B7 H6 (NCR3LG1) (NM_001202439) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	B7-H6; B7H6; DKFZp686O24166
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC232774 representing NM_001202439 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGACGTGGAGGGCTGCCGCCTCCACGTGCGCGGCGCTCCTGATTCTGCTGTGGGCGCTGACGACCGAAG GTGATCTGAAAGTAGAGATGATGGCAGGGGGGACTCAGATCACACCCCTGAATGACAATGTCACCATATT CTGCAATATCTTTTATTCCCAACCCCTCAACATCACGTCTATGGGTATCACCTGGTTTTGGAAGAGTCTG ACGTTTGACAAAGAAGTCAAAGTCTTTGAATTTTTTGGAGATCACCAAGAGGCATTCCGACCTGGAGCCA TTGTGTCTCCATGGAGGCTGAAGAGTGGGGACGCCTCACTGCGGCTGCCTGGAATCCAGCTGGAGGAAGC AGGAGAGTACCGATGTGAGGTGGTGGTCACCCCTCTGAAGGCACAGGGAACAGTCCAGCTTGAAGTTGTG GCTTCCCCAGCCAGCAGATTGTTGCTGGATCAAGTGGGCATGAAAGAGAATGAAGACAAATATATGTGTG AGTCAAGTGGGTTCTACCCAGAGGCTATTAATATAACATGGGAGAAGCAGACCCAGAAGTTTCCCCATCC CATAGAGATTTCTGAGGATGTCATCACTGGTCCCACCATCAAGAATATGGATGGCACATTTAATGTCACT AGCTGCTTGAAGCTGAACTCCTCTCAGGAAGACCCTGGGACTGTCTACCAGTGTGTGGTACGGCATGCGT CCTTGCATACCCCCTTGAGGAGCAACTTTACCCTGACTGCTGCTCGGCACAGTCTTTCTGAAACTGAGAA GACAGATAATTTTTCCATTCATTGGTGGCCTATTTCATTCATTGGTGTTGGACTGGTTTTATTAATTGTT TTGATTCCTTGGAAAAAGATATGTAACAAATCATCTTCAGCCTATACTCCTCTCAAGTGCATTCTGAAAC ACTGGAACTCCTTTGACACTCAGACTCTGAAGAAAGAGCACCTCATATTCTTTTGCACTCGGGCATGGCC GTCTTACCAGCTGCAGGATGGGGAGGCTTGGCCTCCTGAGGGAAGTGTTAATATTAATACTATTCAACAA CTAGATGTTTTCTGCAGACAGGAGGGCAAATGGTCCGAGGTTCCTTATGTGCAAGCCTTCTTTGCCTTGC GAGACAACCCAGATCTTTGTCAGTGTTGTAGAATTGACCCTGCTCTCCTAACAGTTACATCAGGCAAGTC CATAGATGATAATTCCACAAAGTCTGAGAAACAAACCCCTAGGGAACACTCGGATGCAGTTCCGGATGCC CCAATCCTTCCTGTCTCCCCTATCTGGGAACCTCCTCCAGCCACAACATCAACAACTCCAGTTCTATCCT CCCAACCCCCAACTTTACTGTTACCCCTACAG ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC232774 representing NM_001202439 Red=Cloning site Green=Tags(s) MTWRAAASTCAALLILLWALTTEGDLKVEMMAGGTQITPLNDNVTIFCNIFYSQPLNITSMGITWFWKSL TFDKEVKVFEFFGDHQEAFRPGAIVSPWRLKSGDASLRLPGIQLEEAGEYRCEVVVTPLKAQGTVQLEVV ASPASRLLLDQVGMKENEDKYMCESSGFYPEAINITWEKQTQKFPHPIEISEDVITGPTIKNMDGTFNVT SCLKLNSSQEDPGTVYQCVVRHASLHTPLRSNFTLTAARHSLSETEKTDNFSIHWWPISFIGVGLVLLIV LIPWKKICNKSSSAYTPLKCILKHWNSFDTQTLKKEHLIFFCTRAWPSYQLQDGEAWPPEGSVNINTIQQ LDVFCRQEGKWSEVPYVQAFFALRDNPDLCQCCRIDPALLTVTSGKSIDDNSTKSEKQTPREHSDAVPDA PILPVSPIWEPPPATTSTTPVLSSQPPTLLLPLQ TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001202439
ORF Size	1362 bp
OTI Disclaimer	Due to the inherent nature of this plasmid, standard methods to replicate additional amounts of DNA in E. coli are highly likely to result in mutations and/or rearrangements. Therefore, OriGene does not guarantee the capability to replicate this plasmid DNA. Additional amounts of DNA can be purchased from OriGene with batch-specific, full-sequence verification at a reduced cost. Please contact our customer care team at custsupport@origene.com or by calling 301.340.3188 option 3 for pricing and delivery. The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001202439.3
RefSeq Size	6382 bp
RefSeq ORF	1365 bp
Locus ID	374383
UniProt ID	Q68D85
Protein Families	Transmembrane
MW	51.3 kDa
Gene Summary	B7H6 belongs to the B7 family (see MIM 605402) and is selectively expressed on tumor cells. Interaction of B7H6 with NKp30 (NCR3; MIM 611550) results in natural killer (NK) cell activation and cytotoxicity (Brandt et al., 2009 [PubMed 19528259]).[supplied by OMIM, Jan 2011]

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