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TRPV5 (NM_019841) Human Tagged ORF Clone
CAT＃: RC219654
- TrueORF®
TRPV5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5)
ORF Plasmid: tGFP
"NM_019841" in other vectors (6)
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CNY 8,008.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | CAT2; ECAC1; OTRPC3 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC219654 representing NM_019841
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGGGGGTTTTCTACCTAAGGCAGAAGGGCCCGGGAGCCAACTCCAGAAACTTCTGCCCTCCTTTCTGG TCAGAGAACAAGACTGGGACCAGCACCTGGACAAGCTTCATATGCTGCAGCAGAAGAGGATTCTAGAGTC TCCACTGCTTCGAGCATCCAAGGAAAATGACCTGTCTGTTCTTAGGCAACTTCTACTGGACTGCACCTGT GACGTTCGACAAAGAGGAGCCCTGGGGGAGACGGCGCTGCACATAGCAGCCCTCTATGACAACTTGGAGG CGGCCTTGGTGCTGATGGAGGCTGCCCCAGAGCTGGTCTTTGAGCCCACCACATGTGAGGCTTTTGCAGG TCAGACTGCACTGCACATCGCTGTTGTGAACCAGAATGTGAACCTGGTGCGTGCCCTGCTCACCCGCAGG GCCAGTGTCTCTGCCAGAGCCACAGGCACTGCCTTCCGCCGTAGTCCCCGCAACCTCATCTACTTTGGGG AGCACCCTTTGTCCTTTGCTGCCTGTGTGAACAGCGAGGAGATCGTGCGGCTGCTCATTGAGCATGGAGC TGACATCAGGGCCCAGGACTCCCTGGGAAACACAGTATTACACATCCTCATCCTCCAGCCCAACAAAACC TTTGCCTGCCAGATGTACAACCTGCTGCTGTCCTACGATGGACATGGGGACCACCTGCAGCCCCTGGACC TTGTGCCCAATCACCAGGGTCTCACCCCCTTCAAGCTGGCTGGAGTGGAGGGTAACACTGTGATGTTCCA GCACCTGATGCAGAAGCGGAGGCACATCCAGTGGACGTATGGACCCCTGACCTCCATTCTCTACGACCTC ACAGAGATCGACTCCTGGGGAGAGGAGCTGTCCTTCCTGGAGCTTGTGGTCTCCTCTGATAAACGAGAGG CTCGCCAAATTCTGGAACAGACCCCAGTGAAGGAGCTGGTGAGCTTCAAGTGGAACAAGTATGGCCGGCC GTACTTCTGCATCCTGGCTGCCTTGTACCTGCTCTACATGATCTGCTTTACCACGTGCTGCGTCTACCGC CCCCTTAAGTTTCGTGGTGGCAACCGCACTCATTCTCGAGACATCACCATCCTCCAGCAAAAACTACTAC AGGAGGCCTATGAGACACGTGAAGATATCATCAGGCTGGTGGGGGAGCTGGTGAGCATCGTTGGGGCTGT GATCATCCTGCTCCTAGAGATTCCAGACATCTTCAGGGTTGGTGCCTCTCGCTATTTTGGAAAGACGATT CTTGGGGGGCCATTCCATGTCATCATCATCACCTATGCCTCCCTGGTGCTGGTGACCATGGTGATGCGGC TCACCAACACCAATGGGGAGGTGGTGCCCATGTCCTTTGCCCTGGTGCTGGGCTGGTGCAGTGTCATGTA TTTCACTCGAGGATTCCAGATGCTGGGTCCCTTCACCATCATGATCCAGAAGATGATTTTTGGAGACCTA ATGCGTTTCTGCTGGCTGATGGCTGTGGTCATCTTGGGATTTGCCTCCGCGTTCTATATCATTTTCCAGA CAGAGGACCCAACCAGTCTGGGGCAATTCTATGACTACCCCATGGCACTGTTCACCACCTTTGAGCTTTT TCTCACTGTTATTGATGCACCTGCCAACTACGACGTGGACTTGCCCTTCATGTTCAGCATTGTCAACTTC GCCTTCGCCATCATTGCCACACTGCTCATGCTCAACTTGTTCATCGCCATGATGGGCGACACCCACTGGA GGGTGGCCCAGGAGAGGGATGAGCTCTGGAGGGCCCAGGTCGTGGCCACCACAGTGATGCTGGAGCGGAA GCTGCCTCGCTGCCTGTGGCCTCGCTCCGGGATCTGTGGGTGCGAATTCGGGCTGGGGGACCGCTGGTTC CTGCGGGTTGAGAACCACAATGATCAGAATCCTCTGCGAGTGCTTCGCTATGTGGAAGTGTTCAAGAACT CAGACAAGGAGGATGACCAGGAGCATCCATCTGAGAAACAGCCCTCTGGGGCTGAGAGTGGGACTCTAGC CAGAGCCTCTTTGGCTCTTCCAACTTCCTCCCTGTCCCGGACCGCGTCCCAGAGCAGCAGTCACCGAGGC TGGGAGATCCTTCGTCAAAACACCCTGGGGCACTTGAATCTTGGACTGAACCTTAGTGAGGGGGATGGAG AGGAGGTCTACCATTTT ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC219654 representing NM_019841
