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eIF4GII (EIF4G3) (NM_001198802) Human Tagged ORF Clone
CAT＃: RC230898
- TrueORF®
EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2
ORF Plasmid: tGFP
"NM_001198802" in other vectors (5)
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CNY 15,870.00
Product images
CNY 300.00
Specifications
| Product Data | |
| Type | Human Tagged ORF Clone |
| Tag | Myc-DDK |
| Synonyms | eIF-4G 3; eIF4G 3; eIF4GII |
| Vector | pCMV6-Entry |
| E. coli Selection | Kanamycin (25 ug/mL) |
| Mammalian Cell Selection | Neomycin |
| Sequence Data |
>RC230898 representing NM_001198802
Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGAATTCACAACCTCAAACCCGTTCTCCGGGAGGATTCAGACCTATCCAGTTTTTCCAGAGGCCTCAAA TACAGCCTCCTAGAGCTACCATCCCGAACAGCAGTCCTTCCATTCGTCCTGGTGCACAGACACCCACTGC AGTGTACCAGGCTAATCAGCACATCATGATGGTTAACCATCTGCCCATGCCGTACCCAGTGCCCCAGGGG CCTCAGTACTGTATACCACAGTACCGTCATAGTGGCCCTCCTTATGTTGGGCCCCCCCAACAATATCCAG TTCAACCACCGGGGCCAGGTCCTTTTTATCCTGGACCAGGACCTGGGGACTTCCCCAATGCTTATGGAAC GCCTTTTTACCCAAGTCAGCCGGTGTATCAGTCAGCACCTATCATAGTGCCTACGCAGCAACAGCCGCCT CCAGCCAAGAGAGAGAAAAAAACTATAAGAATTCGGGATCCAAACCAGGGAGGTAAAGACATAACAGAGG AGATTATGTCTGGAGGTGGCAGCAGAAATCCTACTCCACCCATAGGAAGACCCACGTCCACACCTACTCC TCCTCAGCTGCCCAGCCAGGTCCCCGAGCACAGCCCTGTGGTTTATGGGACTGTGGAGAGCGCTCATCTT GCTGCCAGCACCCCTGTCACTGCAGCTAGCGACCAGAAGCAAGAGGAGAAGCCAAAACCAGATCCAGTGT TAAAGTCTCCTTCCCCAGTCCTTAGGCTAGTCCTCAGTGGAGAGAAGAAAGAACAAGAAGGCCAGACATC TGAAACTACTGCAATAGTATCCATAGCAGAGCTTCCTCTGCCTCCATCACCTACCACTGTTTCTTCTGTT GCTCGAAGTACAATTGCAGCCCCCACCTCTTCTGCTCTTAGTAGCCAACCAATATTCACCACTGCTATAG ATGACAGATGTGAACTCTCATCCCCAAGAGAAGACACAATTCCTATACCCAGCCTCACATCTTGCACAGA AACATCAGACCCTTTACCAACAAATGAAAATGATGATGATATATGCAAGAAACCCTGTAGTGTAGCACCT AATGATATTCCACTGGTTTCTAGTACTAACCTAATTAATGAAATAAATGGAGTTAGCGAAAAATTATCAG CCACGGAGAGCATTGTGGAAATAGTAAAACAGGAAGTATTGCCATTGACTCTTGAATTGGAGATTCTCGA AAATCCCCCAGAAGAAATGAAACTGGAGTGTATCCCAGCTCCCATCACCCCTTCCACAGTTCCTTCCTTT CCTCCAACTCCTCCAACTCCTCCAGCTTCTCCTCCTCACACTCCAGTCATTGTTCCTGCTGCTGCCACTA CTGTTAGTTCTCCGAGTGCTGCCATCACAGTCCAGAGAGTCCTAGAGGAGGACGAGAGCATAAGAACTTG CCTTAGTGAAGATGCAAAAGAGATTCAGAACAAAATAGAGGTAGAAGCAGATGGGCAAACAGAAGAGATT TTGGATTCTCAAAACTTAAATTCAAGAAGGAGCCCTGTCCCAGCTCAAATAGCTATAACTGTACCAAAGA CATGGAAGAAACCAAAAGATCGGACCCGAACCACTGAAGAGATGTTAGAGGCAGAATTGGAGCTTAAAGC