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EN1 (NM_001426) Human Tagged ORF Clone
CAT＃: RC224659
EN1 (Myc-DDK-tagged)-Human engrailed homeobox 1 (EN1)
ORF Plasmid: tGFP
"NM_001426" in other vectors (4)
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CNY 5,488.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | ENDOVESLB |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC224659 representing NM_001426
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGGAAGAACAGCAGCCGGAACCTAAAAGTCAGCGCGACTCGGCCCTCGGCGGCGCGGCGGCGGCGACTC CGGGCGGCCTCAGCCTGAGCCTCAGTCCGGGCGCCAGCGGCAGCAGCGGCAGCGGCAGCGATGGAGACAG CGTGCCGGTGTCCCCGCAGCCTGCGCCCCCCTCGCCGCCCGCGGCGCCTTGCCTGCCGCCCCTGGCCCAC CACCCGCACCTCCCCCCACACCCCCCGCCCCCGCCGCCTCAGCATCTCGCGGCGCCTGCTCACCAGCCGC AGCCAGCGGCCCAGCTGCACCGCACCACCAACTTTTTCATCGACAACATCCTGAGGCCGGACTTCGGCTG CAAAAAGGAGCAGCCGCCACCGCAGCTTCTGGTGGCTGCGGCGGCCAGAGGAGGCGCAGGAGGAGGAGGC CGGGTCGAGCGTGACAGAGGCCAGACTGCCGCAGGTAGAGACCCTGTCCACCCGTTGGGCACCCGGGCGC CAGGCGCTGCCTCGCTCCTGTGCGCCCCGGACGCGAACTGTGGCCCACCCGACGGCTCCCAGCCAGCCGC CGCCGGCGCGGGCGCGTCTAAAGCTGGGAACCCGGCTGCGGCGGCGGCGGCGGCCGCGGCGGCAGTGGCG GCGGCGGCGGCGGCCGCAGCAGCCAAGCCCTCGGACACCGGTGGCGGCGGCAGTGGAGGCGGCGCGGGGA GCCCCGGAGCGCAGGGCACCAAATACCCGGAGCACGGCAACCCGGCTATCCTACTTATGGGCTCAGCCAA CGGCGGGCCCGTGGTCAAAACTGACTCGCAGCAGCCTCTCGTATGGCCCGCCTGGGTGTACTGCACACGT TATTCGGATCGTCCATCCTCCGGTCCGCGCACCAGGAAGCTGAAGAAGAAGAAGAACGAGAAGGAGGACA AGCGGCCGCGGACCGCGTTCACGGCCGAGCAGCTGCAGAGACTCAAGGCGGAGTTCCAGGCAAACCGCTA CATCACGGAGCAGCGGCGGCAGACCCTGGCCCAGGAACTCAGCCTCAACGAGTCCCAGATCAAGATCTGG TTCCAGAACAAGCGCGCCAAGATCAAGAAAGCCACAGGCATCAAGAACGGCCTGGCGCTGCACCTCATGG CCCAGGGACTGTACAACCACTCCACCACCACGGTCCAGGACAAAGACGAGAGCGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC224659 representing NM_001426
Red=Cloning site Green=Tags(s) MEEQQPEPKSQRDSALGGAAAATPGGLSLSLSPGASGSSGSGSDGDSVPVSPQPAPPSPPAAPCLPPLAH HPHLPPHPPPPPPQHLAAPAHQPQPAAQLHRTTNFFIDNILRPDFGCKKEQPPPQLLVAAAARGGAGGGG RVERDRGQTAAGRDPVHPLGTRAPGAASLLCAPDANCGPPDGSQPAAAGAGASKAGNPAAAAAAAAAAVA AAAAAAAAKPSDTGGGGSGGGAGSPGAQGTKYPEHGNPAILLMGSANGGPVVKTDSQQPLVWPAWVYCTR YSDRPSSGPRTRKLKKKKNEKEDKRPRTAFTAEQLQRLKAEFQANRYITEQRRQTLAQELSLNESQIKIW FQNKRAKIKKATGIKNGLALHLMAQGLYNHSTTTVQDKDESE TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Chromatograms |
CHROMATOGRAMS
 Sequencher program is needed, download here. |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001426 |
| ORF Size | 1176 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001426.2, NP_001417.2 |
| RefSeq Size | 2943 bp |
| RefSeq ORF | 1179 bp |
| Locus ID | 2019 |
| UniProt ID | Q05925 |
| Protein Families | Druggable Genome, ES Cell Differentiation/IPS |
| MW | 39.9 kDa |
| Gene Summary | Homeobox-containing genes are thought to have a role in controlling development. In Drosophila, the 'engrailed' (en) gene plays an important role during development in segmentation, where it is required for the formation of posterior compartments. Different mutations in the mouse homologs, En1 and En2, produced different developmental defects that frequently are lethal. The human engrailed homologs 1 and 2 encode homeodomain-containing proteins and have been implicated in the control of pattern formation during development of the central nervous system. [provided by RefSeq, Jul 2008] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC224659L3 | Lenti ORF clone of Human engrailed homeobox 1 (EN1), Myc-DDK-tagged |
CNY 5,890.00 |
|
| RC224659L4 | Lenti ORF clone of Human engrailed homeobox 1 (EN1), mGFP tagged |
CNY 5,890.00 |
|
| RG224659 | EN1 (tGFP-tagged) - Human engrailed homeobox 1 (EN1) |
CNY 7,088.00 |
|
| SC303016 | EN1 (untagged)-Human engrailed homeobox 1 (EN1) |
CNY 5,488.00 |
