SSC5D (NM_001144950) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	S5D-SRCRB
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC226898 representing NM_001144950 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAGGGTCTTGGCCTGCCTCCTTGCGGCCCTGGTGGGGATCCAGGCTGTTGAGCGCCTGCGCCTGGCCG ATGGCCCCCATGGGTGCGCTGGCCGCCTGGAGGTCTGGCATGGCGGGCGCTGGGGCACCGTGTGTGATGA CGGCTGGGACCTGCGCGATGCCGCCGTGGCCTGCCGGCAGCTGGGCTGCGGAGGGGCACTGGCCGCCCCG GGAGGCGCCTTCTTCGGGGAGGGGGCAGGGCCTGTGTGGCTCAGCGAGCTGGCTTGCCGGGGCAACGAGG GGCAGCTGGGCCTCTGCCACCACCGGGGCTGGAAGGCCCACATCTGCTCCCACGAGGAGGACGCGGGCGT CGTCTGCGCAGGTCAGCGTGTGGCTAACTCCAGGGACGACTCAACATCTCCCCTGGATGGGGCTCCCTGG CCAGGGCTGTTGCTGGAGCTGAGCCCCAGCACGGAGGAGCCCCTGGTGACACATGCCCCCCGCCCAGCTG GGAACCCCCAGAACGCCTCCCGGAAGAAGAGCCCCCGGCCCAAGCAGGCCAAGTCCACCCGGGCCCCTCT GCTGACGACAGGAGCCCCCCGCCAAGAGCGGCTGCGCCTGGTCTCTGGCCCCCACAGGTGCGCCGGACGC CTGGAGGTCTGGCACGGCGGGCGCTGGGGCACCGTATGTGACGATGGCTGGGACCTGCGCGACGCTGCTG TAGCCTGCCGGGAACTGGGCTGTGGGGGGGCGCTGGCTGCCCCCGGCGGTGCCAGATTCGGGCCTGGTGC AGGGCCCGTGTGGATGGACGATGTGGGGTGTGGAGGAGGAGAACAGGCCCTCCGAGACTGCCCCCGAAGC CCCTGGGGCCGGAGCAACTGTGACCACAGCGAGGATGCGGGGCTGGTCTGCACCGGCCCAGCACCTCGGC TGCGCCTGGCCGATGGCCCCCACGGGTGCGCCGGCCGCCTGGAGGTCTGGCACGGGGGTCGCTGGGGGTC GGTGTGTGACGACGCCTGGGACCTGCGAGACGCCGCTGTGGCCTGCCGAGAGCTGGGCTGCGGAGGGGCG CTGGCCGCCCCCGGGGGCGCCTTCTTTGGGGAGGGGTCTGGACCCATCATCCTGGACGACCTTCGGTGTC GGGGAAACGAGACGGCCTTACGATTCTGCCCAGCTCGGCCCTGGGGCCAGCATGACTGTCACCACCGCGA GGACGCCGGGGCCGTGTGTGACGGCATGCCCCTGGGCTACGTCCCTCCCACGGCCCCCACGGACAGCAAC AACTCCACGCCCAGGGAGGCTGCCTCCAGGCCCCCGTCCACCATGACGAGCCAGGCTCCAGGGACGGCAG GCGTTTCACCTCCTCCAGCCTCCCCTACTGTCCTTTGGGAGCCTGGACCGGAAGCCGGGTCCCCCCAGCT GCGCCTGGTGGCTGGGCCCAGCAAGTGCTCAGGTCGACTGGAGGTGTGGCATGACCAGCGCTGGGGGACC GTGTGTGACGATAGCTGGGACATGCGGGATTCAGCTGTGGTCTGCCGGGAGCTGGGCTGTGGTGGACCTC AGCAGCCAGACCCTGCTGCTGGCCGCTTTGGCTGGGGTGCGGGCCCCATCTGGCTAGATGATGTGGGCTG TGTGGGGACCGAGGCTTCACTGTCCGACTGCCCTGCTGCTCCCTGGGGAAAGCACAACTGCGCTCACAAT GAGGATGTTGGGGTCACCTGCACTGGGCCCCCAGGCCTGGACTCCATCTCAGACCCCTTCAGCTGGAGCT GGATTCCTGGACTGGGGAGAGATCGGGATGCCTGGCTCCCGGGAGAGCTGGCCACCAAGCCCTCTGCAAG TGTGACTGCCAGTGTTCTGGAGAAAACAACCACGAAGGCCCCAGGGAAAATGCCTAAGAGTACTAAGAAG TGGGTGACAAAAAATGCAAAGAGACCAACCACTCAACCCCCAGTGATGCCAACCACGAAACACTCCAGGG CCCAAAGCCCCCCAGACCTAACCTCACAGACCACTGCAGCACTGACCACTGAGGCCTCCCGAAGACCTAC CTCTGAGTTTACCAGAAGGCCGACCACGGAGGCCCCCCAGAGATGGACCTCTCACACCACTGCCACGCTG ACCCCTCAGGCCCCCCGAGAACGGACCACTAAGACCATGGCAATGCTGACCACTCAAGGCCCCCAAGAAA TGACCTCTGAGTCCACTATCAAGAGTATCCCTCAGGCCTCCCTGGAGCCATCTGCTGAGATCCCAGAAGG