TGAAGAGGAGCTTTCCATTGACAAAGTACTTGAATCTGAACAAGATAAAATGAGCCAGGGGTTTCATCCT GAAAGAGACCCCTCTGACCTAAAAAAAGTGAAAGCTGTGGAAGAAAATGGAGAAGAAGCTGAGCCAGTAC GTAATGGTGCTGAGAGTGTTTCTGAGGGTGAAGGAATAGATGCTAATTCAGGCTCCACAGATAGTTCTGG TGATGGGGTTACATTTCCATTTAAACCAGAATCCTGGAAGCCTACTGATACTGAAGGTAAGAAGCAGTAT GACAGGGAGTTTCTGCTGGACTTCCAGTTCATGCCTGCCTGTATACAAAAACCAGAGGGCCTGCCTCCTA TCAGTGATGTGGTTCTTGACAAGATCAACCAACCCAAATTGCCAATGCGAACTCTGGATCCTCGAATTTT GCCTCGAGGACCAGACTTTACACCAGCCTTTGCTGATTTTGGAAGGCAGACACCTGGTGGAAGAGGCGTA CCTTTGTTGAATGTTGGGTCACGAAGATCTCAACCTGGCCAAAGAAGAGAACCCAGAAAGATCATCACAG TTTCTGTAAAAGAAGATGTACACCTGAAAAAGGCAGAAAATGCCTGGAAGCCAAGCCAAAAACGAGACAG CCAAGCCGATGATCCCGAAAACATTAAAACCCAGGAGCTTTTTAGAAAAGTTCGAAGTATCTTAAATAAA TTGACACCACAGATGTTCAATCAACTGATGAAGCAAGTGTCAGGACTTACTGTTGACACAGAGGAGCGGC TGAAAGGAGTTATTGACCTGGTCTTTGAGAAGGCTATTGATGAACCCAGTTTCTCTGTGGCTTACGCAAA CATGTGTCGATGTCTAGTAACGCTGAAAGTACCCATGGCAGACAAGCCTGGTAACACAGTGAATTTCCGG AAGCTGCTACTGAACCGTTGCCAGAAGGAGTTTGAAAAAGATAAAGCAGATGATGATGTCTTTGAGAAGA AGCAGAAAGAACTTGAGGCTGCCAGTGCTCCAGAGGAGAGGACAAGGCTTCATGATGAACTGGAAGAAGC CAAGGACAAAGCCCGGCGGAGATCCATTGGCAACATCAAGTTTATTGGAGAACTCTTTAAACTCAAAATG CTGACTGAAGCCATCATGCATGACTGTGTGGTGAAGCTGCTAAAGAACCATGATGAAGAATCCCTGGAGT GCCTGTGTCGCCTGCTCACCACCATTGGCAAAGACTTGGACTTTGAAAAAGCAAAGCCACGTATGGACCA GTACTTTAATCAGATGGAGAAAATTGTGAAAGAAAGAAAAACCTCATCTAGGATTCGGTTCATGCTTCAA GATGTTATAGACCTAAGGCTGTGCAATTGGGTATCTCGAAGAGCAGATCAAGGGCCTAAAACTATCGAAC AGATTCACAAAGAGGCTAAAATAGAAGAACAAGAAGAGCAAAGGAAGGTCCAGCAACTCATGACCAAAGA GAAGAGAAGACCAGGTGTCCAGAGAGTGGACGAAGGTGGGTGGAACACTGTACAAGGGGCCAAGAACAGT CGGGTACTGGACCCCTCAAAATTCCTAAAAATCACTAAGCCTACAATTGATGAAAAAATTCAGCTGGTAC CTAAAGCACAGCTAGGCAGCTGGGGAAAAGGCAGCAGTGGTGGAGCAAAGGCAAGTGAGACTGATGCCTT ACGGTCAAGTGCTTCCAGTTTAAACAGATTCTCTGCCCTGCAACCTCCAGCACCCTCAGGGTCCACGCCA TCCACGCCTGTAGAGTTTGATTCCCGAAGGACCTTAACTAGTCGTGGAAGTATGGGCAGGGAGAAGAATG ACAAGCCCCTTCCATCTGCAACAGCTCGGCCAAATACTTTCATGAGGGGTGGCAGCAGTAAAGACCTGCT AGACAATCAGTCTCAAGAAGAGCAGCGGAGAGAGATGCTGGAGACCGTGAAGCAGCTCACAGGAGGTGTG GATGTGGAGAGGAACAGCACTGAGGCTGAGCGAAATAAAACAAGGGAGTCAGCAAAACCAGAAATTTCAG CAATGTCAGCTCATGACAAGGCTGCATTATCAGAAGAGGAACTGGAGAGGAAGTCGAAATCTATCATTGA TGAATTTCTACACATTAATGATTTTAAGGAAGCCATGCAGTGTGTGGAAGAGCTGAATGCCCAGGGCCTA CTACATGTTTTTGTGAGAGTGGGAGTGGAGTCCACCCTGGAAAGGAGCCAGATCACCAGGGATCACATGG GCCAACTACTCTATCAGCTGGTACAGTCAGAAAAACTCAGCAAACAGGACTTTTTCAAAGGTTTTTCAGA