TAF1C (NM_001243158) Human Tagged ORF Clone

Specifications

Product Data
Type	Human Tagged ORF Clone
Tag	Myc-DDK
Synonyms	MGC:39976; SL1; TAFI95; TAFI110
Vector	pCMV6-Entry
E. coli Selection	Kanamycin (25 ug/mL)
Mammalian Cell Selection	Neomycin
Sequence Data	ORF Nucleotide Sequence (showhide) >RC234377 representing NM_001243158 Red=Cloning site Blue=ORF Green=Tags(s) TTTTGTAATACGACTCACTATAGGGCGGCCGGGAATTCGTCGACTGGATCCGGTACCGAGGAGATCTGCC GCCGCGATCGCC ATGCAGGTGGAGAAAGGGGCCACGGGGATCAGCCTCAGCCCTCACCTGCCCGGGGAGCTGGCCATCTGCA GCCGCTCGGGAGCCGTCTGCCTGTGGAGCCCTGAGGATGGGCTGCGGCAAATCTACAGGGACCCTGAGAC CCTCGTGTTCCGGGACTCCTCTTCGTGGCGTTGGGCAGACTTCACTGCGCACCCTCGGGTGCTGACCGTG GGTGACCGCACCGGAGTGAAGATGCTGGACACTCAGGGCCCGCCGGGCTGTGGTCTGTTGCTTTTTCGTT TGGGGGCAGAGGCTTCGTGCCAGAAAGGGGAACGTGTCCTGCTTACCCAGTACCTGGGGCACTCCAGCCC CAAATGCCTCCCCCCTACTCTTCATCTCGTCTGTACCCAGTTCTCTCTCTACCTAGTGGACGAGCGCCTT CCCCTGGTGCCGATGCTGAAGTGGAACCATGGCCTCCCCTCCCCGCTCCTGCTGGCCCGACTGCTGCCTC CGCCCCGGCCCAGCTGCGTGCAGCCCCTGCTCCTCGGAGGCCAGGGTGGGCAGCTGCAGCTGCTGCACCT GGCAGGAGAAGGGGCGTCGGTGCCCCGCCTGGCAGGCCCCCCCCAGTCTCTTCCTTCCAGGATCGACTCC CTCCCTGCATTTCCTCTGCTGGAGCCTAAGATCCAGTGGCGGCTGCAGGAGCGCCTGAAAGCACCGACCA TAGGTCTGGCTGCCGTCGTCCCGCCCTTGCCCTCAGCGCCCACACCAGGCCTGGTGCTCTTCCAGCTCTC GGCGGCGGGAGATGTCTTCTACCAGCAGCTCCGCCCCCAGGTGGACTCCAGCCTCCGCAGAGATGCTGGG CCTCCTGGCGACACCCAACCTGACTGCCATGCCCCCACAGCTTCCTGGACCTCCCAGGACACTGCCGGCT GCAGCCAGTGGCTGAAGGCCCTGCTAAAAGTGCCCCTGGCTCCTCCTGTGTGGACAGCACCCACCTTCAC CCACCGCCAGATGCTGGGCAGCACAGAGCTGCGGAGGGAGGAAGAGGAAGGGCAGCGGCTGGGTGTGCTC CGCAAGGCCATGGCCCGAGGGCAGCTCCTGCTGCAGAGAGACCTGGGCTCCCTCCCTGCGGCAGAGCCAC CCCCTGCACCCGAGTCAGGCCTAGAGGACAAGCTCAGTGAGCGCCTGGGGGAAGCCTGGGCAGGCCGAGG GGCTGCCTGGTGGGAGAGGCAGCAGGGCAGGACCTCGGAGCCCGGGAGACAGACCAGGCGGCCCAAGCGC CGGACCCAGCTGTCCAGCAGCTTTTCGCTCAGTGGCCATGTGGATCCCTCAGAGGACACCAGCTCCCCTC ATAGCCCTGAGTGGCCACCTGCTGATGCTCTGCCCCTGCCCCCCACGACCCCGCCCTCCCAGGAGTTGAC TCCGGATGCATGCGCCCAGGGCGTCCCATCAGAGCAGCGGCAGATGCTCCGTGACTACATGGCCAAGCTA CCACCCCAGAGGGACACCCCAGGCTGTGCCACCACACCTCCCCACTCCCAGGCCTCCAGCGTCCGGGCCA CTCGCTCCCAGCAGCACACACCCGTCCTCTCTAGCTCTCAGCCCCTCCGGAAGAAGCCTCGAATGGGCTT C ACGCGTACGCGGCCGCTCGAGCAGAAACTCATCTCAGAAGAGGATCTGGCAGCAAATGATATCCTGGATT ACAAGGATGACGACGATAAGGTTTAA Protein Sequence (showhide) >RC234377 representing NM_001243158 Red=Cloning site Green=Tags(s) MQVEKGATGISLSPHLPGELAICSRSGAVCLWSPEDGLRQIYRDPETLVFRDSSSWRWADFTAHPRVLTV GDRTGVKMLDTQGPPGCGLLLFRLGAEASCQKGERVLLTQYLGHSSPKCLPPTLHLVCTQFSLYLVDERL PLVPMLKWNHGLPSPLLLARLLPPPRPSCVQPLLLGGQGGQLQLLHLAGEGASVPRLAGPPQSLPSRIDS