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SP3 (NM_003111) Human Tagged ORF Clone
CAT＃: RC222027
SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 1
ORF Plasmid: tGFP
"NM_003111" in other vectors (6)
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CNY 3,990.00
| Cited in 3 publications. |
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SPR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC222027 representing NM_003111
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCTCCCGAAAAGCCCGTGAAACAAGAGGAAATGGCTGCCTTGGACGTGGATAGCGGCGGCGGCG GTGGCGGCGGCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCACGGAAACGGTGCGGTGGCGGC GGCAGCGGCGGCCCAGGACACTCAGCCGTCACCGCTCGCTCTGCTGGCCGCTACCTGCAGCAAGATAGGG CCGCCATCGCCGGGCGACGACGAGGAGGAGGCGGCCGCCGCAGCCGGGGCCCCCGCCGCCGCCGGAGCGA CAGGTGATTTGGCTTCTGCACAGTTAGGAGGAGCACCAAACCGATGGGAGGTTTTGTCAGCCACACCTAC AACTATAAAAGATGAAGCTGGTAATCTAGTCCAGATTCCAAGTGCTGCTACTTCAAGTGGGCAGTATGTT CTTCCCCTTCAGAATTTGCAGAATCAACAAATATTTTCCGTTGCACCAGGATCAGATTCATCAAATGGTG CAGTGTCCAGTGTTCAATATCAAGTGATACCACAGATCCAGTCAGCAGATGGTCAGCAGGTTCAAATTGG TTTCACAGGCTCTTCAGATAATGGGGGTATAAATCAAGAAAGCAGTCAAATTCAGATCATTCCTGGCTCT AATCAAACCTTACTTGCCTCTGGAACACCTTCTGCTAACATCCAGAATCTCATACCACAGACTGGTCAAG TCCAGGTTCAGGGAGTTGCAATTGGTGGTTCATCTTTTCCTGGTCAAACCCAAGTAGTTGCTAATGTGCC TCTTGGTCTGCCAGGAAATATTACGTTTGTACCAATCAATAGTGTCGATCTAGATTCTTTGGGACTCTCG GGCAGTTCTCAGACAATGACTGCAGGCATTAATGCCGACGGACATTTGATAAACACAGGACAAGCTATGG ATAGTTCAGACAATTCAGAAAGGACTGGTGAGCGGGTTTCTCCTGATATTAATGAAACTAATACTGATAC AGATTTATTTGTGCCAACATCCTCTTCATCACAGTTGCCTGTTACGATAGATAGTACAGGTATATTACAA CAAAACACAAATAGCTTGACTACATCTAGTGGGCAGGTTCATTCTTCAGATCTTCAGGGAAATTATATCC AGTCGCCTGTTTCTGAAGAGACACAGGCACAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAGCA TCTACAACTTCAAGAGTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACA ATCCATGGTGTGCAAGCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAATCTTCAGTTGCAGCTGA ATCCTGGAACCTTTTTAATTCAGGCACAGACAGTGACCCCTTCTGGACAGGTAACTTGGCAAACGTTTCA AGTACAAGGGGTCCAGAACTTGCAGAATTTGCAAATACAGAATACTGCTGCCCAACAAATAACTTTGACG CCTGTTCAAACCCTCACACTTGGTCAAGTTGCGGCAGGTGGAGCCTTCACTTCAACTCCAGTTAGTCTAA GCACTGGTCAGTTGCCAAATCTACAAACAGTTACAGTGAACTCTATAGATTCTGCTGGTATACAGCTACA TCCAGGAGAGAATGCTGACAGTCCTGCAGATATTAGGATCAAGGAAGAAGAACCTGATCCTGAAGAGTGG CAGCTCAGTGGTGATTCTACCTTGAATACCAATGACCTAACACACTTAAGAGTACAGGTGGTAGATGAAG AAGGGGACCAACAACATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGCACCTGTCCCAACTGTAAAGA AGGTGGTGGAAGAGGTACCAATCTTGGGAAAAAGAAGCAACACATTTGTCATATACCAGGATGTGGTAAA GTCTATGGGAAGACCTCACATCTGAGAGCTCATCTGCGTTGGCATTCTGGAGAACGCCCTTTTGTTTGTA ACTGGATGTACTGTGGTAAAAGATTTACTCGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGG TGAGAAGAAATTTGTTTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATT AAAACACACCAGAATAAAAAAGGTATTCACTCTAGCAGTACAGTGCTGGCATCTGTGGAAGCTGCGCGAG ATGATACTTTGATTACTGCAGGAGGAACAACGCTTATCCTTGCAAATATTCAACAAGGTTCTGTTTCAGG GATAGGAACTGTTAATACTTCCGCCACCAGCAATCAAGATATCCTTACCAACACTGAAATACCTTTACAG CTTGTCACAGTTTCTGGAAATGAGACAATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC222027 representing NM_003111
Red=Cloning site Green=Tags(s) MTAPEKPVKQEEMAALDVDSGGGGGGGGGHGEYLQQQQQHGNGAVAAAAAAQDTQPSPLALLAATCSKIG PPSPGDDEEEAAAAAGAPAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPSAATSSGQYV LPLQNLQNQQIFSVAPGSDSSNGAVSSVQYQVIPQIQSADGQQVQIGFTGSSDNGGINQESSQIQIIPGS NQTLLASGTPSANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLS GSSQTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQ QNTNSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQT IHGVQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQVTWQTFQVQGVQNLQNLQIQNTAAQQITLT PVQTLTLGQVAAGGAFTSTPVSLSTGQLPNLQTVTVNSIDSAGIQLHPGENADSPADIRIKEEEPDPEEW QLSGDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGK VYGKTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHI KTHQNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQ LVTVSGNETME TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_003111 |
| ORF Size | 2343 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_003111.4 |
| RefSeq Size | 3920 bp |
| RefSeq ORF | 2346 bp |
| Locus ID | 6670 |
| UniProt ID | Q02447 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 81.7 kDa |
| Gene Summary | This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010] |
Citations (3)
| The use of this cDNA Clones has been cited in the following citations: |
|---|
|
A type 2 diabetes-associated SNP in KCNQ1 (rs163184) modulates the binding activity of the locus for Sp3 and Lsd1/Kdm1a, potentially affecting CDKN1C expression
,null,
International Journal of Molecular Medicine
,PubMed ID 29207083
[SP3]
|
|
Differential effects of Sp cellular transcription factors on viral promoter activation by varicella-zoster virus (VZV) IE62 protein
,Khalil, MI;Ruyechan, WT;Hay, J;Arvin, A;,
Virology
,PubMed ID 26207799
[SP3]
|
|
Regulation of the Bone-restricted IFITM-like (Bril) Gene Transcription by Sp and Gli Family Members and CpG Methylation
,Bahar Kasaai, Marie-Hélène Gaumond, and Pierre Moffatt,
J. Biol. Chem., May 2013; 288: 13278 - 13294.
,PubMed ID 23530031
[SP3]
|
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC222027L1 | Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222027L2 | Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RC222027L3 | Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC222027L4 | Lenti ORF clone of Human Sp3 transcription factor (SP3), transcript variant 1, mGFP tagged |
CNY 5,890.00 |
|
| RG222027 | SP3 (tGFP-tagged) - Human Sp3 transcription factor (SP3), transcript variant 1 |
CNY 4,370.00 |
|
| SC121408 | SP3 (untagged)-Human Sp3 transcription factor (SP3), transcript variant 1 |
CNY 5,728.00 |