GTCTCCAGAGTCACCCAAAGACCCGGCCCCCTCTCCCAGTGTTAGCACCACTGGGGAATCAGGCCTGTTC CGGGTTCGTCTGGCCGATGGGCCCAACCGCTGTGCTGGCCGGCTGGAAGTGTGGCATGCCGGACGCTGGG GAACAGTGTGTGATGACAACTGGGACCTGCGGGACGCCACTGTGGCCTGCTGGGAACTGGGCTGTGGAAA GGTCCGGCCTCGAGTAGGCAAAACCCATTACGGCCCTGGGACTGGGCCCATCTGGCTGGATGACATGGGC TGTAAGGGAAGCGAGGCCTCACTGAGCGACTGCCCCTCGGGGGCTTGGGGGAAGCACAACTGTGACCACG AGGAAGACGTGGGGCTCACCTGCACTGGCTACACAGACTATGACGATTATCCCCCCTGGACCTGGGACCC CACCTCAAGAGAGGACCTGGCCAAGGGGACTACCACAGCGGGGGTACCTGGACACACTCTCCCCTGGAGG ACCACCCGGCGCCCGGGTAGCTCCTCCCCAGCAATAAGGCGCCTGCCGGACACAGGCAGCAAAGATGGTT ACAAGCTTCCCTGGACGTGGGACACACCATCAGGAAGGGGCCTGGCTGAGGGGACCCCTACCGCAGGCAA ACTAGGACCAACTCTTGGGGCTGGCACCACCAGGAGCCCAGGCAGTCCTCCAACTCTGAGAGTCCATGGA GACACAGGTTCCCCGAGGAAACCGTGGCCCGAGCGCCGGCCACCGCGGCCCGCTGCGACCAGGACAGCGC CCCCAACCCCGTCCCCAGGTCCCTCCGCCTCTCCGGGACCCCCAGGCCCAGCGCTGACCTCTGACTCCAG TCGAGAGCTCACTCCCCACTCAGCCTTGACGTCCGAGGCGACCTCTGACGCTCCGGACACTTCACCACCC ACCCCAGACCCGGCCTCCCGGACGAACCCCGACCTCATCTTGACAAGCCCTGACTTTGCTTTGTCCACCC CTGACTCCAGTGTGGTTCCCGCGTTGACCCCGGAGCCCTCACCCACGCCCTTACCCACCTTGCCCAAAGA GCTGACCTCTGACCCTTCTACACCGTCGGAGGTGACCAGCCTTTCCCCTACCTCAGAGCAGGTCCCAGAA TCTGACACAACCCCAGATTTGGACACAACTCCATACTCCAGTACAGTCTCAGAATATTCTAGATCCCCAG ACCCCTCCCCAAGCCCTCACCCCACTACTACCCCTGATCCCACCATGGCCCCTGACCCCATCACAACCCT TAACCCTACTGTGACCCCTCACTTCCCTACCACCCCTCACCCCACCACGACCCCTCACCCCACCACCATC ACTCACTCCACCATGATTCCTGACCCCACCACAACCCCTCAACCCTTCACCACCATCACTCACTCCACCA TGATTCCTGACCCCACCACAACCCCTCAACCCTTCACCACCATGCAGCCCACCACAACCCCTCACTCCAC AACCCCTCACCCCACCACGACCCCTCATCCCACCACCATCACTCACTCCACCATGATTCCTGACCCCACC ACAACCCCTCAACCCTTCACCACCATGCAGCCCACCACGATGCCTCATCCCACCACGACCCCTCACCCCA CCACGACTCCTCACCCCACCACAACCCCTCACCCCACCACAACCCCTCACCCCACCATGACTCCTGACCC CACCACGACCCCTTACCCCACCACTACTCCTGATCCCACCACGACCCCTCACCCCACAACTCCTGACCCT TCCTCAACCCCTGTCATCACTACTGTGTCCCTTCCAACCTCCTTGGGGACAGAACTCTCCTCTCCCACTC TAGCACCAACAGTCAAGCCCAGTCTGCACCCCCAGTTGACCTTCACAGCACCTGCCCCTCACACCTCCAC ATCCCAGATACCCACCTTAGAGCCCTCTCCAGCCTTGGAGTCCAGCCCCTCCAGGTCCTCCACAGCCACA AGCATGGACCCACTGTCCACTGAGGACTTCAAGCCACCCAGAAGCCAGAGCCCCAACCTAACCCCTCCAC CCACCCATACCCCACACTCAGCCTCTGACCTTACTGTGTCCCCTGACCCCCTCCTTTCCCCCACAGCCCA CCCCTTGGATCATCCTCCCCTTGACCCCCTCACCCTAGGGCCAACTCCTGGTCAGAGCCCAGGCCCCCAT GGTCCATGTGTGGCCCCAACACCACCTGTAAGGGTCATGGCTTGTGAGCCACCTGCCCTGGTGGAGCTGG TGGCTGCTGTGAGGGATGTGGGTGGTCAGCTGCAGAGACTGACCCAGGTCGTGGAACAGGAGCGGCAGGA GCGCCAAGCCCTGCTGCTGGGGCTGACGCAGCTGGTAGAAGCTGCCCGGGGTCTGGGGCAGCTGGGTGAG GCTGTGAAGAGACTGGCAGAGATGGCCTGGACCACCAGCATGCCTGCACCAACCACCACTACCCCAGAGG