United States
Germany
Japan
United Kingdom
China
DENN (MADD) (NM_130473) Human Tagged Lenti ORF Clone
CAT＃: RC219233L3
- LentiORF®
Lenti-ORF clone of MADD (Myc-DDK-tagged)-Human MAP-kinase activating death domain (MADD), transcript variant 5
"NM_130473" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 17,960.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | DEEAH; DENN; IG20; NEDDISH; RAB3GEP; RabGEF |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC219233).
|
| Restriction Sites |
SgfI-RsrII
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_130473 |
| ORF Size | 4824 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_130473.2, NP_569829.2 |
| RefSeq Size | 5914 bp |
| RefSeq ORF | 4827 bp |
| Locus ID | 8567 |
| UniProt ID | Q8WXG6 |
| Protein Families | Druggable Genome |
| MW | 178.6 kDa |
| Gene Summary | Tumor necrosis factor alpha (TNF-alpha) is a signaling molecule that interacts with one of two receptors on cells targeted for apoptosis. The apoptotic signal is transduced inside these cells by cytoplasmic adaptor proteins. The protein encoded by this gene is a death domain-containing adaptor protein that interacts with the death domain of TNF-alpha receptor 1 to activate mitogen-activated protein kinase (MAPK) and propagate the apoptotic signal. It is membrane-bound and expressed at a higher level in neoplastic cells than in normal cells. Several transcript variants encoding different isoforms have been described for this gene. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219233 | MADD (Myc-DDK-tagged)-Human MAP-kinase activating death domain (MADD), transcript variant 5 |
CNY 16,060.00 |
|
| RC219233L4 | Lenti-ORF clone of MADD (mGFP-tagged)-Human MAP-kinase activating death domain (MADD), transcript variant 5 |
CNY 17,960.00 |
|
| RG219233 | MADD (tGFP-tagged) - Human MAP-kinase activating death domain (MADD), transcript variant 5 |
CNY 17,670.00 |
|
| SC309591 | MADD (untagged)-Human MAP-kinase activating death domain (MADD), transcript variant 5 |
CNY 31,070.00 |