Red=Cloning site Green=Tags(s) MGGFLPKAEGPGSQLQKLLPSFLVREQDWDQHLDKLHMLQQKRILESPLLRASKENDLSVLRQLLLDCTC DVRQRGALGETALHIAALYDNLEAALVLMEAAPELVFEPTTCEAFAGQTALHIAVVNQNVNLVRALLTRR ASVSARATGTAFRRSPRNLIYFGEHPLSFAACVNSEEIVRLLIEHGADIRAQDSLGNTVLHILILQPNKT FACQMYNLLLSYDGHGDHLQPLDLVPNHQGLTPFKLAGVEGNTVMFQHLMQKRRHIQWTYGPLTSILYDL TEIDSWGEELSFLELVVSSDKREARQILEQTPVKELVSFKWNKYGRPYFCILAALYLLYMICFTTCCVYR PLKFRGGNRTHSRDITILQQKLLQEAYETREDIIRLVGELVSIVGAVIILLLEIPDIFRVGASRYFGKTI LGGPFHVIIITYASLVLVTMVMRLTNTNGEVVPMSFALVLGWCSVMYFTRGFQMLGPFTIMIQKMIFGDL MRFCWLMAVVILGFASAFYIIFQTEDPTSLGQFYDYPMALFTTFELFLTVIDAPANYDVDLPFMFSIVNF AFAIIATLLMLNLFIAMMGDTHWRVAQERDELWRAQVVATTVMLERKLPRCLWPRSGICGCEFGLGDRWF LRVENHNDQNPLRVLRYVEVFKNSDKEDDQEHPSEKQPSGAESGTLARASLALPTSSLSRTASQSSSHRG WEILRQNTLGHLNLGLNLSEGDGEEVYHF TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_019841 |
| ORF Size | 2187 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_019841.4, NP_062815.2 |
| RefSeq Size | 2486 bp |
| RefSeq ORF | 2190 bp |
| Locus ID | 56302 |
| UniProt ID | Q9NQA5 |
| Protein Families | Druggable Genome, Ion Channels: Transient receptor potential, Transmembrane |
| MW | 83 kDa |
| Gene Summary | This gene is a member of the transient receptor family and the TrpV subfamily. The calcium-selective channel encoded by this gene has 6 transmembrane-spanning domains, multiple potential phosphorylation sites, an N-linked glycosylation site, and 5 ANK repeats. This protein forms homotetramers or heterotetramers and is activated by a low internal calcium level. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC219654L1 | Lenti-ORF clone of TRPV5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 10,408.00 |
|
| RC219654L2 | Lenti-ORF clone of TRPV5 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 7,700.00 |
|
| RC219654L3 | Lenti-ORF clone of TRPV5 (Myc-DDK-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 7,700.00 |
|
| RC219654L4 | Lenti-ORF clone of TRPV5 (mGFP-tagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 7,700.00 |
|
| RG219654 | TRPV5 (tGFP-tagged) - Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 9,608.00 |
|
| SC122108 | TRPV5 (untagged)-Human transient receptor potential cation channel, subfamily V, member 5 (TRPV5) |
CNY 8,016.00 |