AAGAAGAAAGACCCCTGAGGGGAGACGTG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC226898 representing NM_001144950 Red=Cloning site Green=Tags(s) MRVLACLLAALVGIQAVERLRLADGPHGCAGRLEVWHGGRWGTVCDDGWDLRDAAVACRQLGCGGALAAP GGAFFGEGAGPVWLSELACRGNEGQLGLCHHRGWKAHICSHEEDAGVVCAGQRVANSRDDSTSPLDGAPW PGLLLELSPSTEEPLVTHAPRPAGNPQNASRKKSPRPKQAKSTRAPLLTTGAPRQERLRLVSGPHRCAGR LEVWHGGRWGTVCDDGWDLRDAAVACRELGCGGALAAPGGARFGPGAGPVWMDDVGCGGGEQALRDCPRS PWGRSNCDHSEDAGLVCTGPAPRLRLADGPHGCAGRLEVWHGGRWGSVCDDAWDLRDAAVACRELGCGGA LAAPGGAFFGEGSGPIILDDLRCRGNETALRFCPARPWGQHDCHHREDAGAVCDGMPLGYVPPTAPTDSN NSTPREAASRPPSTMTSQAPGTAGVSPPPASPTVLWEPGPEAGSPQLRLVAGPSKCSGRLEVWHDQRWGT VCDDSWDMRDSAVVCRELGCGGPQQPDPAAGRFGWGAGPIWLDDVGCVGTEASLSDCPAAPWGKHNCAHN EDVGVTCTGPPGLDSISDPFSWSWIPGLGRDRDAWLPGELATKPSASVTASVLEKTTTKAPGKMPKSTKK WVTKNAKRPTTQPPVMPTTKHSRAQSPPDLTSQTTAALTTEASRRPTSEFTRRPTTEAPQRWTSHTTATL TPQAPRERTTKTMAMLTTQGPQEMTSESTIKSIPQASLEPSAEIPEGSPESPKDPAPSPSVSTTGESGLF RVRLADGPNRCAGRLEVWHAGRWGTVCDDNWDLRDATVACWELGCGKVRPRVGKTHYGPGTGPIWLDDMG CKGSEASLSDCPSGAWGKHNCDHEEDVGLTCTGYTDYDDYPPWTWDPTSREDLAKGTTTAGVPGHTLPWR TTRRPGSSSPAIRRLPDTGSKDGYKLPWTWDTPSGRGLAEGTPTAGKLGPTLGAGTTRSPGSPPTLRVHG DTGSPRKPWPERRPPRPAATRTAPPTPSPGPSASPGPPGPALTSDSSRELTPHSALTSEATSDAPDTSPP TPDPASRTNPDLILTSPDFALSTPDSSVVPALTPEPSPTPLPTLPKELTSDPSTPSEVTSLSPTSEQVPE SDTTPDLDTTPYSSTVSEYSRSPDPSPSPHPTTTPDPTMAPDPITTLNPTVTPHFPTTPHPTTTPHPTTI THSTMIPDPTTTPQPFTTITHSTMIPDPTTTPQPFTTMQPTTTPHSTTPHPTTTPHPTTITHSTMIPDPT TTPQPFTTMQPTTMPHPTTTPHPTTTPHPTTTPHPTTTPHPTMTPDPTTTPYPTTTPDPTTTPHPTTPDP SSTPVITTVSLPTSLGTELSSPTLAPTVKPSLHPQLTFTAPAPHTSTSQIPTLEPSPALESSPSRSSTAT SMDPLSTEDFKPPRSQSPNLTPPPTHTPHSASDLTVSPDPLLSPTAHPLDHPPLDPLTLGPTPGQSPGPH GPCVAPTPPVRVMACEPPALVELVAAVRDVGGQLQRLTQVVEQERQERQALLLGLTQLVEAARGLGQLGE AVKRLAEMAWTTSMPAPTTTTPEEEERPLRGDV TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001144950
ORF Size	4719 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001144950.2
RefSeq ORF	4722 bp
Locus ID	284297
UniProt ID	A1L4H1
MW	165.6 kDa
Gene Summary	Binds to extracellular matrix proteins. Binds to pathogen-associated molecular patterns (PAMPs) present on the cell walls of Gram-positive and Gram-negative bacteria and fungi, behaving as a pattern recognition receptor (PRR). Induces bacterial and fungal aggregation and subsequent inhibition of PAMP-induced cytokine release. Does not possess intrinsic bactericidal activity. May play a role in the innate defense and homeostasis of certain epithelial surfaces (By similarity).[UniProtKB/Swiss-Prot Function]

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