AACTTTGGAATTGGCAGATGACATGGCCATTGATATTCCCCATATTTGGTTGTACCTTGCTGAACTGGTG ACCCCCATGTTAAAAGAAGGTGGAATCTCCATGAGAGAACTTACCATAGAATTTAGCAAACCTTTACTTC CTGTTGGAAGAGCTGGGGTCTTGCTATCTGAAATATTGCACCTACTATGCAAACAAATGAGCCATAAGAA AGTGGGAGCCTTATGGAGGGAGGCTGACCTCAGCTGGAAGGACTTTTTACCAGAAGGAGAAGATGTACAT AATTTTCTTTTGGAGCAGAAGTTGGACTTCATAGAGTCTGACAGTCCCTGTTCCTCTGAAGCACTTTCAA AGAAAGAACTGTCTGCCGAAGAGCTGTATAAGCGACTCGAGAAACTCATTATTGAGGACAAAGCGAATGA TGAACAGATCTTTGACTGGGTAGAGGCTAATCTAGACGAAATCCAGATGAGTTCACCTACATTCCTTAGA GCTTTAATGACTGCTGTTTGTAAAGCAGCTATTATAGCCGACTCTTCTACCTTCAGAGTGGACACTGCTG TTATCAAGCAGAGAGTGCCGATCTTACTCAAGTACCTAGACTCAGATACAGAGAAGGAACTGCAAGCACT TTATGCACTACAAGCATCGATAGTAAAACTTGATCAACCTGCCAATTTGCTGCGGATGTTTTTTGATTGT CTATATGACGAGGAGGTGATCTCCGAGGATGCCTTCTACAAATGGGAGAGCAGCAAGGACCCTGCAGAGC AGAATGGGAAGGGCGTGGCTCTGAAATCTGTCACGGCATTCTTCACGTGGCTGCGGGAAGCAGAAGAGGA GTCTGAGGATAAC ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA >RC230898 representing NM_001198802
Red=Cloning site Green=Tags(s) MNSQPQTRSPGGFRPIQFFQRPQIQPPRATIPNSSPSIRPGAQTPTAVYQANQHIMMVNHLPMPYPVPQG PQYCIPQYRHSGPPYVGPPQQYPVQPPGPGPFYPGPGPGDFPNAYGTPFYPSQPVYQSAPIIVPTQQQPP PAKREKKTIRIRDPNQGGKDITEEIMSGGGSRNPTPPIGRPTSTPTPPQLPSQVPEHSPVVYGTVESAHL AASTPVTAASDQKQEEKPKPDPVLKSPSPVLRLVLSGEKKEQEGQTSETTAIVSIAELPLPPSPTTVSSV ARSTIAAPTSSALSSQPIFTTAIDDRCELSSPREDTIPIPSLTSCTETSDPLPTNENDDDICKKPCSVAP NDIPLVSSTNLINEINGVSEKLSATESIVEIVKQEVLPLTLELEILENPPEEMKLECIPAPITPSTVPSF PPTPPTPPASPPHTPVIVPAAATTVSSPSAAITVQRVLEEDESIRTCLSEDAKEIQNKIEVEADGQTEEI LDSQNLNSRRSPVPAQIAITVPKTWKKPKDRTRTTEEMLEAELELKAEEELSIDKVLESEQDKMSQGFHP ERDPSDLKKVKAVEENGEEAEPVRNGAESVSEGEGIDANSGSTDSSGDGVTFPFKPESWKPTDTEGKKQY DREFLLDFQFMPACIQKPEGLPPISDVVLDKINQPKLPMRTLDPRILPRGPDFTPAFADFGRQTPGGRGV PLLNVGSRRSQPGQRREPRKIITVSVKEDVHLKKAENAWKPSQKRDSQADDPENIKTQELFRKVRSILNK LTPQMFNQLMKQVSGLTVDTEERLKGVIDLVFEKAIDEPSFSVAYANMCRCLVTLKVPMADKPGNTVNFR KLLLNRCQKEFEKDKADDDVFEKKQKELEAASAPEERTRLHDELEEAKDKARRRSIGNIKFIGELFKLKM LTEAIMHDCVVKLLKNHDEESLECLCRLLTTIGKDLDFEKAKPRMDQYFNQMEKIVKERKTSSRIRFMLQ DVIDLRLCNWVSRRADQGPKTIEQIHKEAKIEEQEEQRKVQQLMTKEKRRPGVQRVDEGGWNTVQGAKNS RVLDPSKFLKITKPTIDEKIQLVPKAQLGSWGKGSSGGAKASETDALRSSASSLNRFSALQPPAPSGSTP STPVEFDSRRTLTSRGSMGREKNDKPLPSATARPNTFMRGGSSKDLLDNQSQEEQRREMLETVKQLTGGV DVERNSTEAERNKTRESAKPEISAMSAHDKAALSEEELERKSKSIIDEFLHINDFKEAMQCVEELNAQGL LHVFVRVGVESTLERSQITRDHMGQLLYQLVQSEKLSKQDFFKGFSETLELADDMAIDIPHIWLYLAELV TPMLKEGGISMRELTIEFSKPLLPVGRAGVLLSEILHLLCKQMSHKKVGALWREADLSWKDFLPEGEDVH NFLLEQKLDFIESDSPCSSEALSKKELSAEELYKRLEKLIIEDKANDEQIFDWVEANLDEIQMSSPTFLR ALMTAVCKAAIIADSSTFRVDTAVIKQRVPILLKYLDSDTEKELQALYALQASIVKLDQPANLLRMFFDC LYDEEVISEDAFYKWESSKDPAEQNGKGVALKSVTAFFTWLREAEEESEDN TRTRPLEQKLISEEDLAANDILDYKDDDDKV |
