAGTATGAGGAGGAGCAGGAAG GCAAAGCTGAGCTGCAGAGGGCGCTGTCCAAGGCCAATAGTGAGGTTGCCCAGTGGAGAACCAAATACGA GACGGACGCCATCCAGCGCACAGAAGAGCTGGAGGAGGCCAAGAAAAAACTTGCTCAGCGCCTTCAAGAT TCCGAGGAACAGGTTGAGGCAGTGAATGCTAAATGTGCTTCACTGGAGAAGACCAAGCAGAGGCTGCAAG GAGAGGTGGAGGATCTGATGGTTGATGTTGAAAGAGCCAATTCCTTGGCCGCCGCTCTGGACAAGAAGCA GAGGAACTTTGACAAGGTGTTGGCAGAGTGGAAGACAAAGTGTGAGGAGAGCCAAGCAGAGCTGGAGGCA TCCCTGAAGGAGTCCCGCTCCTTGAGCACTGAGCTCTTCAAACTGAAAAATGCCTACGAGGAAGCCTTAG ATCAACTTGAAACTGTGAAACGGGAAAATAAGAACTTAGAGCAGGAGATAGCAGATCTCACAGAACAAAT TGCTGAAAATGGCAAAACCATCCATGAACTGGAGAAATCAAGAAAGCAGATTGAGCTGGAAAAGGCTGAT ATCCAGCTGGCTCTCGAGGAAGCAGAGGCTGCTCTTGAGCATGAAGAAGCCAAGATCCTCCGAATCCAGC TTGAATTGACACAAGTGAAATCAGAAATTGATAGAAAGATCGCCGAGAAGGATGAAGAGATCGAGCAGCT GAAGAGGAACTACCAGAGAACAGTGGAAACCATGCAGAGCGCCCTGGACGCCGAGGTGCGGAGCAGGAAT GAAGCCATCCGGCTCAAGAAGAAGATGGAGGGGGACCTGAATGAAATCGAGATCCAGCTGAGCCACGCCA ACCGCCAGGCGGCGGAGACCCTCAAACACCTCAGGAGTGTCCAGGGACAGCTGAAGGATACGCAGCTCCA CCTGGATGATGCCCTCCGGGGCCAGGAGGACCTGAAGGAGCAGCTGGCGATTGTGGAGCGCAGAGCCAAC CTGCTGCAGGCCGAGGTGGAGGAGCTGCGGGCTACTCTGGAGCAGACGGAGAGGGCCCGGAAACTGGCGG AACAGGAGCTCCTGGACTCCAACGAGAGGGTGCAGCTGCTGCATACCCAGAACACCAGCCTCATCCACAC CAAGAAGAAGCTGGAGACAGACCTCATGCAGCTCCAGAGTGAGGTAGAAGATGCCAGCAGGGATGCAAGG AACGCTGAGGAGAAGGCCAAGAAGGCCATCACGGACGCTGCCATGATGGCGGAGGAGCTGAAGAAGGAGC AGGACACCAGCGCCCACCTTGAGCGGATGAAGAAGAACCTGGAACAGACGGTGAAGGACCTGCAGCATCG TCTAGATGAGGCCGAGCAGCTGGCGCTGAAGGGCGGGAAGAAGCAGATCCAGAAACTGGAGACCAGGATC CGAGAGCTGGAGTTTGAACTTGAGGGAGAGCAGAAGAAGAACACAGAGTCTGTTAAGGGCCTGAGGAAGT ATGAGCGGAGGGTCAAGGAGCTGACGTACCAGAGTGAAGAGGACAGGAAGAATGTGCTGAGATTGCAGGA TCTGGTGGATAAACTGCAAGTGAAAGTCAAGTCCTACAAGAGGCAGGCGGAGGAGGCTGATGAACAAGCC AATGCTCATCTCACCAAATTCCGAAAGGCTCAGCATGAGCTGGAGGAGGCCGAGGAACGTGCGGATATCG CAGAATCTCAAGTCAACAAGCTCCGCGCTAAGACTCGAGACTTCACCTCCAGCAGGATGGTGGTCCACGA GAGTGAAGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC218098 representing NM_002470
Red=Cloning site Green=Tags(s) MSSDTEMEVFGIAAPFLRKSEKERIEAQNQPFDAKTYCFVVDSKEEYAKGKIKSSQDGKVTVETEDNRTL VVKPEDVYAMNPPKFDRIEDMAMLTHLNEPAVLYNLKDRYTSWMIYTYSGLFCVTVNPYKWLPVYNPEVV EGYRGKKRQEAPPHIFSISDNAYQFMLTDRENQSILITGESGAGKTVNTKRVIQYFATIAATGDLAKKKD SKMKGTLEDQIISANPLLEAFGNAKTVRNDNSSRFGKFIRIHFGTTGKLASADIETYLLEKSRVTFQLKA ERSYHIFYQILSNKKPELIELLLITTNPYDYPFISQGEILVASIDDAEELLATDSAIDILGFTPEEKSGL YKLTGAVMHYGNMKFKQKQREEQAEPDGTEVADKTAYLMGLNSSDLLKALCFPRVKVGNEYVTKGQTVDQ VHHAVNALSKSVYEKLFLWMVTRINQQLDTKLPRQHFIGVLDIAGFEIFEYNSLEQLCINFTNEKLQQFF NHHMFVLEQEEYKKEGIEWTFIDFGMDLAACIELIEKPMGIFSILEEECMFPKATDTSFKNKLYDQHLGK SNNFQKPKVVKGRAEAHFSLIHYAGTVDYSVSGWLEKNKDPLNETVVGLYQKSSNRLLAHLYATFATADA DSGKKKVAKKKGSSFQTVSALFRENLNKLMSNLRTTHPHFVRCIIPNETKTPGAMEHSLVLHQLRCNGVL