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SP3 (NM_001172712) Human Tagged ORF Clone
CAT＃: RC230490
- TrueORF®
SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 3
ORF Plasmid: tGFP
"NM_001172712" in other vectors (4)
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CNY 3,990.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | SPR2 |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230490 representing NM_001172712
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACCGCTCCCGAAAAGCCCGTGAAACAAGAGGAAATGGCTGCCTTGGACGTGGATAGCGGCGGCGGCG GTGGCGGCGGCGGCGGCCACGGCGAGTATCTGCAGCAGCAGCAACAGCACGGAAACGGTGCGGTGGCGGC GGCAGCGGCGGCCCAGCCGTCACCGCTCGCTCTGCTGGCCGCTACCTGCAGCAAGATAGGGCCGCCATCG CCGGGCGACGACGAGGAGGAGGCGGCCGCCGCAGCCGGGGCCCCCGCCGCCGCCGGAGCGACAGGTGATT TGGCTTCTGCACAGTTAGGAGGAGCACCAAACCGATGGGAGGTTTTGTCAGCCACACCTACAACTATAAA AGATGAAGCTGGTAATCTAGTCCAGATTCCAAGTGCTGCTACTTCAAGTGGGCAGTATGTTCTTCCCCTT CAGAATTTGCAGAATCAACAAATATTTTCCGTTGCACCAGGATCAGATTCATCAAATGGTACAGTGTCCA GTGTTCAATATCAAGTGATACCACAGATCCAGTCAGCAGATGGTCAGCAGGTTCAAATTGGTTTCACAGG CTCTTCAGATAATGGGGGTATAAATCAAGAAAGCAGTCAAATTCAGATCATTCCTGGCTCTAATCAAACC TTACTTGCCTCTGGAACACCTTCTGCTAACATCCAGAATCTCATACCACAGACTGGTCAAGTCCAGGTTC AGGGAGTTGCAATTGGTGGTTCATCTTTTCCTGGTCAAACCCAAGTAGTTGCTAATGTGCCTCTTGGTCT GCCAGGAAATATTACGTTTGTACCAATCAATAGTGTCGATCTAGATTCTTTGGGACTCTCGGGCAGTTCT CAGACAATGACTGCAGGCATTAATGCCGACGGACATTTGATAAACACAGGACAAGCTATGGATAGTTCAG ACAATTCAGAAAGGACTGGTGAGCGGGTTTCTCCTGATATTAATGAAACTAATACTGATACAGATTTATT TGTGCCAACATCCTCTTCATCACAGTTGCCTGTTACGATAGATAGTACAGGTATATTACAACAAAACACA AATAGCTTGACTACATCTAGTGGGCAGGTTCATTCTTCAGATCTTCAGGGAAATTATATCCAGTCGCCTG TTTCTGAAGAGACACAGGCACAGAATATTCAGGTTTCTACAGCACAGCCTGTTGTACAGCATCTACAACT TCAAGAGTCTCAGCAGCCAACCAGTCAAGCCCAAATTGTGCAAGGTATTACACCACAGACAATCCATGGT GTGCAAGCCAGTGGTCAAAATATATCACAACAGGCTTTGCAAAATCTTCAGTTGCAGCTGAATCCTGGAA CCTTTTTAATTCAGGCACAGACAGTGACCCCTTCTGGACAGGTAACTTGGCAAACGTTTCAAGTACAAGG GGTCCAGAACTTGCAGAATTTGCAAATACAGAATACTGCTGCCCAACAAATAACTTTGACGCCTGTTCAA ACCCTCACACTTGGTCAAGTTGCGGCAGGTGGAGCCTTCACTTCAACTCCAGTTAGTCTAAGCACTGGTC AGTTGCCAAATCTACAAACAGTTACAGTGAACTCTATAGATTCTGCTGGTATACAGCTACATCCAGGAGA GAATGCTGACAGTCCTGCAGATATTAGGATCAAGGAAGAAGAACCTGATCCTGAAGAGTGGCAGCTCAGT GGTGATTCTACCTTGAATACCAATGACCTAACACACTTAAGAGTACAGGTGGTAGATGAAGAAGGGGACC AACAACATCAAGAAGGAAAAAGACTTCGGAGGGTAGCTTGCACCTGTCCCAACTGTAAAGAAGGTGGTGG AAGAGGTACCAATCTTGGGAAAAAGAAGCAACACATTTGTCATATACCAGGATGTGGTAAAGTCTATGGG AAGACCTCACATCTGAGAGCTCATCTGCGTTGGCATTCTGGAGAACGCCCTTTTGTTTGTAACTGGATGT ACTGTGGTAAAAGATTTACTCGAAGTGATGAATTACAGAGGCACAGAAGAACACATACAGGTGAGAAGAA ATTTGTTTGTCCAGAATGTTCAAAACGCTTTATGAGAAGTGACCACCTTGCCAAACATATTAAAACACAC CAGAATAAAAAAGGTATTCACTCTAGCAGTACAGTGCTGGCATCTGTGGAAGCTGCGCGAGATGATACTT TGATTACTGCAGGAGGAACAACGCTTATCCTTGCAAATATTCAACAAGGTTCTGTTTCAGGGATAGGAAC TGTTAATACTTCCGCCACCAGCAATCAAGATATCCTTACCAACACTGAAATACCTTTACAGCTTGTCACA GTTTCTGGAAATGAGACAATGGAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230490 representing NM_001172712
