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ARMET (MANF) (NM_006010) Human Tagged Lenti ORF Clone
CAT＃: RC221555L2
- LentiORF®
Lenti ORF clone of Human mesencephalic astrocyte-derived neurotrophic factor (MANF), mGFP tagged
"NM_006010" in other vectors (6)
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CNY 4,800.00
CNY 5,890.00
Product images
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | mGFP |
| Synonyms | ARMET; ARP |
| Vector | pLenti-C-mGFP |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | None |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC221555).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_006010 |
| ORF Size | 555 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_006010.5, NP_006001.4 |
| RefSeq Size | 993 bp |
| RefSeq ORF | 549 bp |
| Locus ID | 7873 |
| UniProt ID | P55145 |
| Protein Families | Druggable Genome, Secreted Protein |
| MW | 21.6 kDa |
| Gene Summary | The protein encoded by this gene is localized in the endoplasmic reticulum (ER) and golgi, and is also secreted. Reducing expression of this gene increases susceptibility to ER stress-induced death and results in cell proliferation. Activity of this protein is important in promoting the survival of dopaminergic neurons. The presence of polymorphisms in the N-terminal arginine-rich region, including a specific mutation that changes an ATG start codon to AGG, have been reported in a variety of solid tumors; however, these polymorphisms were later shown to exist in normal tissues and are thus no longer thought to be tumor-related. [provided by RefSeq, Apr 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC221555 | MANF (Myc-DDK-tagged)-Human mesencephalic astrocyte-derived neurotrophic factor (MANF) |
CNY 2,400.00 |
|
| RC221555L1 | Lenti ORF clone of Human mesencephalic astrocyte-derived neurotrophic factor (MANF), Myc-DDK-tagged |
CNY 4,800.00 |
|
| RC221555L3 | Lenti ORF clone of Human mesencephalic astrocyte-derived neurotrophic factor (MANF), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC221555L4 | Lenti ORF clone of Human mesencephalic astrocyte-derived neurotrophic factor (MANF), mGFP tagged |
CNY 5,890.00 |
|
| RG221555 | MANF (tGFP-tagged) - Human mesencephalic astrocyte-derived neurotrophic factor (MANF) |
CNY 4,000.00 |
|
| SC122734 | MANF (untagged)-Human mesencephalic astrocyte-derived neurotrophic factor (MANF) |
CNY 2,400.00 |
