United States
Germany
Japan
United Kingdom
China
ORP150 (HYOU1) (NM_001130991) Human Tagged Lenti ORF Clone
CAT＃: RC226294L3
- LentiORF®
Lenti-ORF clone of HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2
"NM_001130991" in other vectors (4)
Need custom modification / cloning service?
Get a free quote
CNY 9,030.00
Product images
CNY 600.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | GRP-170; Grp170; HSP12A; IMD59; ORP-150; ORP150 |
| Vector | pLenti-C-Myc-DDK-P2A-Puro |
| E. coli Selection | Chloramphenicol (34 ug/mL) |
| Mammalian Cell Selection | Puromycin |
| Sequence Data |
The ORF insert of this clone is exactly the same as(RC226294).
|
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001130991 |
| ORF Size | 2997 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001130991.1 |
| RefSeq ORF | 3000 bp |
| Locus ID | 10525 |
| UniProt ID | Q9Y4L1 |
| Protein Families | Transmembrane |
| MW | 111.34 kDa |
| Gene Summary | The protein encoded by this gene belongs to the heat shock protein 70 family. This gene uses alternative transcription start sites. A cis-acting segment found in the 5' UTR is involved in stress-dependent induction, resulting in the accumulation of this protein in the endoplasmic reticulum (ER) under hypoxic conditions. The protein encoded by this gene is thought to play an important role in protein folding and secretion in the ER. Since suppression of the protein is associated with accelerated apoptosis, it is also suggested to have an important cytoprotective role in hypoxia-induced cellular perturbation. This protein has been shown to be up-regulated in tumors, especially in breast tumors, and thus it is associated with tumor invasiveness. This gene also has an alternative translation initiation site, resulting in a protein that lacks the N-terminal signal peptide. This signal peptide-lacking protein, which is only 3 amino acids shorter than the mature protein in the ER, is thought to have a housekeeping function in the cytosol. In rat, this protein localizes to both the ER by a carboxy-terminal peptide sequence and to mitochondria by an amino-terminal targeting signal. Alternative splicing results in multiple transcript variants. [provided by RefSeq, Mar 2014] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC226294 | HYOU1 (Myc-DDK-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 7,130.00 |
|
| RC226294L4 | Lenti-ORF clone of HYOU1 (mGFP-tagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 9,030.00 |
|
| RG226294 | HYOU1 (tGFP-tagged) - Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 7,890.00 |
|
| SC326310 | HYOU1 (untagged)-Human hypoxia up-regulated 1 (HYOU1), transcript variant 2 |
CNY 19,190.00 |