LPAFPLLEPKIQWRLQERLKAPTIGLAAVVPPLPSAPTPGLVLFQLSAAGDVFYQQLRPQVDSSLRRDAG PPGDTQPDCHAPTASWTSQDTAGCSQWLKALLKVPLAPPVWTAPTFTHRQMLGSTELRREEEEGQRLGVL RKAMARGQLLLQRDLGSLPAAEPPPAPESGLEDKLSERLGEAWAGRGAAWWERQQGRTSEPGRQTRRPKR RTQLSSSFSLSGHVDPSEDTSSPHSPEWPPADALPLPPTTPPSQELTPDACAQGVPSEQRQMLRDYMAKL PPQRDTPGCATTPPHSQASSVRATRSQQHTPVLSSSQPLRKKPRMGF TRTRPLEQKLISEEDLAANDILDYKDDDDKV
Restriction Sites	SgfI-MluI      Cloning Scheme for this gene      Plasmid Map
ACCN	NM_001243158
ORF Size	1611 bp
OTI Disclaimer	The molecular sequence of this clone aligns with the gene accession number as a point of reference only. However, individual transcript sequences of the same gene can differ through naturally occurring variations (e.g. polymorphisms), each with its own valid existence. This clone is substantially in agreement with the reference, but a complete review of all prevailing variants is recommended prior to use. More info
OTI Annotation	This clone was engineered to express the complete ORF with an expression tag. Expression varies depending on the nature of the gene.
Product Components	The ORF clone is ion-exchange column purified and shipped in a 2D barcoded Matrix tube containing 10ug of transfection-ready, dried plasmid DNA (reconstitute with 100 ul of water).
Reconstitution	1. Centrifuge at 5,000xg for 5min. 2. Carefully open the tube and add 100ul of sterile water to dissolve the DNA. 3. Close the tube and incubate for 10 minutes at room temperature. 4. Briefly vortex the tube and then do a quick spin (less than 5000xg) to concentrate the liquid at the bottom. 5. Store the suspended plasmid at -20°C. The DNA is stable for at least one year from date of shipping when stored at -20°C.
Reference Data
RefSeq	NM_001243158.2
RefSeq Size	3379 bp
RefSeq ORF	1614 bp
Locus ID	9013
UniProt ID	Q15572
Protein Families	Transcription Factors
MW	58.9 kDa
Gene Summary	Initiation of transcription by RNA polymerase I requires the formation of a complex composed of the TATA-binding protein (TBP) and three TBP-associated factors (TAFs) specific for RNA polymerase I. This complex, known as SL1, binds to the core promoter of ribosomal RNA genes to position the polymerase properly and acts as a channel for regulatory signals. This gene encodes the largest SL1-specific TAF. Multiple alternatively spliced transcript variants encoding different isoforms have been identified. [provided by RefSeq, Jul 2011]

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