PPP1R3G (NM_001145115) Human Tagged ORF Clone

CAT#: RC227133

PPP1R3G (Myc-DDK-tagged)-Human protein phosphatase 1, regulatory (inhibitor) subunit 3G (PPP1R3G)

ORF Plasmid: DDK tGFP



  "NM_001145115" in other vectors (4)

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CNY 3,656.00

CNY 3,990.00


货期*
现货

规格
    • 10 ug

Product images

经常一起买 (4)
pCMV6-Entry, mammalian vector with C-terminal Myc- DDK Tag, 10ug
    • 10 ug

CNY 5,420.00


DH5α Chemically Competent Cells (≥10^8 cfu/μg of pUC19 DNA)
    • 5 x 200 ul

CNY 1,280.00


Forward sequencing primer VP1.5, Reverse sequencing primer XL39, 100pmoles each
    • 100 pmol

CNY 480.00


DDK Rabbit monoclonal antibody, recognizing both N- and C-terminal tags
    • 30 ul

CNY 300.00

Specifications

Product Data
Type Human Tagged ORF Clone
Tag Myc-DDK
Vector pCMV6-Entry
E. coli Selection Kanamycin (25 ug/mL)
Mammalian Cell Selection Neomycin
Sequence Data
>RC227133 representing NM_001145115
Red=Cloning site Blue=ORF Green=Tags(s)

TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC
GCCGCGATCGCC

ATGGAGCCCATAGGGGCGCGGCTAAGTTTGGAGGCGCCGGGACCAGCGCCCTTCCGAGAGGCCCCGCCGG
CCGAGGAGCTGCCCGCCCCGGTGGTCCCCTGTGTGCAGGGTGGCGGCGACGGCGGTGGCGCTTCGGAGAC
CCCGAGTCCTGACGCTCAGCTAGGGGATAGGCCCCTGTCCCCGAAGGAAGAGGCCGCCCCCCAGGAGCAG
GAGGAGCTGCTGGAATGCCGCCGCCGCTGCCGCGCGCGCTCCTTTTCCTTGCCCGCCGACCCCATCTTGC
AGGCGGCCAAGTTCCTGCAGCAGCAGCAGCAACAGGCGGTGGCACTGGGCGGCGAGGGGGCGGAGGACGC
ACAGCTCGGCCCGGGCGGCTGCTGCGCCAAGTGCAAGAAGCGGGTGCAGTTCGCGGACACGCTGGGGCTA
AGCCTGGCCAGCGTGAAGCACTTCAGCGAGGCGGAGGAGCCGCAGGTGCCGCCCGCCGTGCTCTCGCGCC
TGCGAAGCTTCCCTATGCGTGCCGAGGACCTGGAGCAGCTCGGGGGGCTGCTGGCCGCGGCGGCAGTGGC
CGCGCCCCTTTCAGCGCCGCCTTCCCGGCTCCGGCCGCTCTTCCAGCTCCCGGGGCCGAGCGCCGCGGCC
GAGCGTCTGCAGCGGCAGCGCGTGTGCCTGGAGCGCGTGCAGTGCTCGACGGCCTCGGGCGCTGAGGTGA
AGGGCTCCGGCCGGGTGCTCAGCTGCCCTGGGCCCAGGGCCGTGACCGTGCGCTACACCTTTACCGAGTG
GCGCTCCTTCCTGGACGTGCCGGCTGAGCTGCAGCCCGAGCCGCTGGAGCCACAGCAGCCAGAGGCACCG
TCTGGGGCCTCCGAGCCAGGGTCCGGGGATGCCAAGAAAGAGCCAGGCGCCGAGTGCTTCCACTTCTCGC
TGTGCCTGCCCCCGGGCCTGCAGCCTGAGGACGAAGAGGACGCCGACGAGCGCGGCGTCGCGGTCCACTT
CGCTGTCTGCTACCGCTGCGCGCAGGGCGAGTACTGGGACAACAACGCGGGCGCCAACTACACGCTGCGC
TACGCGCGCCCTGCGGACGCGCTC


ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT
ACAAGGATGACGACGATAAG
GTTTAA
>RC227133 representing NM_001145115
Red=Cloning site Green=Tags(s)

MEPIGARLSLEAPGPAPFREAPPAEELPAPVVPCVQGGGDGGGASETPSPDAQLGDRPLSPKEEAAPQEQ
EELLECRRRCRARSFSLPADPILQAAKFLQQQQQQAVALGGEGAEDAQLGPGGCCAKCKKRVQFADTLGL
SLASVKHFSEAEEPQVPPAVLSRLRSFPMRAEDLEQLGGLLAAAAVAAPLSAPPSRLRPLFQLPGPSAAA
ERLQRQRVCLERVQCSTASGAEVKGSGRVLSCPGPRAVTVRYTFTEWRSFLDVPAELQPEPLEPQQPEAP
SGASEPGSGDAKKEPGAECFHFSLCLPPGLQPEDEEDADERGVAVHFAVCYRCAQGEYWDNNAGANYTLR
YARPADAL

TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Chromatograms CHROMATOGRAMS
Sequencher program is needed, download here.
Restriction Sites SgfI-MluI      Cloning Scheme for this gene      Plasmid Map     
ACCN NM_001145115
ORF Size 1074 bp
OTI Disclaimer The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution 1. Centrifuge at 5,000xg for 5min.
2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA.
3. Close the tube and incubate for 10 minutes at room temperature.
4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom.
5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq NM_001145115.1, NP_001138587.1
RefSeq ORF 1077 bp
Locus ID 648791
UniProt ID B7ZBB8
MW 37.8 kDa
Gene Summary Glycogen-targeting subunit for protein phosphatase 1 (PP1). Involved in the regulation of hepatic glycogenesis in a manner coupled to the fasting-feeding cycle and distinct from other glycogen-targeting subunits (By similarity).[UniProtKB/Swiss-Prot Function]
*Delivery time may vary from web posted schedule. Occasional delays may occur due to unforeseen complexities in the preparation of your product. International customers may expect an additional 1-2 weeks in shipping.

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