Red=Cloning site Green=Tags(s) MTAPEKPVKQEEMAALDVDSGGGGGGGGGHGEYLQQQQQHGNGAVAAAAAAQPSPLALLAATCSKIGPPS PGDDEEEAAAAAGAPAAAGATGDLASAQLGGAPNRWEVLSATPTTIKDEAGNLVQIPSAATSSGQYVLPL QNLQNQQIFSVAPGSDSSNGTVSSVQYQVIPQIQSADGQQVQIGFTGSSDNGGINQESSQIQIIPGSNQT LLASGTPSANIQNLIPQTGQVQVQGVAIGGSSFPGQTQVVANVPLGLPGNITFVPINSVDLDSLGLSGSS QTMTAGINADGHLINTGQAMDSSDNSERTGERVSPDINETNTDTDLFVPTSSSSQLPVTIDSTGILQQNT NSLTTSSGQVHSSDLQGNYIQSPVSEETQAQNIQVSTAQPVVQHLQLQESQQPTSQAQIVQGITPQTIHG VQASGQNISQQALQNLQLQLNPGTFLIQAQTVTPSGQVTWQTFQVQGVQNLQNLQIQNTAAQQITLTPVQ TLTLGQVAAGGAFTSTPVSLSTGQLPNLQTVTVNSIDSAGIQLHPGENADSPADIRIKEEEPDPEEWQLS GDSTLNTNDLTHLRVQVVDEEGDQQHQEGKRLRRVACTCPNCKEGGGRGTNLGKKKQHICHIPGCGKVYG KTSHLRAHLRWHSGERPFVCNWMYCGKRFTRSDELQRHRRTHTGEKKFVCPECSKRFMRSDHLAKHIKTH QNKKGIHSSSTVLASVEAARDDTLITAGGTTLILANIQQGSVSGIGTVNTSATSNQDILTNTEIPLQLVT VSGNETME TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001172712 |
| ORF Size | 2334 bp |
| OTI Disclaimer | The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001172712.1, NP_001166183.1 |
| RefSeq ORF | 2337 bp |
| Locus ID | 6670 |
| UniProt ID | Q02447 |
| Protein Families | Druggable Genome, Transcription Factors |
| MW | 82 kDa |
| Gene Summary | This gene belongs to a family of Sp1 related genes that encode transcription factors that regulate transcription by binding to consensus GC- and GT-box regulatory elements in target genes. This protein contains a zinc finger DNA-binding domain and several transactivation domains, and has been reported to function as a bifunctional transcription factor that either stimulates or represses the transcription of numerous genes. Transcript variants encoding different isoforms have been described for this gene, and one has been reported to initiate translation from a non-AUG (AUA) start codon. Additional isoforms, resulting from the use of alternate downstream translation initiation sites, have also been noted. A related pseudogene has been identified on chromosome 13. [provided by RefSeq, Feb 2010] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230490L3 | Lenti-ORF clone of SP3 (Myc-DDK-tagged)-Human Sp3 transcription factor (SP3), transcript variant 3 |
CNY 5,890.00 |
|
| RC230490L4 | Lenti-ORF clone of SP3 (mGFP-tagged)-Human Sp3 transcription factor (SP3), transcript variant 3 |
CNY 5,890.00 |
|
| RG230490 | SP3 (tGFP-tagged) - Human Sp3 transcription factor (SP3), transcript variant 3 |
CNY 4,370.00 |
|
| SC329128 | SP3 (untagged)-Human Sp3 transcription factor (SP3) transcript variant 3 |
CNY 7,220.00 |