EGIRICRKGFPNRILYGDFKQRYRVLNASAIPEGQFIDSKKACEKLLASIDIDHTQYKFGHTKVFFKAGL LGTLEEMRDDRLAKLITRTQAVCRGFLMRVEFQKMVQRRESIFCIQYNIRSFMNVKHWPWMKLFFKIKPL LKSAETEKEMATMKEEFQKTKDELAKSEAKRKELEEKLVTLVQEKNDLQLQVQAESENLLDAEERCDQLI KAKFQLEAKIKEVTERAEDEEEINAELTAKKRKLEDECSELKKDIDDLELTLAKVEKEKHATENKVKNLT EELSGLDETIAKLTREKKALQEAHQQALDDLQAEEDKVNSLNKTKSKLEQQVEDLESSLEQEKKLRVDLE RNKRKLEGDLKLAQESILDLENDKQQLDERLKKKDFEYCQLQSKVEDEQTLGLQFQKKIKELQARIEELE EEIEAERATRAKTEKQRSDYARELEELSERLEEAGGVTSTQIELNKKREAEFLKLRRDLEEATLQHEAMV AALRKKHADSVAELGEQIDNLQRVKQKLEKEKSEFKLEIDDLSSSMESVSKSKANLEKICRTLEDQLSEA RGKNEEIQRSLSELTTQKSRLQTEAGELSRQLEEKESIVSQLSRSKQAFTQQTEELKRQLEEENKAKNAL AHALQSSRHDCDLLREQYEEEQEGKAELQRALSKANSEVAQWRTKYETDAIQRTEELEEAKKKLAQRLQD SEEQVEAVNAKCASLEKTKQRLQGEVEDLMVDVERANSLAAALDKKQRNFDKVLAEWKTKCEESQAELEA SLKESRSLSTELFKLKNAYEEALDQLETVKRENKNLEQEIADLTEQIAENGKTIHELEKSRKQIELEKAD IQLALEEAEAALEHEEAKILRIQLELTQVKSEIDRKIAEKDEEIEQLKRNYQRTVETMQSALDAEVRSRN EAIRLKKKMEGDLNEIEIQLSHANRQAAETLKHLRSVQGQLKDTQLHLDDALRGQEDLKEQLAIVERRAN LLQAEVEELRATLEQTERARKLAEQELLDSNERVQLLHTQNTSLIHTKKKLETDLMQLQSEVEDASRDAR NAEEKAKKAITDAAMMAEELKKEQDTSAHLERMKKNLEQTVKDLQHRLDEAEQLALKGGKKQIQKLETRI RELEFELEGEQKKNTESVKGLRKYERRVKELTYQSEEDRKNVLRLQDLVDKLQVKVKSYKRQAEEADEQA NAHLTKFRKAQHELEEAEERADIAESQVNKLRAKTRDFTSSRMVVHESEE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_002470 |
| ORF Size | 5820 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_002470.4 |
| RefSeq Size | 6061 bp |
| RefSeq ORF | 5823 bp |
| Locus ID | 4621 |
| UniProt ID | P11055 |
| Protein Pathways | Tight junction, Viral myocarditis |
| MW | 224.4 kDa |
| Gene Summary | Myosin is a major contractile protein which converts chemical energy into mechanical energy through the hydrolysis of ATP. Myosin is a hexameric protein composed of a pair of myosin heavy chains (MYH) and two pairs of nonidentical light chains. This gene is a member of the MYH family and encodes a protein with an IQ domain and a myosin head-like domain. Mutations in this gene have been associated with two congenital contracture (arthrogryposis) syndromes, Freeman-Sheldon syndrome and Sheldon-Hall syndrome. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC218098L1 | Lenti ORF clone of Human myosin, heavy chain 3, skeletal muscle, embryonic (MYH3), Myc-DDK-tagged |
CNY 20,890.00 |
|
| RG218098 | MYH3 (tGFP-tagged) - Human myosin, heavy chain 3, skeletal muscle, embryonic (MYH3) |
CNY 21,160.00 |
|
| SC123997 | MYH3 (untagged)-Human myosin, heavy chain 3, skeletal muscle, embryonic (MYH3) |
CNY 13,048.00 |
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