| Restriction Sites |
SgfI-MluI
Cloning Scheme for this gene
Plasmid Map
|
| ACCN | NM_001198802 |
| ORF Size | 4773 bp |
| OTI Disclaimer | Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info |
| OTI Annotation | This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene. |
| Product Components | The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water). |
| Reconstitution | 1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C. |
| Reference Data | |
| RefSeq | NM_001198802.1, NP_001185731.1 |
| RefSeq ORF | 4776 bp |
| Locus ID | 8672 |
| UniProt ID | O43432 |
| Protein Families | Transcription Factors |
| Protein Pathways | Viral myocarditis |
| MW | 177.7 kDa |
| Gene Summary | The protein encoded by this gene is thought to be part of the eIF4F protein complex, which is involved in mRNA cap recognition and transport of mRNAs to the ribosome. Interestingly, a microRNA (miR-520c-3p) has been found that negatively regulates synthesis of the encoded protein, and this leads to a global decrease in protein translation and cell proliferation. Therefore, this protein is a key component of the anti-tumor activity of miR-520c-3p. [provided by RefSeq, May 2016] |
Documents
| Product Manuals |
| FAQs |
| SDS |
Resources
Other Versions
| SKU | Description | Size | Price |
|---|---|---|---|
| RC230898L1 | Lenti-ORF clone of EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2 |
CNY 17,770.00 |
|
| RC230898L2 | Lenti-ORF clone of EIF4G3 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2 |
CNY 17,770.00 |
|
| RC230898L3 | Lenti-ORF clone of EIF4G3 (Myc-DDK-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2 |
CNY 17,770.00 |
|
| RC230898L4 | Lenti-ORF clone of EIF4G3 (mGFP-tagged)-Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2 |
CNY 17,770.00 |
|
| RG230898 | EIF4G3 (tGFP-tagged) - Human eukaryotic translation initiation factor 4 gamma, 3 (EIF4G3), transcript variant 2 |
CNY 17,480.00 |
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